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DNA mediated assembly of nanostructu...

Park, So-Jung.

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DNA mediated assembly of nanostructured materials: Structure, properties, and biodetection applications.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	DNA mediated assembly of nanostructured materials: Structure, properties, and biodetection applications./
Author:	Park, So-Jung.
Description:	165 p.
Notes:	Adviser: Chad A. Mirkin.
Contained By:	Dissertation Abstracts International63-11B.
Subject:	Chemistry, Inorganic. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3071685
ISBN:	0493916954

DNA mediated assembly of nanostructured materials: Structure, properties, and biodetection applications.
Park, So-Jung.

DNA mediated assembly of nanostructured materials: Structure, properties, and biodetection applications. - 165 p.

Adviser: Chad A. Mirkin.

Thesis (Ph.D.)--Northwestern University, 2002.

This dissertation describes a series of experiments aimed at determining the structures and properties of DNA-linked nanoparticle assemblies, and it explores ways of applying such materials in biological detection. Three general studies were undertaken. Synchrotron small angle x-ray scattering methods were used to probe the three dimensional superstructure of gold nanoparticle assemblies formed from different sized particles and different length DNA interconnects in various environments. Importantly, this study confirmed our hypothesis that nanoparticle assemblies with tailorable interparticle distances could be formed with DNA interconnects in aqueous solution. In contrast, in the condensed state, the assemblies show much shorter and linker-length independent particle spacings. As a result, dried aggregates exhibit semiconducting behavior with relatively small activation energies. These data suggested that one could use nanoparticles functionalized with oligonucleotides and DNA's molecular recognition properties for electronic applications.

ISBN: 0493916954Subjects--Topical Terms:

517253
Chemistry, Inorganic.

DNA mediated assembly of nanostructured materials: Structure, properties, and biodetection applications.
LDR:03583nam 2200289 a 45 001 937828
005 20110511
008 110511s2002 eng d
020 $a 0493916954
035 $a (UnM)AAI3071685
035 $a AAI3071685
040 $a UnM $c UnM
100 1 $a Park, So-Jung. $3 1261684
245 1 0 $a DNA mediated assembly of nanostructured materials: Structure, properties, and biodetection applications.
300 $a 165 p.
500 $a Adviser: Chad A. Mirkin.
500 $a Source: Dissertation Abstracts International, Volume: 63-11, Section: B, page: 5238.
502 $a Thesis (Ph.D.)--Northwestern University, 2002.
520 $a This dissertation describes a series of experiments aimed at determining the structures and properties of DNA-linked nanoparticle assemblies, and it explores ways of applying such materials in biological detection. Three general studies were undertaken. Synchrotron small angle x-ray scattering methods were used to probe the three dimensional superstructure of gold nanoparticle assemblies formed from different sized particles and different length DNA interconnects in various environments. Importantly, this study confirmed our hypothesis that nanoparticle assemblies with tailorable interparticle distances could be formed with DNA interconnects in aqueous solution. In contrast, in the condensed state, the assemblies show much shorter and linker-length independent particle spacings. As a result, dried aggregates exhibit semiconducting behavior with relatively small activation energies. These data suggested that one could use nanoparticles functionalized with oligonucleotides and DNA's molecular recognition properties for electronic applications.
520 $a Based on this observation, a DNA array detection method was developed in which the binding of Au nanoparticles functionalized with oligonucleotides led to conductivity changes associated with target-probe binding events. The binding events localize gold nanoparticles in an electrode gap; silver deposition facilitated by these nanoparticles bridges the gap and leads to readily measurable conductivity changes. Using this method, we have detected target DNA at concentrations as low as 500 fM with a point mutation selectivity factor of &sim;100,000:1. These unprecedented capabilities point towards a way of eliminating the need for polymerase chain reaction (PCR) in DNA detection.
520 $a Finally, we extended our strategy of DNA-based assembly to include oligonucleotide-modified streptavidin and block copolymers as the nanometer-sized building blocks. These structures have provided valuable insight into the fundamental properties of such hybrid structures and why they exhibit such unusual melting behaviors. Finally, DNA block copolymers with two distinct redox potentials were exploited in a novel electrochemical DNA detection format to detect single base mismatches. Significantly, these organic- and bio-nanostructures exhibit DNA hybridization properties that are similar to oligonucleotide-modified gold nanoparticles in that they have sharper melting profiles and larger binding constants for complementary oligonucleotides than molecular-probe functionalized DNA. These discoveries underscore the importance of developing the chemistry for nanoscale hybrid materials functionalized with biomolecules.
590 $a School code: 0163.
650 4 $a Chemistry, Inorganic. $3 517253
690 $a 0488
710 2 0 $a Northwestern University. $3 1018161
773 0 $t Dissertation Abstracts International $g 63-11B.
790 $a 0163
790 1 0 $a Mirkin, Chad A., $e advisor
791 $a Ph.D.
792 $a 2002
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3071685