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Development of a single nucleotide p...

Zhang, Hai.

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Development of a single nucleotide polymorphism-based electronic DNA microarray technique for the detection and species differentiation of viable Campylobacter.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Development of a single nucleotide polymorphism-based electronic DNA microarray technique for the detection and species differentiation of viable Campylobacter./
Author:	Zhang, Hai.
Description:	135 p.
Notes:	Source: Masters Abstracts International, Volume: 44-06, page: 2784.
Contained By:	Masters Abstracts International44-06.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MR13919
ISBN:	9780494139196

Development of a single nucleotide polymorphism-based electronic DNA microarray technique for the detection and species differentiation of viable Campylobacter.
Zhang, Hai.

Development of a single nucleotide polymorphism-based electronic DNA microarray technique for the detection and species differentiation of viable Campylobacter. - 135 p.

Source: Masters Abstracts International, Volume: 44-06, page: 2784.

Thesis (M.Sc.)--University of Alberta (Canada), 2006.

Campylobacter jejuni, C. coli, and C. lari are the three major food-borne pathogenic Campylobacter species that cause the most frequent occurrences of acute bacterial gastroenteritis around the world. This thesis presents the successful development of a reverse transcription-polymerase chain reaction (RT-PCR) electronic DNA microarray approach for the simultaneous detection and species differentiation of viable Campylobacter. The mRNA of the 60-kDa heat shock protein gene ( hsp60) was used as the viability marker, and two closely located single nucleotide polymorphisms (SNPs) within this gene were chosen as the species marker. A 200-bp fragment amplified from the hsp60 mRNA by RT-PCR was detected with species-specific fluorescently-labeled reporters using an electronic DNA microarray technique.

ISBN: 9780494139196Subjects--Topical Terms:

1017734
Biology, Microbiology.

Development of a single nucleotide polymorphism-based electronic DNA microarray technique for the detection and species differentiation of viable Campylobacter.
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020 $a 9780494139196
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100 1 $a Zhang, Hai. $3 1291614
245 1 0 $a Development of a single nucleotide polymorphism-based electronic DNA microarray technique for the detection and species differentiation of viable Campylobacter.
300 $a 135 p.
500 $a Source: Masters Abstracts International, Volume: 44-06, page: 2784.
502 $a Thesis (M.Sc.)--University of Alberta (Canada), 2006.
520 $a Campylobacter jejuni, C. coli, and C. lari are the three major food-borne pathogenic Campylobacter species that cause the most frequent occurrences of acute bacterial gastroenteritis around the world. This thesis presents the successful development of a reverse transcription-polymerase chain reaction (RT-PCR) electronic DNA microarray approach for the simultaneous detection and species differentiation of viable Campylobacter. The mRNA of the 60-kDa heat shock protein gene ( hsp60) was used as the viability marker, and two closely located single nucleotide polymorphisms (SNPs) within this gene were chosen as the species marker. A 200-bp fragment amplified from the hsp60 mRNA by RT-PCR was detected with species-specific fluorescently-labeled reporters using an electronic DNA microarray technique.
520 $a This technique can detect as few as two viable Campylobacter cells, and will not detect dead cells. The evaluation of 14 blind Campylobacter samples showed 100% agreement with their identities, demonstrating its high specificity. This technique was preliminarily applied to six authentic chicken samples as well.
590 $a School code: 0351.
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Health Sciences, Public Health. $3 1017659
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710 2 0 $a University of Alberta (Canada). $3 626651
773 0 $t Masters Abstracts International $g 44-06.
790 $a 0351
791 $a M.Sc.
792 $a 2006
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MR13919