東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Genetic and biochemical studies of c...

Field, Christine Marie.

Linked to FindBook

Google Book

Amazon

博客來

Genetic and biochemical studies of cytokinesis.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Genetic and biochemical studies of cytokinesis./
Author:	Field, Christine Marie.
Description:	180 p.
Notes:	Adviser: Marc Kirschner.
Contained By:	Dissertation Abstracts International68-05B.
Subject:	Biology, Cell. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3264950
ISBN:	9780549035701

Genetic and biochemical studies of cytokinesis.
Field, Christine Marie.

Genetic and biochemical studies of cytokinesis. - 180 p.

Adviser: Marc Kirschner.

Thesis (Ph.D.)--Harvard University, 2007.

Cytokinesis is the last step in the cell cycle, in which the cell physically separates into two daughters. It is a fundamental biological process of medical importance since successful cytokinesis is critical for the maintenance of genome stability. Cytokinesis requires coordinated activities of the cytoskeleton, membrane trafficking systems and cell cycle engine that are precisely controlled in space and time. We need to understand the mechanisms underlying each of these diverse processes and how they are coordinated. I used three approaches to try to understand cytokinesis mechanism.

ISBN: 9780549035701Subjects--Topical Terms:

1017686
Biology, Cell.

Genetic and biochemical studies of cytokinesis.
LDR:03354nam 2200313 a 45 001 945919
005 20110523
008 110523s2007 ||||||||||||||||| ||eng d
020 $a 9780549035701
035 $a (UMI)AAI3264950
035 $a AAI3264950
040 $a UMI $c UMI
100 1 $a Field, Christine Marie. $3 1269326
245 1 0 $a Genetic and biochemical studies of cytokinesis.
300 $a 180 p.
500 $a Adviser: Marc Kirschner.
500 $a Source: Dissertation Abstracts International, Volume: 68-05, Section: B, page: 2757.
502 $a Thesis (Ph.D.)--Harvard University, 2007.
520 $a Cytokinesis is the last step in the cell cycle, in which the cell physically separates into two daughters. It is a fundamental biological process of medical importance since successful cytokinesis is critical for the maintenance of genome stability. Cytokinesis requires coordinated activities of the cytoskeleton, membrane trafficking systems and cell cycle engine that are precisely controlled in space and time. We need to understand the mechanisms underlying each of these diverse processes and how they are coordinated. I used three approaches to try to understand cytokinesis mechanism.
520 $a We performed parallel chemical genetic and genome-wide RNA interference screens in Drosophila tissue culture cells, looking for genes encoding proteins required for cytokinesis and small molecules that target them. We identified 50 small molecule inhibitors and 214 important genes. Most proteins whose knockdown caused strong inhibition were already known, but we did identify a new subunit of the Aurora B kinase complex. Our weaker hits include important cytokinesis proteins likely required at other times in the cell cycle, including 12 genes encoding vesicle transport proteins. We also identified 54 genes predicted to encode proteins of unknown function.
520 $a I characterized a series of mutations in the anillin gene, a strong hit in our RNAi screen. I found defects in cytokinesis, cellularization and pole cell formation. Mutations that result in amino acid changes in Anillin's C-terminal PH domain caused defects in septin recruitment to the cellularization front and contractile ring during zygotic cell divisions, perturbing both processes. Our data indicate an important role for Anillin in scaffolding cleavage furrow proteins, directly stabilizing intracellular bridges, and indirectly stabilizing newly deposited plasma membrane during cellularization.
520 $a Cell-free systems allow researchers to analyze biological processes in isolation, setting the stage for inhibition and fractionation of the underlying biochemistry. I explored whether cell cycle-regulated Xenopus extracts might provide a cell-free system to study aspects of cytokinesis. Upon comparing mitotic and interphase extracts, I found they exhibited very different actomyosin dynamics. My exploration on a molecular level revealed cell cycle differences in actin filament nucleation. This has led me to propose novel hypotheses concerning regulation of actin nucleation during the cell cycle.
590 $a School code: 0084.
650 4 $a Biology, Cell. $3 1017686
650 4 $a Biology, Genetics. $3 1017730
690 $a 0369
690 $a 0379
710 2 $a Harvard University. $3 528741
773 0 $t Dissertation Abstracts International $g 68-05B.
790 $a 0084
790 1 0 $a Kirschner, Marc, $e advisor
791 $a Ph.D.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3264950