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Roles of vacuolar sorting receptor p...

Wang, Junqi.

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Roles of vacuolar sorting receptor proteins and prevacuolar compartments in mung bean seeds.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Roles of vacuolar sorting receptor proteins and prevacuolar compartments in mung bean seeds./
Author:	Wang, Junqi.
Description:	131 p.
Notes:	Adviser: Jiang Liwen.
Contained By:	Dissertation Abstracts International69-01B.
Subject:	Biology, Botany. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3295088
ISBN:	9780549401728

Roles of vacuolar sorting receptor proteins and prevacuolar compartments in mung bean seeds.
Wang, Junqi.

Roles of vacuolar sorting receptor proteins and prevacuolar compartments in mung bean seeds. - 131 p.

Adviser: Jiang Liwen.

Thesis (Ph.D.)--The Chinese University of Hong Kong (Hong Kong), 2007.

Plants accumulate and store proteins in protein storage vacuoles (PSVs) during seed development and maturation. Upon seed germination, these storage proteins are mobilized to provide nutrients for seedling growth. However, little is known about the molecular mechanisms of protein degradation during seed germination and post-germination. Here I test the hypothesis that vacuolar sorting receptor (VSR) proteins play a role in mediating protein degradation in germinating and post-germination seeds. It is demonstrated that both VSR proteins and hydrolytic enzymes are synthesized de novo during mung bean seed germination and post-germination. Immunogold electron microscopy (EM) with VSR antibodies demonstrates that VSRs mainly locate to the peripheral membrane of multivesicular bodies (MVBs), presumably as recycling receptors in Day-1 germinating seeds, but become internalized to the MVB lumen, presumably for degradation at Day-3 post-germination. Chemical cross-linking and immunoprecipitation with VSR antibodies have identified the cysteine protease aleurain as a specific VSR-interacting protein in germinating and post-germination seeds. Further immunogold EM studies demonstrate that VSR and aleurain colocalize to MVBs, as well as PSVs in germinating and post-germination seeds. Thus, MVBs in germinating and post-germination seeds exercise dual functions: as a storage compartment for proteases that are physically separated from PSVs in the mature seed, and as an intermediate compartment for VSR-mediated delivery of proteases from the Golgi apparatus to the PSV for protein degradation during seed germination and post-germination.

ISBN: 9780549401728Subjects--Topical Terms:

1017825
Biology, Botany.

Roles of vacuolar sorting receptor proteins and prevacuolar compartments in mung bean seeds.
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020 $a 9780549401728
035 $a (UMI)AAI3295088
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100 1 $a Wang, Junqi. $3 1277314
245 1 0 $a Roles of vacuolar sorting receptor proteins and prevacuolar compartments in mung bean seeds.
300 $a 131 p.
500 $a Adviser: Jiang Liwen.
500 $a Source: Dissertation Abstracts International, Volume: 69-01, Section: B, page: 0052.
502 $a Thesis (Ph.D.)--The Chinese University of Hong Kong (Hong Kong), 2007.
520 $a Plants accumulate and store proteins in protein storage vacuoles (PSVs) during seed development and maturation. Upon seed germination, these storage proteins are mobilized to provide nutrients for seedling growth. However, little is known about the molecular mechanisms of protein degradation during seed germination and post-germination. Here I test the hypothesis that vacuolar sorting receptor (VSR) proteins play a role in mediating protein degradation in germinating and post-germination seeds. It is demonstrated that both VSR proteins and hydrolytic enzymes are synthesized de novo during mung bean seed germination and post-germination. Immunogold electron microscopy (EM) with VSR antibodies demonstrates that VSRs mainly locate to the peripheral membrane of multivesicular bodies (MVBs), presumably as recycling receptors in Day-1 germinating seeds, but become internalized to the MVB lumen, presumably for degradation at Day-3 post-germination. Chemical cross-linking and immunoprecipitation with VSR antibodies have identified the cysteine protease aleurain as a specific VSR-interacting protein in germinating and post-germination seeds. Further immunogold EM studies demonstrate that VSR and aleurain colocalize to MVBs, as well as PSVs in germinating and post-germination seeds. Thus, MVBs in germinating and post-germination seeds exercise dual functions: as a storage compartment for proteases that are physically separated from PSVs in the mature seed, and as an intermediate compartment for VSR-mediated delivery of proteases from the Golgi apparatus to the PSV for protein degradation during seed germination and post-germination.
520 $a Storage proteins synthesized during seed development are transported to PSVs for storage. However, relatively little is known about the mechanisms of storage protein transport. A putative VSR-interacting protein termed S2 was identified as mung bean 8S globulin. Thus, I test the hypothesis that VSR proteins may be involved in storage protein transport to PSVs in developing mung bean seeds. Immunogold EM with 52 (8S globulin) antibody demonstrates that transport of 8S globulin to PSVs is Golgi-mediated, involving dense vesicle (DV) and a novel prevacuolar compartment (PVC). The novel PVC consists of storage protein aggregates and small internal vesicles. Immunogold EM with S2 (8S globulin) antibody demonstrates that MVBs contain 8S globulin at early stage of seed development. Further immunogold EM studies demonstrate that VSR and 8S globulin colocalize to DVs and the novel PVCs. In vitro binding study demonstrates that calcium ion can stabilize interaction between VSRs and 8S globulin. Thus, VSR proteins may mediate storage protein transport to PSVs via a novel PVC.
590 $a School code: 1307.
650 4 $a Biology, Botany. $3 1017825
650 4 $a Biology, Cell. $3 1017686
650 4 $a Biology, Plant Physiology. $3 1017865
690 $a 0309
690 $a 0379
690 $a 0817
710 2 $a The Chinese University of Hong Kong (Hong Kong). $3 1017547
773 0 $t Dissertation Abstracts International $g 69-01B.
790 $a 1307
790 1 0 $a Liwen, Jiang, $e advisor
791 $a Ph.D.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3295088