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Application of bi-cell surface plasm...

Mani, Rinosh Joshua.

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Application of bi-cell surface plasmon resonance for the detection of aptamer mediated thrombin capture in serum.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Application of bi-cell surface plasmon resonance for the detection of aptamer mediated thrombin capture in serum./
Author:	Mani, Rinosh Joshua.
Description:	85 p.
Notes:	Adviser: Kenneth D. Clinkenbeard.
Contained By:	Masters Abstracts International44-06.
Subject:	Biology, Molecular. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1436226
ISBN:	9780542728846

Application of bi-cell surface plasmon resonance for the detection of aptamer mediated thrombin capture in serum.
Mani, Rinosh Joshua.

Application of bi-cell surface plasmon resonance for the detection of aptamer mediated thrombin capture in serum. - 85 p.

Adviser: Kenneth D. Clinkenbeard.

Thesis (M.S.)--Oklahoma State University, 2006.

Using the bi-cell surface plasmon resonance instrument, we were able to detect thrombin in the concentration range of 500 femtomoles to 2 picomoles with thrombin aptamer as the capture probe. The lowest limit of detection was 250 femtomoles. The affinity of the aptamer for prothrombin was also investigated, which was detected at 2 picomoles. Using serum samples, 1 picomole of thrombin was detected. We proved that an aptamer could be used as a capture ligand in bi-cell SPR instrument. This model could be used in a clinical setting for the real time detection of diagnostically relevant biomolecules.

ISBN: 9780542728846Subjects--Topical Terms:

1017719
Biology, Molecular.

Application of bi-cell surface plasmon resonance for the detection of aptamer mediated thrombin capture in serum.
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020 $a 9780542728846
035 $a (UMI)AAI1436226
035 $a AAI1436226
040 $a UMI $c UMI
100 1 $a Mani, Rinosh Joshua. $3 1291617
245 1 0 $a Application of bi-cell surface plasmon resonance for the detection of aptamer mediated thrombin capture in serum.
300 $a 85 p.
500 $a Adviser: Kenneth D. Clinkenbeard.
500 $a Source: Masters Abstracts International, Volume: 44-06, page: 2726.
502 $a Thesis (M.S.)--Oklahoma State University, 2006.
520 $a Using the bi-cell surface plasmon resonance instrument, we were able to detect thrombin in the concentration range of 500 femtomoles to 2 picomoles with thrombin aptamer as the capture probe. The lowest limit of detection was 250 femtomoles. The affinity of the aptamer for prothrombin was also investigated, which was detected at 2 picomoles. Using serum samples, 1 picomole of thrombin was detected. We proved that an aptamer could be used as a capture ligand in bi-cell SPR instrument. This model could be used in a clinical setting for the real time detection of diagnostically relevant biomolecules.
590 $a School code: 0664.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biophysics, General. $3 1019105
690 $a 0307
690 $a 0786
710 2 0 $a Oklahoma State University. $3 626630
773 0 $t Masters Abstracts International $g 44-06.
790 $a 0664
790 1 0 $a Clinkenbeard, Kenneth D., $e advisor
791 $a M.S.
792 $a 2006
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1436226