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MEMS thermal devices for biomolecula...

Wang, Li.

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MEMS thermal devices for biomolecular sensing and characterization.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	MEMS thermal devices for biomolecular sensing and characterization./
Author:	Wang, Li.
Description:	149 p.
Notes:	Source: Dissertation Abstracts International, Volume: 68-03, Section: B, page: 1896.
Contained By:	Dissertation Abstracts International68-03B.
Subject:	Engineering, Mechanical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3255972

MEMS thermal devices for biomolecular sensing and characterization.
Wang, Li.

MEMS thermal devices for biomolecular sensing and characterization. - 149 p.

Source: Dissertation Abstracts International, Volume: 68-03, Section: B, page: 1896.

Thesis (Ph.D.)--Carnegie Mellon University, 2007.

Thermal biosensing and calorimetry are universal methods for detecting and characterizing biological processes, almost all of which are thermally active. Miniaturization of these methods allows thermodynamic properties of biomolecules to be measured in controlled micro/nano environments with improved sensitivities as well as orders-of magnitude reduction in the consumption of biological material. The goal of this thesis is to develop miniaturized thermal biosensors and differential scanning calorimetric (DSC) sensors for measuring metabolic reactions as well as conformational changes and interactions of biomolecules, such as DNA and proteins, by using microelectromechanical systems (MEMS) technology.Subjects--Topical Terms:

783786
Engineering, Mechanical.

MEMS thermal devices for biomolecular sensing and characterization.
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100 1 $a Wang, Li. $3 1278373
245 1 0 $a MEMS thermal devices for biomolecular sensing and characterization.
300 $a 149 p.
500 $a Source: Dissertation Abstracts International, Volume: 68-03, Section: B, page: 1896.
502 $a Thesis (Ph.D.)--Carnegie Mellon University, 2007.
520 $a Thermal biosensing and calorimetry are universal methods for detecting and characterizing biological processes, almost all of which are thermally active. Miniaturization of these methods allows thermodynamic properties of biomolecules to be measured in controlled micro/nano environments with improved sensitivities as well as orders-of magnitude reduction in the consumption of biological material. The goal of this thesis is to develop miniaturized thermal biosensors and differential scanning calorimetric (DSC) sensors for measuring metabolic reactions as well as conformational changes and interactions of biomolecules, such as DNA and proteins, by using microelectromechanical systems (MEMS) technology.
520 $a A MEMS differential thermal biosensor fully integrated with microfluidics has been developed for metabolic monitoring applications in both flow-injection and flow-through modes. The differential device has two microfluidic chambers with resistive heaters integrated on the diaphragm of each chamber, as well as a thermopile with hot and cold junctions lying respectively on the diaphragms. The chambers have a small volume (1.2 mul) for minimized sample consumption and are respectively connected to the substrate by microchannels for easy handling of sample solutions. The device has a responsivity of 1.2 V/W and time constant of 0.6 s. Using enzyme-functionalized beads to interact with the metabolite solution, the biosensor has demonstrated a glucose concentration measurement resolution of 0.12 mM in flow-injection mode, and 0.48 mM in flow-through mode with a flow rate of 0.5 ml/h. The measurement resolution of the device has been analyzed and the results generally agree with the experimental data. Analytical and numerical models have been developed to afford in-depth understanding of the device physics.
520 $a Two generations of MEMS DSC devices are presented. The devices consist of a pair of freestanding microfluidic chambers integrated with MEMS thermal elements, and allow efficient handling and measurement of small volume of samples (&sim;1 mul). The first-generation MEMS DSC device uses a polysilicon-aluminum thermopile fabricated on silicon-nitride diaphragms, and has been applied to measuring the unfolding of the protein lysozyme at a concentration of 300 mg/ml. The necessity of this relatively high concentration is a consequence of the significant intrinsic noise in the polysilicon. To address this issue, the second-generation MEMS DSC device utilizes a nickel-chromium bimetallic thermopile, which offers much reduced intrinsic noise. Additionally, the diaphragms are fabricated from a polymer using inexpensive, low-temperature processes. The second-generation device has shown a sensitivity improvement by an order of magnitude, and has allowed measurement of protein unfolding at much reduced concentrations (< 20 mg/ml). Thermodynamic properties of the proteins have been computed and found to agree reasonably with the values given in the literature. Numerical simulations of the second-generation device have also been performed, and the results correctly predict the trend in the measurement data. A discussion is also given to possible directions for future research toward developing MEMS DSC devices with further improved performance that will ultimately allow highly sensitive, label-free characterization of biomolecular transitions and interactions.
590 $a School code: 0041.
650 4 $a Engineering, Mechanical. $3 783786
690 $a 0548
710 2 $a Carnegie Mellon University. $3 1018096
773 0 $t Dissertation Abstracts International $g 68-03B.
790 $a 0041
791 $a Ph.D.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3255972