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Development of bio-photonic sensor b...

Kim, Chan Kyu.

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Development of bio-photonic sensor based on laser-induced fluorescence.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Development of bio-photonic sensor based on laser-induced fluorescence./
作者:	Kim, Chan Kyu.
面頁冊數:	135 p.
附註:	Adviser: Jagdish P. Singh.
Contained By:	Dissertation Abstracts International69-01B.
標題:	Biophysics, General. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3296215
ISBN:	9780549410331

Development of bio-photonic sensor based on laser-induced fluorescence.
Kim, Chan Kyu.

Development of bio-photonic sensor based on laser-induced fluorescence. - 135 p.

Adviser: Jagdish P. Singh.

Thesis (Ph.D.)--Mississippi State University, 2007.

Key words. Laser induced fluorescence, human breast cancer cell lines, Azotobacter vinelandii, DNA, gold nanoparticles.

ISBN: 9780549410331Subjects--Topical Terms:

1019105
Biophysics, General.

Development of bio-photonic sensor based on laser-induced fluorescence.
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245 1 0 $a Development of bio-photonic sensor based on laser-induced fluorescence.
300 $a 135 p.
500 $a Adviser: Jagdish P. Singh.
500 $a Source: Dissertation Abstracts International, Volume: 69-01, Section: B, page: 0387.
502 $a Thesis (Ph.D.)--Mississippi State University, 2007.
520 $a Key words. Laser induced fluorescence, human breast cancer cell lines, Azotobacter vinelandii, DNA, gold nanoparticles.
520 $a Laser-induced fluorescence (LIF) has been shown to be potentially useful for identifying microorganisms in real time. It is a selective and sensitive technique because the excitation is performed at one wavelength while the emission is monitored at longer wavelengths so that background from the excitation source can be eliminated. This specialized optical property of LIF can be applied to development of an optical sensor capable of quickly, non-invasively, and quantitatively probing complex biochemical transformations in microorganisms. Various bio-photonic optical fiber sensors based on laser-induced fluorescence (LIF) spectroscopy were developed as diagnostic tools for microorganisms. In the first phase, the enhancement of the sensitivity and selectivity of the optical sensor system focused on diagnosis of human breast cancer cell lines and Azotobacter vinelandii (an aerobic soil-dwelling organism). Auto-fluorescence spectra from human breast cancer cell lines and Azotobacter vinelandii corresponding to different growth environments were investigated. Then, the study has expanded to include the use of gold nanoparticles for specific DNA detection. The use of gold nanoparticles opens a door into construction of a compact, highly specific, inexpensive and user-friendly optical fiber senor for specific DNA detection. An optical fiber laser-induced fluorescence (LIF) sensor based has been developed to detect single-strand (ss) DNA hybridization at the femtomolar level. Effects of various experimental parameters and configuration were investigated in order to optimize sensor performance and miniaturize sensor size.
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650 4 $a Physics, Molecular. $3 1018648
650 4 $a Physics, Optics. $3 1018756
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791 $a Ph.D.
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