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Biochemical analysis of cell divisio...

Kotun, Allen M.

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Biochemical analysis of cell division protein complexes in Streptomyces coelicolor.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Biochemical analysis of cell division protein complexes in Streptomyces coelicolor./
作者:	Kotun, Allen M.
面頁冊數:	110 p.
附註:	Adviser: Joseph R. McCormick.
Contained By:	Masters Abstracts International46-03.
標題:	Biology, Genetics. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1449201
ISBN:	9780549287155

Biochemical analysis of cell division protein complexes in Streptomyces coelicolor.
Kotun, Allen M.

Biochemical analysis of cell division protein complexes in Streptomyces coelicolor. - 110 p.

Adviser: Joseph R. McCormick.

Thesis (M.S.)--Duquesne University, 2007.

In Streptomyces coelicolor, at least six proteins are involved in the process of cell division: FtsZ, FtsQ, FtsL, DivIC, FtsW, and FtsI. The purpose of this study was to biochemically analyze putative cell division protein interactions to complement and possibly expand upon previous genetic studies. To accomplish this, S. coelicolor strains expressing epitope-tagged cell division proteins were created and pilot experiments using a Ni-NTA pull down assay were performed. His8-FtsZ was found to be stable in vivo; however, many fusion proteins were found to be nonfunctional. The Ni-NTA pull down assay worked well to isolate His8-FtsZ, but other fusion proteins could not be visualized by western blot analysis due to low levels of expression. The antibodies used in western blot analyses were found to be problematic due to high background and nonreactivity. Attempts to optimize these assays were performed; however, further optimization is needed to continue this study.

ISBN: 9780549287155Subjects--Topical Terms:

1017730
Biology, Genetics.

Biochemical analysis of cell division protein complexes in Streptomyces coelicolor.
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520 $a In Streptomyces coelicolor, at least six proteins are involved in the process of cell division: FtsZ, FtsQ, FtsL, DivIC, FtsW, and FtsI. The purpose of this study was to biochemically analyze putative cell division protein interactions to complement and possibly expand upon previous genetic studies. To accomplish this, S. coelicolor strains expressing epitope-tagged cell division proteins were created and pilot experiments using a Ni-NTA pull down assay were performed. His8-FtsZ was found to be stable in vivo; however, many fusion proteins were found to be nonfunctional. The Ni-NTA pull down assay worked well to isolate His8-FtsZ, but other fusion proteins could not be visualized by western blot analysis due to low levels of expression. The antibodies used in western blot analyses were found to be problematic due to high background and nonreactivity. Attempts to optimize these assays were performed; however, further optimization is needed to continue this study.
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