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Microbial community analysis of the ...

Duque-Alarcon, Angela Patricia.

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Microbial community analysis of the octocoral Eunicea fusca: Combined use of cultivation-dependent and independent techniques.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Microbial community analysis of the octocoral Eunicea fusca: Combined use of cultivation-dependent and independent techniques./
Author:	Duque-Alarcon, Angela Patricia.
Description:	80 p.
Notes:	Adviser: Russell Kerr.
Contained By:	Masters Abstracts International45-04.
Subject:	Agriculture, Fisheries and Aquaculture. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1441541

Microbial community analysis of the octocoral Eunicea fusca: Combined use of cultivation-dependent and independent techniques.
Duque-Alarcon, Angela Patricia.

Microbial community analysis of the octocoral Eunicea fusca: Combined use of cultivation-dependent and independent techniques. - 80 p.

Adviser: Russell Kerr.

Thesis (M.S.)--Florida Atlantic University, 2007.

The octocoral Eunicea fusca is the source of important anti-inflammatory compounds such as the diterpenes fuscol and the fuscosides. Evidence suggests that these compounds are being produce by bacterial sources that live in symbiosis with the coral. As part of an investigation to better understand the role of bacteria associated with E. fusca, the characterization of the bacterial community using two different techniques (culture dependent technique and culture independent technique) and sea water samples was done. Sea water samples were used as a control to determine how closely associated bacteria are to this octocoral. The polymerase chain reaction (PCR) with universal bacterial primers FC27 and RC1492 specific to prokaryotic 16S rDNA gene sequences was used to characterize the total bacterial population when using both the culturable and the non-culturable approaches. The results indicate a diverse group of bacteria associated to E. fusca composed of 10 different groups. The proteobacteria group was the most predominant group when both techniques were used. The alpha-proteobacteria represented the highest percentage of bacteria associated to E. fusca .Subjects--Topical Terms:

1020913
Agriculture, Fisheries and Aquaculture.

Microbial community analysis of the octocoral Eunicea fusca: Combined use of cultivation-dependent and independent techniques.
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035 $a (UMI)AAI1441541
035 $a AAI1441541
040 $a UMI $c UMI
100 1 $a Duque-Alarcon, Angela Patricia. $3 1272590
245 1 0 $a Microbial community analysis of the octocoral Eunicea fusca: Combined use of cultivation-dependent and independent techniques.
300 $a 80 p.
500 $a Adviser: Russell Kerr.
500 $a Source: Masters Abstracts International, Volume: 45-04, page: 1873.
502 $a Thesis (M.S.)--Florida Atlantic University, 2007.
520 $a The octocoral Eunicea fusca is the source of important anti-inflammatory compounds such as the diterpenes fuscol and the fuscosides. Evidence suggests that these compounds are being produce by bacterial sources that live in symbiosis with the coral. As part of an investigation to better understand the role of bacteria associated with E. fusca, the characterization of the bacterial community using two different techniques (culture dependent technique and culture independent technique) and sea water samples was done. Sea water samples were used as a control to determine how closely associated bacteria are to this octocoral. The polymerase chain reaction (PCR) with universal bacterial primers FC27 and RC1492 specific to prokaryotic 16S rDNA gene sequences was used to characterize the total bacterial population when using both the culturable and the non-culturable approaches. The results indicate a diverse group of bacteria associated to E. fusca composed of 10 different groups. The proteobacteria group was the most predominant group when both techniques were used. The alpha-proteobacteria represented the highest percentage of bacteria associated to E. fusca .
590 $a School code: 0119.
650 4 $a Agriculture, Fisheries and Aquaculture. $3 1020913
650 4 $a Biology, Ecology. $3 1017726
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Oceanography. $3 783691
690 $a 0307
690 $a 0329
690 $a 0416
690 $a 0792
710 2 $a Florida Atlantic University. $3 1017837
773 0 $t Masters Abstracts International $g 45-04.
790 $a 0119
790 1 0 $a Kerr, Russell, $e advisor
791 $a M.S.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1441541