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The effect of radiation and repeated...

Burrell, Cheryl G.

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The effect of radiation and repeated sub-culturing on TGF-beta1 signaling in FRTL-5 cells.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	The effect of radiation and repeated sub-culturing on TGF-beta1 signaling in FRTL-5 cells./
作者:	Burrell, Cheryl G.
面頁冊數:	143 p.
附註:	Adviser: Lora Green.
Contained By:	Dissertation Abstracts International68-04B.
標題:	Biology, Cell. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3257608

The effect of radiation and repeated sub-culturing on TGF-beta1 signaling in FRTL-5 cells.
Burrell, Cheryl G.

The effect of radiation and repeated sub-culturing on TGF-beta1 signaling in FRTL-5 cells. - 143 p.

Adviser: Lora Green.

Thesis (Ph.D.)--Loma Linda University, 2007.

From our ongoing in vitro studies using the Fisher Rat Thyroid cell line-5 (FRTL-5) we recorded accelerated growth, reduced follicularization and reduction in thyroxin release that occurred as the cells were repeatedly sub-cultured. We also recorded that these changes occurred earlier and more rapidly following radiation exposure. We determined that TGF-beta1 production increased under both conditions. We hypothesized that alteration in the TGF-beta1 signaling pathway contributed to the changes observed in the cellular properties of FRTL-5 cells. Our objective was to examine some of the players in the TGF-beta1 signaling pathway to determine whether radiation and/or repeated sub-culturing promoted changes in their levels and/or activity.Subjects--Topical Terms:

1017686
Biology, Cell.

The effect of radiation and repeated sub-culturing on TGF-beta1 signaling in FRTL-5 cells.
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035 $a (UMI)AAI3257608
035 $a AAI3257608
040 $a UMI $c UMI
100 1 $a Burrell, Cheryl G. $3 1263256
245 1 4 $a The effect of radiation and repeated sub-culturing on TGF-beta1 signaling in FRTL-5 cells.
300 $a 143 p.
500 $a Adviser: Lora Green.
500 $a Source: Dissertation Abstracts International, Volume: 68-04, Section: B, page: 2390.
502 $a Thesis (Ph.D.)--Loma Linda University, 2007.
520 $a From our ongoing in vitro studies using the Fisher Rat Thyroid cell line-5 (FRTL-5) we recorded accelerated growth, reduced follicularization and reduction in thyroxin release that occurred as the cells were repeatedly sub-cultured. We also recorded that these changes occurred earlier and more rapidly following radiation exposure. We determined that TGF-beta1 production increased under both conditions. We hypothesized that alteration in the TGF-beta1 signaling pathway contributed to the changes observed in the cellular properties of FRTL-5 cells. Our objective was to examine some of the players in the TGF-beta1 signaling pathway to determine whether radiation and/or repeated sub-culturing promoted changes in their levels and/or activity.
520 $a We quantified changes in cellular growth rate using the MTS cell proliferation assay. TGF-beta1 ligand and receptor levels were quantified via ELISA, immunocytochemistry and western blot analysis respectively. The levels and activity of Smads 2, 3, 4 and 7, downstream effectors in the TGF-beta1 signaling pathway were also measured via western blotting. Lastly, we examined whether TGF-beta1 was correctly regulating the expression of its response genes; cyclin A and plasminogen activator inhibitor-1 (PAI-1) under our experimental conditions. To determine this we used luciferase reporter constructs containing promoters for cyclin A and PAI introduced to the cultures by transfection. PAI-1 production in response to exogenously added TGF-beta was also tested using a PAI-1 specific ELISA.
520 $a We observed an acceleration of growth that occurred earlier in irradiated cells than it did in cells subjected to repeat sub-culturing (p < 0.05). The TGF-beta1 receptor levels remained unchanged as a result of radiation and continual passage. We, however, observed decreases in the TGF-beta1 induced phosphorylation levels of Smads2 (p < 0.05) and 3 (p < 0.05) after radiation and repeated subculturing. However, no differences in the inherent un-activated levels of Smads2, 3, 4 and 7 were observed. Alterations were observed in TGF-beta1 ability to control the expression of cyclin A and PAI-1.
520 $a Collectively, these results support that alterations in the TGF-beta1 signaling pathway were contributing to the changes in cellular properties that we measured in our cell line, FRTL-5. These alterations were evident at the level of Smad signaling and transcription initiation.
590 $a School code: 0106.
650 4 $a Biology, Cell. $3 1017686
650 4 $a Chemistry, Radiation. $3 1017804
690 $a 0379
690 $a 0754
710 2 $a Loma Linda University. $3 1020166
773 0 $t Dissertation Abstracts International $g 68-04B.
790 $a 0106
790 1 0 $a Green, Lora, $e advisor
791 $a Ph.D.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3257608