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The development of Sleeping Beauty g...

Clark, Karl Joseph.

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The development of Sleeping Beauty gene-trap transposons for insertional mutagenesis of vertebrates.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	The development of Sleeping Beauty gene-trap transposons for insertional mutagenesis of vertebrates./
作者:	Clark, Karl Joseph.
面頁冊數:	117 p.
附註:	Adviser: Perry B. Hackett.
Contained By:	Dissertation Abstracts International64-04B.
標題:	Biology, Genetics. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3087745

The development of Sleeping Beauty gene-trap transposons for insertional mutagenesis of vertebrates.
Clark, Karl Joseph.

The development of Sleeping Beauty gene-trap transposons for insertional mutagenesis of vertebrates. - 117 p.

Adviser: Perry B. Hackett.

Thesis (Ph.D.)--University of Minnesota, 2003.

To further our understanding of biology and ultimately improve human health through medical advances, it is desirable to learn the function(s) of the genes that comprise the human genome. In order to learn the function of vertebrate genes, methods of high throughput functional analysis in vertebrate model organisms are required. The zebrafish (<italic>Danio rerio </italic>) has the capacity to be used as a high throughput model organism for functional genomic studies. A preferred way to understand a gene's function(s) is to determine the phenotype(s) that become apparent when the gene's product is expressed incorrectly or missing entirely. One way to disrupt a gene is through insertional mutagenesis by the incorporation of DNA into the genome. An efficient way to delivery DNA for insertional mutagenesis is to use a transposon. The <italic>Sleeping Beauty</italic> transposon system can be used in vertebrates, like the zebrafish. Several specialized vectors for insertional mutagenesis, often referred to as “trap” vectors, can be used to mutate genes while quickly learning about the normal expression pattern of the disrupted gene. We have developed the <italic>Sleeping Beauty</italic> system to function as a trap vector for insertional mutagenesis in vertebrates. We constructed and tested several transposon trap vectors in both human cells and zebrafish. Transgenic zebrafish have been produced that can be used in a high-throughput insertional mutagenesis screen to discover the function of thousands of genes in a vertebrate model organism.Subjects--Topical Terms:

1017730
Biology, Genetics.

The development of Sleeping Beauty gene-trap transposons for insertional mutagenesis of vertebrates.
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502 $a Thesis (Ph.D.)--University of Minnesota, 2003.
520 $a To further our understanding of biology and ultimately improve human health through medical advances, it is desirable to learn the function(s) of the genes that comprise the human genome. In order to learn the function of vertebrate genes, methods of high throughput functional analysis in vertebrate model organisms are required. The zebrafish (<italic>Danio rerio </italic>) has the capacity to be used as a high throughput model organism for functional genomic studies. A preferred way to understand a gene's function(s) is to determine the phenotype(s) that become apparent when the gene's product is expressed incorrectly or missing entirely. One way to disrupt a gene is through insertional mutagenesis by the incorporation of DNA into the genome. An efficient way to delivery DNA for insertional mutagenesis is to use a transposon. The <italic>Sleeping Beauty</italic> transposon system can be used in vertebrates, like the zebrafish. Several specialized vectors for insertional mutagenesis, often referred to as “trap” vectors, can be used to mutate genes while quickly learning about the normal expression pattern of the disrupted gene. We have developed the <italic>Sleeping Beauty</italic> system to function as a trap vector for insertional mutagenesis in vertebrates. We constructed and tested several transposon trap vectors in both human cells and zebrafish. Transgenic zebrafish have been produced that can be used in a high-throughput insertional mutagenesis screen to discover the function of thousands of genes in a vertebrate model organism.
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