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Electric field gradient focusing-UV ...

Lin, Shu-Ling.

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Electric field gradient focusing-UV detection for protein analysis.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Electric field gradient focusing-UV detection for protein analysis./
作者:	Lin, Shu-Ling.
面頁冊數:	141 p.
附註:	Source: Dissertation Abstracts International, Volume: 67-04, Section: B, page: 1983.
Contained By:	Dissertation Abstracts International67-04B.
標題:	Chemistry, Analytical. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3214149
ISBN:	9780542649189

Electric field gradient focusing-UV detection for protein analysis.
Lin, Shu-Ling.

Electric field gradient focusing-UV detection for protein analysis. - 141 p.

Source: Dissertation Abstracts International, Volume: 67-04, Section: B, page: 1983.

Thesis (Ph.D.)--Brigham Young University, 2006.

Based on the experimental results described in this dissertation, EFGF shows potential for selective isolation, concentration, and quantitation of trace analytes such as proteins in biomedical samples.

ISBN: 9780542649189Subjects--Topical Terms:

586156
Chemistry, Analytical.

Electric field gradient focusing-UV detection for protein analysis.
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245 1 0 $a Electric field gradient focusing-UV detection for protein analysis.
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500 $a Source: Dissertation Abstracts International, Volume: 67-04, Section: B, page: 1983.
502 $a Thesis (Ph.D.)--Brigham Young University, 2006.
520 $a Based on the experimental results described in this dissertation, EFGF shows potential for selective isolation, concentration, and quantitation of trace analytes such as proteins in biomedical samples.
520 $a Electric field gradient focusing (EFGF) utilizes a hydrodynamic flow and an electric field gradient to focus and concentrate charged analytes and order them in a separation channel according to electrophoretic mobility. Elution can be achieved by decreasing the applied voltage or increasing the hydrodynamic flow. EFGF has the advantages of concentrating a large volume (100 muL) of target proteins without significant band broadening and simultaneously removing unwanted components from the sample. Two types of EFGF devices have been investigated to concentrate and separate proteins: a fiber-based EFGF device and a hydrogel-based EFGF device.
520 $a Using fiber-based EFGF with UV detection, a concentration factor as high as 15,000 and a concentration limit of detection as low as 30 pM were achieved using bovine serum albumin as a model protein. I also demonstrated the potential of using fiber-based EFGF for quantitative protein analysis. Simultaneous desalting and protein concentration as well as online concentration of ferritin and simultaneous removal of albumin from a sample matrix were also performed using this fiber-based EFGF system.
520 $a In the approach of utilizing hydrogel-based EFGF, online concentration of amyloglucosidase indicated a concentration limit of detection of approximately 20 ng/mL (200 pM) from a sample volume of 100 muL. Both voltage-controlled and flow-controlled elution methods were demonstrated using a 3-component protein mixture. Concentration of human alpha1-acid glycoprotein with simultaneous removal of human serum albumin was also described.
520 $a A tandem EFGF system, which integrates fiber-based and hydrogel-based EFGF sections, was also investigated to selectively concentrate and separate proteins in a mixture. By carefully controlling the voltages applied to both sections, charged analytes with high mobilities were trapped in the fiber-based section, analytes with intermediate mobilities in the hydrogel-based section, and analytes with low mobilities not at all. A 3-way switching valve was incorporated in the system to purge the analytes with high mobilities periodically. Selective concentration and separation of myoglobin from a mixture were performed using the tandem EFGF system.
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