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Alkaline digestion of head and pubic...

Soltysiak, Shannon A.

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Alkaline digestion of head and pubic hairs for nuclear and mitochondrial DNA analysis.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Alkaline digestion of head and pubic hairs for nuclear and mitochondrial DNA analysis./
Author:	Soltysiak, Shannon A.
Description:	80 p.
Notes:	Adviser: David R. Foran.
Contained By:	Masters Abstracts International45-06.
Subject:	Anthropology, Medical and Forensic. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1444301
ISBN:	9780549021506

Alkaline digestion of head and pubic hairs for nuclear and mitochondrial DNA analysis.
Soltysiak, Shannon A.

Alkaline digestion of head and pubic hairs for nuclear and mitochondrial DNA analysis. - 80 p.

Adviser: David R. Foran.

Thesis (M.S.)--Michigan State University, 2007.

Hair is a common form of evidence found at crime scenes, and may be the sole trace evidence to tie a suspect or victim to a location or crime. Isolating DNA from head or pubic hairs is an attractive means of placing a suspect and/or victim at a crime scene. Nuclear DNA is thought to be degraded or not present in shed (telogen) hairs, making STR analysis using commercially available kits difficult or impossible. MtDNA analysis of hair shafts is often successful, but many labs have not validated the method, and in the end, it is not an absolute identifier. Likewise, DNA isolation from hair shafts involves laborious extraction techniques, which can increase the likelihood of contamination. An alternative to standard DNA isolation from hair shafts is alkaline extraction, in which keratin from hair is hydrolyzed but DNA is kept intact. This method was used to extract DNA from head and pubic hair shafts. Hairs were washed in an enzymatic detergent, and then rinsed with ethanol and water. The hairs were then incubated in concentrated sodium hydroxide until completely dissolved, neutralized, and DNA eluted in TE on a spin column. A 220bp product of mtDNA was obtainable from 68% of alkaline digested head hairs and from 98% of alkaline digested pubic hairs. Hair DNA that successfully generated mtDNA product was tested on single and multi-copy nuclear markers. Multi-copy markers show promise as possible sex determinates, while small single copy loci have real-time PCR applications.

ISBN: 9780549021506Subjects--Topical Terms:

1020279
Anthropology, Medical and Forensic.

Alkaline digestion of head and pubic hairs for nuclear and mitochondrial DNA analysis.
LDR:02382nam 2200289 a 45 001 945988
005 20110523
008 110523s2007 ||||||||||||||||| ||eng d
020 $a 9780549021506
035 $a (UMI)AAI1444301
035 $a AAI1444301
040 $a UMI $c UMI
100 1 $a Soltysiak, Shannon A. $3 1269397
245 1 0 $a Alkaline digestion of head and pubic hairs for nuclear and mitochondrial DNA analysis.
300 $a 80 p.
500 $a Adviser: David R. Foran.
500 $a Source: Masters Abstracts International, Volume: 45-06, page: 2884.
502 $a Thesis (M.S.)--Michigan State University, 2007.
520 $a Hair is a common form of evidence found at crime scenes, and may be the sole trace evidence to tie a suspect or victim to a location or crime. Isolating DNA from head or pubic hairs is an attractive means of placing a suspect and/or victim at a crime scene. Nuclear DNA is thought to be degraded or not present in shed (telogen) hairs, making STR analysis using commercially available kits difficult or impossible. MtDNA analysis of hair shafts is often successful, but many labs have not validated the method, and in the end, it is not an absolute identifier. Likewise, DNA isolation from hair shafts involves laborious extraction techniques, which can increase the likelihood of contamination. An alternative to standard DNA isolation from hair shafts is alkaline extraction, in which keratin from hair is hydrolyzed but DNA is kept intact. This method was used to extract DNA from head and pubic hair shafts. Hairs were washed in an enzymatic detergent, and then rinsed with ethanol and water. The hairs were then incubated in concentrated sodium hydroxide until completely dissolved, neutralized, and DNA eluted in TE on a spin column. A 220bp product of mtDNA was obtainable from 68% of alkaline digested head hairs and from 98% of alkaline digested pubic hairs. Hair DNA that successfully generated mtDNA product was tested on single and multi-copy nuclear markers. Multi-copy markers show promise as possible sex determinates, while small single copy loci have real-time PCR applications.
590 $a School code: 0128.
650 4 $a Anthropology, Medical and Forensic. $3 1020279
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Sociology, Criminology and Penology. $3 1017569
690 $a 0307
690 $a 0339
690 $a 0627
710 2 $a Michigan State University. $3 676168
773 0 $t Masters Abstracts International $g 45-06.
790 $a 0128
790 1 0 $a Foran, David R., $e advisor
791 $a M.S.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1444301