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The development of Sleeping Beauty g...

Clark, Karl Joseph.

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The development of Sleeping Beauty gene-trap transposons for insertional mutagenesis of vertebrates.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	The development of Sleeping Beauty gene-trap transposons for insertional mutagenesis of vertebrates./
Author:	Clark, Karl Joseph.
Description:	117 p.
Notes:	Adviser: Perry B. Hackett.
Contained By:	Dissertation Abstracts International64-04B.
Subject:	Biology, Genetics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3087745

The development of Sleeping Beauty gene-trap transposons for insertional mutagenesis of vertebrates.
Clark, Karl Joseph.

The development of Sleeping Beauty gene-trap transposons for insertional mutagenesis of vertebrates. - 117 p.

Adviser: Perry B. Hackett.

Thesis (Ph.D.)--University of Minnesota, 2003.

To further our understanding of biology and ultimately improve human health through medical advances, it is desirable to learn the function(s) of the genes that comprise the human genome. In order to learn the function of vertebrate genes, methods of high throughput functional analysis in vertebrate model organisms are required. The zebrafish (<italic>Danio rerio </italic>) has the capacity to be used as a high throughput model organism for functional genomic studies. A preferred way to understand a gene's function(s) is to determine the phenotype(s) that become apparent when the gene's product is expressed incorrectly or missing entirely. One way to disrupt a gene is through insertional mutagenesis by the incorporation of DNA into the genome. An efficient way to delivery DNA for insertional mutagenesis is to use a transposon. The <italic>Sleeping Beauty</italic> transposon system can be used in vertebrates, like the zebrafish. Several specialized vectors for insertional mutagenesis, often referred to as “trap” vectors, can be used to mutate genes while quickly learning about the normal expression pattern of the disrupted gene. We have developed the <italic>Sleeping Beauty</italic> system to function as a trap vector for insertional mutagenesis in vertebrates. We constructed and tested several transposon trap vectors in both human cells and zebrafish. Transgenic zebrafish have been produced that can be used in a high-throughput insertional mutagenesis screen to discover the function of thousands of genes in a vertebrate model organism.Subjects--Topical Terms:

1017730
Biology, Genetics.

The development of Sleeping Beauty gene-trap transposons for insertional mutagenesis of vertebrates.
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005 20110509
008 110509s2003 eng d
035 $a (UnM)AAI3087745
035 $a AAI3087745
040 $a UnM $c UnM
100 1 $a Clark, Karl Joseph. $3 1258442
245 1 0 $a The development of Sleeping Beauty gene-trap transposons for insertional mutagenesis of vertebrates.
300 $a 117 p.
500 $a Adviser: Perry B. Hackett.
500 $a Source: Dissertation Abstracts International, Volume: 64-04, Section: B, page: 1598.
502 $a Thesis (Ph.D.)--University of Minnesota, 2003.
520 $a To further our understanding of biology and ultimately improve human health through medical advances, it is desirable to learn the function(s) of the genes that comprise the human genome. In order to learn the function of vertebrate genes, methods of high throughput functional analysis in vertebrate model organisms are required. The zebrafish (<italic>Danio rerio </italic>) has the capacity to be used as a high throughput model organism for functional genomic studies. A preferred way to understand a gene's function(s) is to determine the phenotype(s) that become apparent when the gene's product is expressed incorrectly or missing entirely. One way to disrupt a gene is through insertional mutagenesis by the incorporation of DNA into the genome. An efficient way to delivery DNA for insertional mutagenesis is to use a transposon. The <italic>Sleeping Beauty</italic> transposon system can be used in vertebrates, like the zebrafish. Several specialized vectors for insertional mutagenesis, often referred to as “trap” vectors, can be used to mutate genes while quickly learning about the normal expression pattern of the disrupted gene. We have developed the <italic>Sleeping Beauty</italic> system to function as a trap vector for insertional mutagenesis in vertebrates. We constructed and tested several transposon trap vectors in both human cells and zebrafish. Transgenic zebrafish have been produced that can be used in a high-throughput insertional mutagenesis screen to discover the function of thousands of genes in a vertebrate model organism.
590 $a School code: 0130.
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Molecular. $3 1017719
690 $a 0307
690 $a 0369
710 2 0 $a University of Minnesota. $3 676231
773 0 $t Dissertation Abstracts International $g 64-04B.
790 $a 0130
790 1 0 $a Hackett, Perry B., $e advisor
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3087745