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Differential gene expression of muri...

Myer, Sarah E.

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Differential gene expression of murine blastocyst stage embryos and murine embryonic stem cells.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Differential gene expression of murine blastocyst stage embryos and murine embryonic stem cells./
Author:	Myer, Sarah E.
Description:	168 p.
Notes:	Adviser: Lee F. Rickords.
Contained By:	Dissertation Abstracts International69-02B.
Subject:	Biology, Genetics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3302330
ISBN:	9780549478898

Differential gene expression of murine blastocyst stage embryos and murine embryonic stem cells.
Myer, Sarah E.

Differential gene expression of murine blastocyst stage embryos and murine embryonic stem cells. - 168 p.

Adviser: Lee F. Rickords.

Thesis (Ph.D.)--Oklahoma State University, 2007.

Scope and method of study. Embryonic stem cells (ESC), derived from cultured blastocyst embryos, have the potential to provide insight into developmental and medical issues. The cellular transition, however, from blastocyst to ESC has not been well characterized. The purpose of this study was to identify transcriptional differences between blastocysts and ESC through use of suppression subtraction hybridization (SSH) in murine CD1 blastocyst and 129SvEv ESC. Comparisons between the following groups: blastocyst - blastocyst, trophectoderm - blastocyst, blastocyst - ESC, and ESC - fibroblast feeder cell, resulted in the cloning, sequencing, and identification of uniquely expressed sequences. The second objective was to compare expression levels of three selected transcripts through real time PCR in blastocysts, 129SvEv ESC, C57BL/6 ESC, and fibroblast feeder cells.

ISBN: 9780549478898Subjects--Topical Terms:

1017730
Biology, Genetics.

Differential gene expression of murine blastocyst stage embryos and murine embryonic stem cells.
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020 $a 9780549478898
035 $a (UMI)AAI3302330
035 $a AAI3302330
040 $a UMI $c UMI
100 1 $a Myer, Sarah E. $3 1271780
245 1 0 $a Differential gene expression of murine blastocyst stage embryos and murine embryonic stem cells.
300 $a 168 p.
500 $a Adviser: Lee F. Rickords.
500 $a Source: Dissertation Abstracts International, Volume: 69-02, Section: B, page: 0839.
502 $a Thesis (Ph.D.)--Oklahoma State University, 2007.
520 $a Scope and method of study. Embryonic stem cells (ESC), derived from cultured blastocyst embryos, have the potential to provide insight into developmental and medical issues. The cellular transition, however, from blastocyst to ESC has not been well characterized. The purpose of this study was to identify transcriptional differences between blastocysts and ESC through use of suppression subtraction hybridization (SSH) in murine CD1 blastocyst and 129SvEv ESC. Comparisons between the following groups: blastocyst - blastocyst, trophectoderm - blastocyst, blastocyst - ESC, and ESC - fibroblast feeder cell, resulted in the cloning, sequencing, and identification of uniquely expressed sequences. The second objective was to compare expression levels of three selected transcripts through real time PCR in blastocysts, 129SvEv ESC, C57BL/6 ESC, and fibroblast feeder cells.
520 $a Findings and conclusions. Using SSH, 33 unique clones were identified and 10 had strong ribosomal homologies while 23 had either gene specific homologies or else were unknown. Prdx1, Ywahz, and Vdacs3 were analyzed through real time PCR. For Prdx1, fibroblast feeder cells had the lowest expression level, blastocysts had a 4.81 fold increase, 129SvEv ESC had a 7.51 fold increase, and the C57BL/6 ESC showed a 27.28 fold increase. Expression levels were significantly different between the blastocyst and C57BL/6 samples as well as the fibroblast feeder cells and both ESC samples based on DeltaCT statistical analysis. For Ywhaz, the fibroblast feeder sample showed the lowest expression level, blastocysts had a 7.65 fold increase, 129SvEv ESC showed a 6.49 fold increase, and C57BL/6 ESC showed a 16.56 fold difference. The two ESC values were significantly different as was the fibroblast feeder cell value from all other samples. For Vdacs3, the blastocyst sample had the lowest level of expression, fibroblast feeder cells had a 2.16 fold difference, 129SvEv ESC had a 68.07 fold difference, and C57BL/6 ESC had a 161.04 fold difference. Statistically, the blastocyst sample was different from both of the ESC samples and the fibroblast sample from either ESC sample. Prdx1 and Vdacs3 both demonstrated significant upregulation in comparison between blastocyst stage embryos and ESC and function to enhance cell survival and continued cell reproduction.
590 $a School code: 0664.
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Molecular. $3 1017719
690 $a 0307
690 $a 0369
710 2 0 $a Oklahoma State University. $b Biochemistry & Molecular Biology. $3 1271781
773 0 $t Dissertation Abstracts International $g 69-02B.
790 $a 0664
790 1 0 $a Allen, Robert W. $e committee member
790 1 0 $a Jarolim, Kirby L. $e committee member
790 1 0 $a Malayer, Jerry R. $e committee member
790 1 0 $a Rickords, Lee F., $e advisor
791 $a Ph.D.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3302330