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Development, characterization, and a...

Bullard, Katherine Marie.

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Development, characterization, and application of an integrated system for DNA separations.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Development, characterization, and application of an integrated system for DNA separations./
作者:	Bullard, Katherine Marie.
面頁冊數:	162 p.
附註:	Adviser: Andrew G. Ewing.
Contained By:	Dissertation Abstracts International59-08B.
標題:	Biology, Molecular. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9900994
ISBN:	0591978709

Development, characterization, and application of an integrated system for DNA separations.
Bullard, Katherine Marie.

Development, characterization, and application of an integrated system for DNA separations. - 162 p.

Adviser: Andrew G. Ewing.

Thesis (Ph.D.)--The Pennsylvania State University, 1998.

Traditionally, the technology for most biological separations has involved several labor intensive, time consuming steps. These methods, used in the conventional laboratory, are counter to today's emphasis on reducing cost and reagent volume as well as increasing simplicity and reliability. The development of new multifunctional, microfabricated chip devices addresses many of these problems while reducing sample volume and integrating all the aspects of an analysis on a single chip. Several of these devices, fabricated by microlithography, combine the advantages of capillary electrophoresis such as speed and small sample volume with ease of automation and high throughput. This is a combination that can satisfy the needs of many clinical applications and significant advances have recently been made towards applying this technology in the biomedical field.

ISBN: 0591978709Subjects--Topical Terms:

1017719
Biology, Molecular.

Development, characterization, and application of an integrated system for DNA separations.
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100 1 $a Bullard, Katherine Marie. $3 1261642
245 1 0 $a Development, characterization, and application of an integrated system for DNA separations.
300 $a 162 p.
500 $a Adviser: Andrew G. Ewing.
500 $a Source: Dissertation Abstracts International, Volume: 59-08, Section: B, page: 4068.
502 $a Thesis (Ph.D.)--The Pennsylvania State University, 1998.
520 $a Traditionally, the technology for most biological separations has involved several labor intensive, time consuming steps. These methods, used in the conventional laboratory, are counter to today's emphasis on reducing cost and reagent volume as well as increasing simplicity and reliability. The development of new multifunctional, microfabricated chip devices addresses many of these problems while reducing sample volume and integrating all the aspects of an analysis on a single chip. Several of these devices, fabricated by microlithography, combine the advantages of capillary electrophoresis such as speed and small sample volume with ease of automation and high throughput. This is a combination that can satisfy the needs of many clinical applications and significant advances have recently been made towards applying this technology in the biomedical field.
520 $a Despite the benefits described to date for the chip technology, there are still some challenges that complicate its use. Chief among these is sample introduction. This thesis discusses the development, characterization and application of a system that overcomes this and many other problems associated with these technologies. The use of continuous sample introduction and separation with a single capillary coupled to electrophoresis in narrow channels will be presented. This method utilizes a capillary to sample analytes and then deposit these analytes along the entrance to a wide channel for electrophoretic separation. A stepper motor is used to translate the capillary outlet along the channel entrance. An applied potential results in electrophoretic transfer of analytes from the capillary into the channel. At the exit end of the channel a spatially sensitive detector is used to detect the separated analytes. The channel system that will be discussed has been adapted to carry out DNA separations by filling the channel system with a polyacrylamide gel or sieving matrix. Experiments and data demonstrating the applicability of this system to carry out multiple DNA separations will be presented. Characterization of this new technology, paying particular attention to minimizing lateral dispersion of samples and application of this technology to a "real world" problem will be demonstrated. Lastly, future directions and adaptations of this technology will be discussed.
590 $a School code: 0176.
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650 4 $a Chemistry, Analytical. $3 586156
650 4 $a Chemistry, Biochemistry. $3 1017722
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