東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Nanopore analysis of nucleic acids.

Butler, Thomas.

Linked to FindBook

Google Book

Amazon

博客來

Nanopore analysis of nucleic acids.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Nanopore analysis of nucleic acids./
Author:	Butler, Thomas.
Description:	106 p.
Notes:	Adviser: Jens H. Gundlach.
Contained By:	Dissertation Abstracts International68-05B.
Subject:	Biophysics, General. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3265304
ISBN:	9780549038917

Nanopore analysis of nucleic acids.
Butler, Thomas.

Nanopore analysis of nucleic acids. - 106 p.

Adviser: Jens H. Gundlach.

Thesis (Ph.D.)--University of Washington, 2007.

Nanopore analysis of nucleic acids is a novel technique to study the physical properties of nucleic acids at the single-molecule level. It also has the potential to be a central component of a fundamentally new DNA sequencing methodology. In this technique, individual single-stranded DNA or RNA molecules are detected and characterized by an ionic current blockade produced when they are electrophoretically driven through a nanometer-scale pore. The primary focus of this thesis is a detailed characterization and interpretation of the sub-level structure of the ionic current blockades produced during the interaction between single-stranded DNA and RNA molecules and the Staphylococcus aureus alpha-Hemolysin protein nanopore. These investigations reveal important mechanistic details of the interaction between nucleic acids and alpha-Hemolysin. The results presented in this thesis contribute significantly to the growing utility of nanopore analysis as a model system to study biologically relevant physical and chemical processes, and they may help with the development of novel, nanopore-based technological applications. A secondary focus of this thesis is the initial steps toward genetically engineering Mycobacterium smegmatis porin A as an alternative protein nanopore for nucleic acid analysis. Successful creation of an additional protein nanopore represents a substantial scientific and technological breakthrough.

ISBN: 9780549038917Subjects--Topical Terms:

1019105
Biophysics, General.

Nanopore analysis of nucleic acids.
LDR:02255nam 2200265 a 45 001 940427
005 20110518
008 110518s2007 ||||||||||||||||| ||eng d
020 $a 9780549038917
035 $a (UMI)AAI3265304
035 $a AAI3265304
040 $a UMI $c UMI
100 1 $a Butler, Thomas. $3 1264555
245 1 0 $a Nanopore analysis of nucleic acids.
300 $a 106 p.
500 $a Adviser: Jens H. Gundlach.
500 $a Source: Dissertation Abstracts International, Volume: 68-05, Section: B, page: 2889.
502 $a Thesis (Ph.D.)--University of Washington, 2007.
520 $a Nanopore analysis of nucleic acids is a novel technique to study the physical properties of nucleic acids at the single-molecule level. It also has the potential to be a central component of a fundamentally new DNA sequencing methodology. In this technique, individual single-stranded DNA or RNA molecules are detected and characterized by an ionic current blockade produced when they are electrophoretically driven through a nanometer-scale pore. The primary focus of this thesis is a detailed characterization and interpretation of the sub-level structure of the ionic current blockades produced during the interaction between single-stranded DNA and RNA molecules and the Staphylococcus aureus alpha-Hemolysin protein nanopore. These investigations reveal important mechanistic details of the interaction between nucleic acids and alpha-Hemolysin. The results presented in this thesis contribute significantly to the growing utility of nanopore analysis as a model system to study biologically relevant physical and chemical processes, and they may help with the development of novel, nanopore-based technological applications. A secondary focus of this thesis is the initial steps toward genetically engineering Mycobacterium smegmatis porin A as an alternative protein nanopore for nucleic acid analysis. Successful creation of an additional protein nanopore represents a substantial scientific and technological breakthrough.
590 $a School code: 0250.
650 4 $a Biophysics, General. $3 1019105
690 $a 0786
710 2 $a University of Washington. $3 545923
773 0 $t Dissertation Abstracts International $g 68-05B.
790 $a 0250
790 1 0 $a Gundlach, Jens H., $e advisor
791 $a Ph.D.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3265304