語系:
繁體中文
English
說明(常見問題)
回圖書館首頁
手機版館藏查詢
登入
回首頁
切換:
標籤
|
MARC模式
|
ISBD
Study of seed germination-associated...
~
Liu, Po-Pu.
FindBook
Google Book
Amazon
博客來
Study of seed germination-associated genes using Arabidopsis enhancer-trap.
紀錄類型:
書目-語言資料,印刷品 : Monograph/item
正題名/作者:
Study of seed germination-associated genes using Arabidopsis enhancer-trap./
作者:
Liu, Po-Pu.
面頁冊數:
148 p.
附註:
Adviser: Hiroyuki Nonogaki.
Contained By:
Dissertation Abstracts International68-06B.
標題:
Biology, Botany. -
電子資源:
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3268293
ISBN:
9780549072768
Study of seed germination-associated genes using Arabidopsis enhancer-trap.
Liu, Po-Pu.
Study of seed germination-associated genes using Arabidopsis enhancer-trap.
- 148 p.
Adviser: Hiroyuki Nonogaki.
Thesis (Ph.D.)--Oregon State University, 2007.
In mature Arabidopsis seeds, the testa (seed coat) is no longer a living tissue. Thus, major sites of gene expression in imbibed seeds are the internal living tissues such as the embryo and the endosperm. To elucidate the molecular mechanisms of seed germination, the gene regulation in these two tissues during germination needs to be investigated. An enhancer-trap approach was used in this study to identify tissue- and stage-specific gene expression in seeds. The Arabidopsis enhancer-trap lines (Thomas Jack's population, CS31086) from the Arabidopsis Biological Resource Center (ABRC) were screened for beta-glucuronidase (GUS) expression in imbibed seeds in order to identify and characterize seed germination-associated genes. One hundred twenty one independent lines exhibiting diverse tissue-specific GUS expression patterns were isolated and kept as the Seed-GUS-Expression enhancer-trap library. This library was donated to the ABRC (stock no. CS24362--CS24480), and is now available to the international seed research community. Ninety one lines showed GUS expression predominantly in the micropylar end of the seed (named BME [Blue Micropylar End] lines), indicating that the micropylar region of Arabidopsis seed is selectively activated during germination. One of these lines, BME3, had a T-DNA insertion site in the 5' upstream region of a GATA-type zinc finger transcription factor gene (termed BME3-ZF). The BME3-ZF mRNA accumulated in seeds during cold stratification, suggesting its involvement in the physiological changes that accelerate the release of dormancy. BME3-ZF was expressed just prior to the expression of two GA biosynthesis genes, AtGA20ox3 and AtGA3ox1 which are also induced by cold stratification. The BME3-ZF knockout plants produced seeds exhibiting increased dormancy, which showed reduced response to cold stratification. The ungerminated knockout seeds exhibited testa rupture, but failed to penetrate the endosperm layer. Application of gibberellic acid (GA3) rescued impaired germination of the knockout seeds without cold stratification, indicating that the normal GA signal transduction pathway is present in the knockout seeds. These results indicate BME3 GATA zinc finger protein is a positive regulator of Arabidopsis seed germination. In this study, we provide the proof-of-concept study for the Seed-GUS-Expression enhancer trap library and the detailed procedures to use a combination of gene-expression analysis of wild-type seeds and functional analysis using knockout plants for encouraging international collaborations to utilize this library which is a useful tool to identify the genes critical for seed germination.
ISBN: 9780549072768Subjects--Topical Terms:
1017825
Biology, Botany.
Study of seed germination-associated genes using Arabidopsis enhancer-trap.
LDR
:03584nam 2200289 a 45
001
939615
005
20110517
008
110517s2007 ||||||||||||||||| ||eng d
020
$a
9780549072768
035
$a
(UMI)AAI3268293
035
$a
AAI3268293
040
$a
UMI
$c
UMI
100
1
$a
Liu, Po-Pu.
$3
1263718
245
1 0
$a
Study of seed germination-associated genes using Arabidopsis enhancer-trap.
300
$a
148 p.
500
$a
Adviser: Hiroyuki Nonogaki.
500
$a
Source: Dissertation Abstracts International, Volume: 68-06, Section: B, page: 3567.
502
$a
Thesis (Ph.D.)--Oregon State University, 2007.
520
$a
In mature Arabidopsis seeds, the testa (seed coat) is no longer a living tissue. Thus, major sites of gene expression in imbibed seeds are the internal living tissues such as the embryo and the endosperm. To elucidate the molecular mechanisms of seed germination, the gene regulation in these two tissues during germination needs to be investigated. An enhancer-trap approach was used in this study to identify tissue- and stage-specific gene expression in seeds. The Arabidopsis enhancer-trap lines (Thomas Jack's population, CS31086) from the Arabidopsis Biological Resource Center (ABRC) were screened for beta-glucuronidase (GUS) expression in imbibed seeds in order to identify and characterize seed germination-associated genes. One hundred twenty one independent lines exhibiting diverse tissue-specific GUS expression patterns were isolated and kept as the Seed-GUS-Expression enhancer-trap library. This library was donated to the ABRC (stock no. CS24362--CS24480), and is now available to the international seed research community. Ninety one lines showed GUS expression predominantly in the micropylar end of the seed (named BME [Blue Micropylar End] lines), indicating that the micropylar region of Arabidopsis seed is selectively activated during germination. One of these lines, BME3, had a T-DNA insertion site in the 5' upstream region of a GATA-type zinc finger transcription factor gene (termed BME3-ZF). The BME3-ZF mRNA accumulated in seeds during cold stratification, suggesting its involvement in the physiological changes that accelerate the release of dormancy. BME3-ZF was expressed just prior to the expression of two GA biosynthesis genes, AtGA20ox3 and AtGA3ox1 which are also induced by cold stratification. The BME3-ZF knockout plants produced seeds exhibiting increased dormancy, which showed reduced response to cold stratification. The ungerminated knockout seeds exhibited testa rupture, but failed to penetrate the endosperm layer. Application of gibberellic acid (GA3) rescued impaired germination of the knockout seeds without cold stratification, indicating that the normal GA signal transduction pathway is present in the knockout seeds. These results indicate BME3 GATA zinc finger protein is a positive regulator of Arabidopsis seed germination. In this study, we provide the proof-of-concept study for the Seed-GUS-Expression enhancer trap library and the detailed procedures to use a combination of gene-expression analysis of wild-type seeds and functional analysis using knockout plants for encouraging international collaborations to utilize this library which is a useful tool to identify the genes critical for seed germination.
590
$a
School code: 0172.
650
4
$a
Biology, Botany.
$3
1017825
650
4
$a
Biology, Molecular.
$3
1017719
650
4
$a
Biology, Plant Physiology.
$3
1017865
690
$a
0307
690
$a
0309
690
$a
0817
710
2
$a
Oregon State University.
$3
625720
773
0
$t
Dissertation Abstracts International
$g
68-06B.
790
$a
0172
790
1 0
$a
Nonogaki, Hiroyuki,
$e
advisor
791
$a
Ph.D.
792
$a
2007
856
4 0
$u
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3268293
筆 0 讀者評論
館藏地:
全部
電子資源
出版年:
卷號:
館藏
1 筆 • 頁數 1 •
1
條碼號
典藏地名稱
館藏流通類別
資料類型
索書號
使用類型
借閱狀態
預約狀態
備註欄
附件
W9109601
電子資源
11.線上閱覽_V
電子書
EB W9109601
一般使用(Normal)
在架
0
1 筆 • 頁數 1 •
1
多媒體
評論
新增評論
分享你的心得
Export
取書館
處理中
...
變更密碼
登入