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Isolation and characterization of a ...

Guo, Wei.

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Isolation and characterization of a novel human Mix-like homeobox gene MIXL.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Isolation and characterization of a novel human Mix-like homeobox gene MIXL./
作者:	Guo, Wei.
面頁冊數:	148 p.
附註:	Source: Dissertation Abstracts International, Volume: 63-11, Section: B, page: 5062.
Contained By:	Dissertation Abstracts International63-11B.
標題:	Biology, Genetics. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3070961
ISBN:	0493906096

Isolation and characterization of a novel human Mix-like homeobox gene MIXL.
Guo, Wei.

Isolation and characterization of a novel human Mix-like homeobox gene MIXL. - 148 p.

Source: Dissertation Abstracts International, Volume: 63-11, Section: B, page: 5062.

Thesis (Ph.D.)--The University of Texas Health Science Center at Houston Graduate School of Biomedical Sciences, 2002.

Taken together, isolation of the <italic>MIXL</italic> gene is the first step toward understanding novel regulatory circuits in early hematopoietic differentiation and malignant transformation.

ISBN: 0493906096Subjects--Topical Terms:

1017730
Biology, Genetics.

Isolation and characterization of a novel human Mix-like homeobox gene MIXL.
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245 1 0 $a Isolation and characterization of a novel human Mix-like homeobox gene MIXL.
300 $a 148 p.
500 $a Source: Dissertation Abstracts International, Volume: 63-11, Section: B, page: 5062.
500 $a Supervisor: Lalitha Nagarajan.
502 $a Thesis (Ph.D.)--The University of Texas Health Science Center at Houston Graduate School of Biomedical Sciences, 2002.
520 $a Taken together, isolation of the <italic>MIXL</italic> gene is the first step toward understanding novel regulatory circuits in early hematopoietic differentiation and malignant transformation.
520 $a Molecular events involved in specification of early hematopoietic system are not well known. In <italic>Xenopus</italic>, a paired-box homeodomain family (Mix.1–4) has been implicated in this process. Although Mix-like homeobox genes have been isolated from zebrafish (<italic>bon</italic>), chicken (<italic>CMIX</italic>) and mice (<italic>MmI/MIXL1</italic>), isolation of a human Mix-like gene has remained elusive.
520 $a We have recently isolated and characterized a novel human Mix-like homeobox gene with a predicted open reading frame of 232 amino acids designated the Mix.1 homeobox (<italic>Xenopus laevis</italic>)-like gene (<italic>MIXL</italic>). The overall identity of this novel protein to CMIX and MmI/MIXL1 is 41% and 69%, respectively. However, the identity in the homeodomain is 66% to that of <italic>Xenopus</italic> Mix.1, 79% to that of CMIX, and 94% to that of MmI/MIXL1. In normal hematopoiesis, <italic>MIXL</italic> expression appears to be restricted immature B and T lymphoid cells. Several acute leukemic cell lines of B, T and myeloid lineages express MIXL suggesting a survival/block in differentiation advantage. Furthermore, <italic>Xenopus</italic> animal cap assay revealed that MIXL could induce expression of the α-globin gene, suggesting a functional conservation of the homeodomain.
520 $a Biochemical analysis revealed that MIXL proteins are phosphorylated at multiple sites. Immunoprecipitation and immunoblotting confirmed that MIXL is tyrosine phosphorylated. Mutational analysis determined that Tyr20 appears to be the site for phosphorylation. However, deletion analysis preliminarily showed that the proline-rich domain appears not to be necessary for tyrosine phosphorylation. The novel finding will help us make a deeper understanding of the regulation on homeodomain proteins by rarely reported tyrosine phosphorylation.
590 $a School code: 2034.
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710 2 0 $a The University of Texas Health Science Center at Houston Graduate School of Biomedical Sciences. $3 1251166
773 0 $t Dissertation Abstracts International $g 63-11B.
790 $a 2034
790 1 0 $a Nagarajan, Lalitha, $e advisor
791 $a Ph.D.
792 $a 2002
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