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The recovery and characterization of...

Linville, Jason Graef.

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The recovery and characterization of vertebrate DNA from forensically important fly larvae: An optimization study.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	The recovery and characterization of vertebrate DNA from forensically important fly larvae: An optimization study./
Author:	Linville, Jason Graef.
Description:	123 p.
Notes:	Chair: Jeffrey D. Wells.
Contained By:	Dissertation Abstracts International64-08B.
Subject:	Biology, Entomology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3101520
ISBN:	9780496491070

The recovery and characterization of vertebrate DNA from forensically important fly larvae: An optimization study.
Linville, Jason Graef.

The recovery and characterization of vertebrate DNA from forensically important fly larvae: An optimization study. - 123 p.

Chair: Jeffrey D. Wells.

Thesis (Ph.D.)--The University of Alabama at Birmingham, 2003.

Entomological evidence is commonly collected during a death investigation. Insects, such as flies and their larvae (maggots), are most frequently used to help determine the time of death. However, as the field of forensic entomology advances, new techniques are being incorporated into the field. One such technique is using DNA analysis of a maggot's gut contents to determine what the maggot has been feeding on. This information may be used to help identify a missing body, identify maggots that have moved onto a corpse from another food source, or resolve a chain of evidence dispute. A maggot's crop, a food storage organ at the anterior end of the gut, is an ideal target for recovering relatively undigested DNA from the maggot's food source. Although mitochondrial DNA sequence analysis from 3rd larval instar crop contents has previously been reported, many questions remain about the practical limits of this analysis, and these questions form the basis for this dissertation research. Mitochondrial DNA and short tandem repeat (STR) analyses were attempted on maggots of different ages, from 1-day-old maggots to postfeeding maggots. DNA analysis was attempted on maggots that had been kept alive off the food source for 24 h. A wash method using a bleach solution was developed to remove external contamination from maggots before crop content analysis. The effects that common forensic entomological preservation methods have on DNA analysis of maggot crop contents were evaluated. Maggots were analyzed after feeding on a secondary food source to determine whether the primary food source could be detected in the crop. Finally, two non-sequencing methods were developed for determining the vertebrate species of maggot crop extractions, which also contained invertebrate DNA.

ISBN: 9780496491070Subjects--Topical Terms:

1018619
Biology, Entomology.

The recovery and characterization of vertebrate DNA from forensically important fly larvae: An optimization study.
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100 1 $a Linville, Jason Graef. $3 1269160
245 1 4 $a The recovery and characterization of vertebrate DNA from forensically important fly larvae: An optimization study.
300 $a 123 p.
500 $a Chair: Jeffrey D. Wells.
500 $a Source: Dissertation Abstracts International, Volume: 64-08, Section: B, page: 3641.
502 $a Thesis (Ph.D.)--The University of Alabama at Birmingham, 2003.
520 $a Entomological evidence is commonly collected during a death investigation. Insects, such as flies and their larvae (maggots), are most frequently used to help determine the time of death. However, as the field of forensic entomology advances, new techniques are being incorporated into the field. One such technique is using DNA analysis of a maggot's gut contents to determine what the maggot has been feeding on. This information may be used to help identify a missing body, identify maggots that have moved onto a corpse from another food source, or resolve a chain of evidence dispute. A maggot's crop, a food storage organ at the anterior end of the gut, is an ideal target for recovering relatively undigested DNA from the maggot's food source. Although mitochondrial DNA sequence analysis from 3rd larval instar crop contents has previously been reported, many questions remain about the practical limits of this analysis, and these questions form the basis for this dissertation research. Mitochondrial DNA and short tandem repeat (STR) analyses were attempted on maggots of different ages, from 1-day-old maggots to postfeeding maggots. DNA analysis was attempted on maggots that had been kept alive off the food source for 24 h. A wash method using a bleach solution was developed to remove external contamination from maggots before crop content analysis. The effects that common forensic entomological preservation methods have on DNA analysis of maggot crop contents were evaluated. Maggots were analyzed after feeding on a secondary food source to determine whether the primary food source could be detected in the crop. Finally, two non-sequencing methods were developed for determining the vertebrate species of maggot crop extractions, which also contained invertebrate DNA.
590 $a School code: 0005.
650 4 $a Biology, Entomology. $3 1018619
650 4 $a Biology, Molecular. $3 1017719
690 $a 0307
690 $a 0353
710 2 $a The University of Alabama at Birmingham. $3 1019443
773 0 $t Dissertation Abstracts International $g 64-08B.
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790 1 0 $a Wells, Jeffrey D., $e advisor
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