東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Cell-mediated immune response to por...

Charerntantanakul, Wasin.

Linked to FindBook

Google Book

Amazon

博客來

Cell-mediated immune response to porcine reproductive and respiratory syndrome virus.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Cell-mediated immune response to porcine reproductive and respiratory syndrome virus./
Author:	Charerntantanakul, Wasin.
Description:	142 p.
Notes:	Adviser: James A. Roth.
Contained By:	Dissertation Abstracts International67-08B.
Subject:	Agriculture, Animal Pathology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3229057
ISBN:	9780542831478

Cell-mediated immune response to porcine reproductive and respiratory syndrome virus.
Charerntantanakul, Wasin.

Cell-mediated immune response to porcine reproductive and respiratory syndrome virus. - 142 p.

Adviser: James A. Roth.

Thesis (Ph.D.)--Iowa State University, 2006.

The cell-mediated immune (CMI) response of pigs to porcine reproductive and respiratory syndrome virus (PRRSV) is low in magnitude and appears late after infection. The ability of PRRSV to suppress CMI response is not known. We reported in this dissertation that PRRSV has the ability to significantly suppress CD25, interferon gamma (IFNgamma), and tumor-necrosis factor alpha expression by T cells in response to concanavalin A and phorbol 12-myristate 13-acetate plus ionomycin, respectively. The suppressive ability of PRRSV associated with PRRSV virulence and type of myeloid antigen-presenting cells the virus infect. Virulent PRRSV significantly suppressed T cell response, whereas attenuated PRRSV did not. Monocytes supported T cell suppression more effectively than monocyte-derived macrophages and immature monocyte-derived dendritic cells. T cell suppression negatively associated with increased interleukin-10 (IL-10) gene expression. Neutralization of IL-10 activity by anti-swine IL-10 monoclonal antibodies inhibited T cell suppression.

ISBN: 9780542831478Subjects--Topical Terms:

1021764
Agriculture, Animal Pathology.

Cell-mediated immune response to porcine reproductive and respiratory syndrome virus.
LDR:03465nam 2200301 a 45 001 945822
005 20110523
008 110523s2006 ||||||||||||||||| ||eng d
020 $a 9780542831478
035 $a (UMI)AAI3229057
035 $a AAI3229057
040 $a UMI $c UMI
100 1 $a Charerntantanakul, Wasin. $3 1269234
245 1 0 $a Cell-mediated immune response to porcine reproductive and respiratory syndrome virus.
300 $a 142 p.
500 $a Adviser: James A. Roth.
500 $a Source: Dissertation Abstracts International, Volume: 67-08, Section: B, page: 4289.
502 $a Thesis (Ph.D.)--Iowa State University, 2006.
520 $a The cell-mediated immune (CMI) response of pigs to porcine reproductive and respiratory syndrome virus (PRRSV) is low in magnitude and appears late after infection. The ability of PRRSV to suppress CMI response is not known. We reported in this dissertation that PRRSV has the ability to significantly suppress CD25, interferon gamma (IFNgamma), and tumor-necrosis factor alpha expression by T cells in response to concanavalin A and phorbol 12-myristate 13-acetate plus ionomycin, respectively. The suppressive ability of PRRSV associated with PRRSV virulence and type of myeloid antigen-presenting cells the virus infect. Virulent PRRSV significantly suppressed T cell response, whereas attenuated PRRSV did not. Monocytes supported T cell suppression more effectively than monocyte-derived macrophages and immature monocyte-derived dendritic cells. T cell suppression negatively associated with increased interleukin-10 (IL-10) gene expression. Neutralization of IL-10 activity by anti-swine IL-10 monoclonal antibodies inhibited T cell suppression.
520 $a PRRSV modified-live Virus (MLV) vaccine is currently used in the field to control diseases caused by PRRSV. To enhance CMI response to PRRSV MLV vaccine, five different vaccine adjuvants (bacterial endotoxin-derived adjuvant, mixed open reading frame 5 (ORF5) peptides derived from 5 PRRSV isolates, porcine IFNalpha, polyinosinic-polycytidylic acid stabilized with polylysine and carboxymethylcellulose, and porcine IL-12) were studied. Administration of mixed ORF5 peptides at 14 and 28 days after PRRS MLV vaccination significantly increased IFNgamma production in CD4-CD8+gammadelta +, CD4-CD8-gammadelta+, and CD4-CD8+gammadelta- T cells. Administration of porcine IL-12 at 1 day after PRRS MLV vaccination significantly increased IFNgamma production in CD4+CD8 +gammadelta-, CD4-CD8+gammadelta +, and CD4-CD8+gammadelta - T cells. Significantly increased IFNgamma expression in CD4 +CD8+gammadelta-, CD4-CD8 +gammadelta+, CD4-CD8+gammadelta - T cells but not CD4-CD8-gammadelta + T cells were correlated significantly with the reduction of lung lesion scores and viremia after virulent PRRSV challenge. Administration of porcine IFNalpha at -1, 0, and 1 day and porcine IL,-12 at 1 day after PRRS MLV vaccination significantly increased CD25 expression in CD4-CD8 +gammadelta+ T cells. However, the increased CD25 expression did not correlate with protection. None of the vaccine adjuvants contributed to the reduction of lung lesion scores and viremia in comparison to PRRS MLV alone.
590 $a School code: 0097.
650 4 $a Agriculture, Animal Pathology. $3 1021764
650 4 $a Biology, Veterinary Science. $3 1021733
650 4 $a Biology, Virology. $3 1019068
690 $a 0476
690 $a 0720
690 $a 0778
710 2 $a Iowa State University. $3 1017855
773 0 $t Dissertation Abstracts International $g 67-08B.
790 $a 0097
790 1 0 $a Roth, James A., $e advisor
791 $a Ph.D.
792 $a 2006
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3229057