東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Polymer-DNA hybrids: Molecular recog...

Davis, Julianne Montfort Gibbs.

FindBook

Google Book

Amazon

博客來

Polymer-DNA hybrids: Molecular recognition properties and applications in DNA detection.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Polymer-DNA hybrids: Molecular recognition properties and applications in DNA detection./
作者:	Davis, Julianne Montfort Gibbs.
面頁冊數:	239 p.
附註:	Adviser: SonBinh T. Nguyen.
Contained By:	Dissertation Abstracts International67-03B.
標題:	Biophysics, General. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3212832
ISBN:	9780542622038

Polymer-DNA hybrids: Molecular recognition properties and applications in DNA detection.
Davis, Julianne Montfort Gibbs.

Polymer-DNA hybrids: Molecular recognition properties and applications in DNA detection. - 239 p.

Adviser: SonBinh T. Nguyen.

Thesis (Ph.D.)--Northwestern University, 2006.

Ring-opening metathesis polymerization was used to make nearly monodisperse, comb, block copolymer-DNA hybrids with DNA strands and redox-active ferrocenyl groups as the side-chains. By varying the ratio of two ferrocenyl blocks in the polymer, triblock copolymer DNA hybrids were formed that had distinctive, electrochemical signals, allowing them to be used as highly selective probes in DNA detection assays. The selectivity stemmed from the very sharp dissociation, or melting, transitions observed for aggregates formed from complementary polymer-DNA hybrids. Structure-variation studies of the homopolymer-DNA hybrids revealed that neither changing the polymer length, nor increasing the spacer sequence connecting the polymer backbone to the hybridizing sequence, had any affect on the melting behavior. A comparison of the hybridization behavior of our polymer-DNA system with that of organic, small molecule-DNA hybrids (containing 1--4 DNA strands depending on the structure) suggested that both the presence of multiple DNA strands on the polymer-DNA hybrids as well as their geometrical arrangement were important parameters that strongly influence melting behavior.

ISBN: 9780542622038Subjects--Topical Terms:

1019105
Biophysics, General.

Polymer-DNA hybrids: Molecular recognition properties and applications in DNA detection.
LDR:03298nam 2200313 a 45 001 967955
005 20110915
008 110915s2006 eng d
020 $a 9780542622038
035 $a (UMI)AAI3212832
035 $a AAI3212832
040 $a UMI $c UMI
100 1 $a Davis, Julianne Montfort Gibbs. $3 1291824
245 1 0 $a Polymer-DNA hybrids: Molecular recognition properties and applications in DNA detection.
300 $a 239 p.
500 $a Adviser: SonBinh T. Nguyen.
500 $a Source: Dissertation Abstracts International, Volume: 67-03, Section: B, page: 1465.
502 $a Thesis (Ph.D.)--Northwestern University, 2006.
520 $a Ring-opening metathesis polymerization was used to make nearly monodisperse, comb, block copolymer-DNA hybrids with DNA strands and redox-active ferrocenyl groups as the side-chains. By varying the ratio of two ferrocenyl blocks in the polymer, triblock copolymer DNA hybrids were formed that had distinctive, electrochemical signals, allowing them to be used as highly selective probes in DNA detection assays. The selectivity stemmed from the very sharp dissociation, or melting, transitions observed for aggregates formed from complementary polymer-DNA hybrids. Structure-variation studies of the homopolymer-DNA hybrids revealed that neither changing the polymer length, nor increasing the spacer sequence connecting the polymer backbone to the hybridizing sequence, had any affect on the melting behavior. A comparison of the hybridization behavior of our polymer-DNA system with that of organic, small molecule-DNA hybrids (containing 1--4 DNA strands depending on the structure) suggested that both the presence of multiple DNA strands on the polymer-DNA hybrids as well as their geometrical arrangement were important parameters that strongly influence melting behavior.
520 $a When polymer-DNA hybrids were hybridized with complementary DNA-modified gold nanoparticles in a three-strand experiment, the melting behavior of the mixed hybrid system was similar to the behavior of the polymer-DNA and DNA-modified gold nanoparticle homoaggregates, with an intermediate melting temperature and sharpness. When a peptide nucleic acid (PNA) strand was substituted for the linker strand, both the PNA-linked polymer-DNA and GNP-DNA homoaggregates displayed sharp melting transitions and unusual salt dependence.
520 $a These results support a contribution from neighbor-neighbor cooperative effects based on overlapping ion clouds of neighboring duplexes as described by the Schatz and Mirkin groups. To evaluate the extent of this contribution, a dual-duplex polymer-DNA system was developed where the melting of one type of duplex within a pre-formed aggregate did not lead to aggregate dissolution. Excitingly, the resulting sharp melting transitions for both duplexes supported a predominant contribution of neighbor-neighbor cooperativity to the sharpness of the melting transitions instead of a phase transition mechanism requiring aggregate dissolution.
590 $a School code: 0163.
650 4 $a Biophysics, General. $3 1019105
650 4 $a Chemistry, Biochemistry. $3 1017722
650 4 $a Chemistry, Polymer. $3 1018428
690 $a 0487
690 $a 0495
690 $a 0786
710 2 0 $a Northwestern University. $3 1018161
773 0 $t Dissertation Abstracts International $g 67-03B.
790 $a 0163
790 1 0 $a Nguyen, SonBinh T., $e advisor
791 $a Ph.D.
792 $a 2006
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3212832