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Development of genomic tools in Bras...

Iniguez-Luy, Federico L.

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Development of genomic tools in Brassica rapa and Brassica oleracea and their use to study the evolutionary fate of redundant genes.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Development of genomic tools in Brassica rapa and Brassica oleracea and their use to study the evolutionary fate of redundant genes./
作者:	Iniguez-Luy, Federico L.
面頁冊數:	363 p.
附註:	Adviser: Richard M. Amasino.
Contained By:	Dissertation Abstracts International68-08B.
標題:	Agriculture, Plant Culture. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3278890
ISBN:	9780549197348

Development of genomic tools in Brassica rapa and Brassica oleracea and their use to study the evolutionary fate of redundant genes.
Iniguez-Luy, Federico L.

Development of genomic tools in Brassica rapa and Brassica oleracea and their use to study the evolutionary fate of redundant genes. - 363 p.

Adviser: Richard M. Amasino.

Thesis (Ph.D.)--The University of Wisconsin - Madison, 2007.

Brassica rapa L. and Brassica oleracea L. are members of the Brassicaceae with extensive morphological diversity including commercial vegetables oilseeds, condiments and forages. The ability to inter-cross morphotypes within B. rapa and B. oleracea has made them suitable organisms to conduct detailed genetic studies including comparative mapping and QTL analysis. These studies, however, have been disconnected from one another due to the lack of public resources. In this study, we developed public resources for Brassica genomics that aim to integrate findings from different disciplines within the Brassica research community. We developed 587 public simple sequence repeats (SSR) molecular markers from B. oleracea sequence information. In addition, we generated public, self-compatible, rapid cycling and immortal populations for B. rapa and B. oleracea. We utilized these populations, SSR markers and sequenced tagged restriction fragment length polymorphism (RFLP) markers to construct genetic linkage maps. Sequence information from markers in the B. rapa and B. oleracea maps were used to align these two genomes with the A. thaliana genome. We found putatively linked SSR markers to B. oleracea mutant phenotypes. Putative map positions of such mutants can serve as the starting point to identify candidate genes based on comparative analysis with A. thaliana.

ISBN: 9780549197348Subjects--Topical Terms:

1018669
Agriculture, Plant Culture.

Development of genomic tools in Brassica rapa and Brassica oleracea and their use to study the evolutionary fate of redundant genes.
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245 1 0 $a Development of genomic tools in Brassica rapa and Brassica oleracea and their use to study the evolutionary fate of redundant genes.
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520 $a Brassica rapa L. and Brassica oleracea L. are members of the Brassicaceae with extensive morphological diversity including commercial vegetables oilseeds, condiments and forages. The ability to inter-cross morphotypes within B. rapa and B. oleracea has made them suitable organisms to conduct detailed genetic studies including comparative mapping and QTL analysis. These studies, however, have been disconnected from one another due to the lack of public resources. In this study, we developed public resources for Brassica genomics that aim to integrate findings from different disciplines within the Brassica research community. We developed 587 public simple sequence repeats (SSR) molecular markers from B. oleracea sequence information. In addition, we generated public, self-compatible, rapid cycling and immortal populations for B. rapa and B. oleracea. We utilized these populations, SSR markers and sequenced tagged restriction fragment length polymorphism (RFLP) markers to construct genetic linkage maps. Sequence information from markers in the B. rapa and B. oleracea maps were used to align these two genomes with the A. thaliana genome. We found putatively linked SSR markers to B. oleracea mutant phenotypes. Putative map positions of such mutants can serve as the starting point to identify candidate genes based on comparative analysis with A. thaliana.
520 $a Our mapping results and others have highlighted the redundant nature of the Brassica genomes. In recent years, an ancient polyploid origin has been discovered for several members of the Brassicaceae lineage, including diploid Brassica crops and A. thaliana . In this study, seven sets of replicated genes in a 2.2Mb triplicated region of the B. oleracea genome were investigated for relative gene expression in 9 inbred lines representing 6 diverse morphotypes. Our results showed divergence of gene expression patterns of redundant genes retained after the triplication event that shaped the lineages of Brassica crops. The progressive divergence in gene expression (quantitative and spatial divergence) could indicate that duplicated and triplicated genes retained over a period 18 MY may be evolving towards subfunctionalization.
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