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Exploring single-molecule interactio...

Wong, Wesley Philip.

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Exploring single-molecule interactions through 3D optical trapping and tracking: From thermal noise to protein refolding.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Exploring single-molecule interactions through 3D optical trapping and tracking: From thermal noise to protein refolding./
作者:	Wong, Wesley Philip.
面頁冊數:	167 p.
附註:	Advisers: David R. Nelson; Evan Evans.
Contained By:	Dissertation Abstracts International68-02B.
標題:	Biophysics, General. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3251327

Exploring single-molecule interactions through 3D optical trapping and tracking: From thermal noise to protein refolding.
Wong, Wesley Philip.

Exploring single-molecule interactions through 3D optical trapping and tracking: From thermal noise to protein refolding. - 167 p.

Advisers: David R. Nelson; Evan Evans.

Thesis (Ph.D.)--Harvard University, 2007.

The focus of this thesis is the development and application of a novel technique for investigating the structure and dynamics of weak interactions between and within single-molecules. This approach is designed to explore unusual features in bi-directional transitions near equilibrium. The basic idea is to infer molecular events by observing changes in the three-dimensional Brownian fluctuations of a functionalized microsphere held weakly near a reactive substrate. Experimentally, I have developed a unique optical tweezers system that combines an interference technique for accurate 3D tracking (&sim;1 nm vertically, and &sim;2-3 nm laterally) with a continuous autofocus system which stabilizes the trap height to within 1-2 mn over hours. A number of different physical and biological systems were investigated with this instrument. Data interpretation was assisted by a multi-scale Brownian Dynamics simulation that I have developed. I have explored the 3D signatures of different molecular tethers, distinguishing between single and multiple attachments, as well as between stiff and soft linkages. As well, I have developed a technique for measuring the force-dependent compliance of molecular tethers from thermal noise fluctuations and demonstrated this with a short ssDNA oligomer. Another practical approach that I have developed for extracting information from fluctuation measurements is Inverse Brownian Dynamics, which yields the underlying potential of mean force and position dependent diffusion coefficient from the Brownian motion of a particle. I have also developed a new force calibration method that takes into account video motion blur, and that uses this information to measure bead dynamics. Perhaps most significantly, I have trade the first direct observations of the refolding of spectrin repeats under mechanical force, and investigated the force-dependent kinetics of this transition.Subjects--Topical Terms:

1019105
Biophysics, General.

Exploring single-molecule interactions through 3D optical trapping and tracking: From thermal noise to protein refolding.
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245 1 0 $a Exploring single-molecule interactions through 3D optical trapping and tracking: From thermal noise to protein refolding.
300 $a 167 p.
500 $a Advisers: David R. Nelson; Evan Evans.
500 $a Source: Dissertation Abstracts International, Volume: 68-02, Section: B, page: 1044.
502 $a Thesis (Ph.D.)--Harvard University, 2007.
520 $a The focus of this thesis is the development and application of a novel technique for investigating the structure and dynamics of weak interactions between and within single-molecules. This approach is designed to explore unusual features in bi-directional transitions near equilibrium. The basic idea is to infer molecular events by observing changes in the three-dimensional Brownian fluctuations of a functionalized microsphere held weakly near a reactive substrate. Experimentally, I have developed a unique optical tweezers system that combines an interference technique for accurate 3D tracking (&sim;1 nm vertically, and &sim;2-3 nm laterally) with a continuous autofocus system which stabilizes the trap height to within 1-2 mn over hours. A number of different physical and biological systems were investigated with this instrument. Data interpretation was assisted by a multi-scale Brownian Dynamics simulation that I have developed. I have explored the 3D signatures of different molecular tethers, distinguishing between single and multiple attachments, as well as between stiff and soft linkages. As well, I have developed a technique for measuring the force-dependent compliance of molecular tethers from thermal noise fluctuations and demonstrated this with a short ssDNA oligomer. Another practical approach that I have developed for extracting information from fluctuation measurements is Inverse Brownian Dynamics, which yields the underlying potential of mean force and position dependent diffusion coefficient from the Brownian motion of a particle. I have also developed a new force calibration method that takes into account video motion blur, and that uses this information to measure bead dynamics. Perhaps most significantly, I have trade the first direct observations of the refolding of spectrin repeats under mechanical force, and investigated the force-dependent kinetics of this transition.
590 $a School code: 0084.
650 4 $a Biophysics, General. $3 1019105
650 4 $a Physics, Molecular. $3 1018648
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773 0 $t Dissertation Abstracts International $g 68-02B.
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790 1 0 $a Nelson, David R., $e advisor
791 $a Ph.D.
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