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Human tissue development in three-di...

Li, Yan.

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Human tissue development in three-dimensional fibrous matrix with defined microstructure.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Human tissue development in three-dimensional fibrous matrix with defined microstructure./
作者:	Li, Yan.
面頁冊數:	316 p.
附註:	Adviser: Shang-Tian Yang.
Contained By:	Dissertation Abstracts International63-01B.
標題:	Engineering, Biomedical. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3039497
ISBN:	0493528342

Human tissue development in three-dimensional fibrous matrix with defined microstructure.
Li, Yan.

Human tissue development in three-dimensional fibrous matrix with defined microstructure. - 316 p.

Adviser: Shang-Tian Yang.

Thesis (Ph.D.)--The Ohio State University, 2002.

Non-woven fibrous matrices provide large surface areas and three-dimensional environment that are conducive for cell growth and tissue development. The effects of micro-pore structure in non-woven PET matrix as a tissue scaffold for growing human placental trophoblast cells were studied. By controlling temperature, pressure and duration of thermal compression process, the porosity and pore size were precisely controlled. More uniform pore size distribution was obtained as quantified by liquid extrusion method. The viscoelastic behavior was observed, characterized by a distinct apparent modulus change in glass transition temperature region. A depth filtration method was developed as a new seeding process, which gave a significantly higher initial seeding density, more uniform cell distribution, and a higher final cell density compared to static seeding. A depth-filtration model was developed to simulate the seeding process and predict the maximum initial seeding density. It was found that low-porosity matrix supported more spreading cells and promoted proliferation. In contrast, high-porosity matrix supported more aggregated cells and enhanced the differentiated function. Also, 3-D system supported continuous tissue development, while 2-D system could not sustain long-term development with the loss of cells. The results suggested that the intimate spatial cell-cell and cell-matrix interactions controlled by the 3-D pore structure are essential for normal tissue development and function.

ISBN: 0493528342Subjects--Topical Terms:

1017684
Engineering, Biomedical.

Human tissue development in three-dimensional fibrous matrix with defined microstructure.
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245 1 0 $a Human tissue development in three-dimensional fibrous matrix with defined microstructure.
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500 $a Adviser: Shang-Tian Yang.
500 $a Source: Dissertation Abstracts International, Volume: 63-01, Section: B, page: 0390.
502 $a Thesis (Ph.D.)--The Ohio State University, 2002.
520 $a Non-woven fibrous matrices provide large surface areas and three-dimensional environment that are conducive for cell growth and tissue development. The effects of micro-pore structure in non-woven PET matrix as a tissue scaffold for growing human placental trophoblast cells were studied. By controlling temperature, pressure and duration of thermal compression process, the porosity and pore size were precisely controlled. More uniform pore size distribution was obtained as quantified by liquid extrusion method. The viscoelastic behavior was observed, characterized by a distinct apparent modulus change in glass transition temperature region. A depth filtration method was developed as a new seeding process, which gave a significantly higher initial seeding density, more uniform cell distribution, and a higher final cell density compared to static seeding. A depth-filtration model was developed to simulate the seeding process and predict the maximum initial seeding density. It was found that low-porosity matrix supported more spreading cells and promoted proliferation. In contrast, high-porosity matrix supported more aggregated cells and enhanced the differentiated function. Also, 3-D system supported continuous tissue development, while 2-D system could not sustain long-term development with the loss of cells. The results suggested that the intimate spatial cell-cell and cell-matrix interactions controlled by the 3-D pore structure are essential for normal tissue development and function.
520 $a As a further application of 3-D culture technology, human cord blood cells were cultured in this non-woven matrix, in an attempt to simulate bone marrow microenvironment and expand progenitors. The 3-D cell organizations were formed in a developmental manner as examined by scanning electron microscopy and histological sections. Both CD45<super>+</super> and CD45<super>− </super> adherent cells were spatially distributed within 3-D matrix. The 3-D culture supported more total cells and progenitors compared to 2-D culture, suggesting that 3-D microenvironment promoted progenitor production. The effect of serum was also evaluated because the animal serum may cause immune response in clinical trials. Serum-free medium supported similar or higher progenitor production compared to serum-containing medium. The 3-D system also showed the beneficial effect using serum-free medium, especially in later stage of the culture. Furthermore, serum free culture produced more balanced blood cell population and led to different adherent cells in 3-D matrix, with CD45<super> +</super> and CD41a<super>+</super> cells instead of CD14<super>+</super> cells. Finally, murine embryonic stem cells were cultured for deriving hematopoietic cells. A perfusion bioreactor with 3-D fibrous matrix was studied as a potential large-scale process for stem cell culture.
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650 4 $a Engineering, Chemical. $3 1018531
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