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Human tear protein analysis, and fac...

Ng, Sheung-Shun Vincent.

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Human tear protein analysis, and factors affecting the concentrations of total and individual tear proteins.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Human tear protein analysis, and factors affecting the concentrations of total and individual tear proteins./
作者:	Ng, Sheung-Shun Vincent.
面頁冊數:	286 p.
附註:	Adviser: Pauline Cho.
Contained By:	Dissertation Abstracts International63-10B.
標題:	Health Sciences, Ophthalmology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3066468
ISBN:	0493859217

Human tear protein analysis, and factors affecting the concentrations of total and individual tear proteins.
Ng, Sheung-Shun Vincent.

Human tear protein analysis, and factors affecting the concentrations of total and individual tear proteins. - 286 p.

Adviser: Pauline Cho.

Thesis (Ph.D.)--Hong Kong Polytechnic (People's Republic of China), 2002.

Although different values of TTPC were obtained with different methods/standards used, their values were found correlated well with each other. Our results showed good agreements between the measured and the calculated TTPC.

ISBN: 0493859217Subjects--Topical Terms:

1019445
Health Sciences, Ophthalmology.

Human tear protein analysis, and factors affecting the concentrations of total and individual tear proteins.
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100 1 $a Ng, Sheung-Shun Vincent. $3 1257694
245 1 0 $a Human tear protein analysis, and factors affecting the concentrations of total and individual tear proteins.
300 $a 286 p.
500 $a Adviser: Pauline Cho.
500 $a Source: Dissertation Abstracts International, Volume: 63-10, Section: B, page: 4608.
502 $a Thesis (Ph.D.)--Hong Kong Polytechnic (People's Republic of China), 2002.
520 $a Although different values of TTPC were obtained with different methods/standards used, their values were found correlated well with each other. Our results showed good agreements between the measured and the calculated TTPC.
520 $a There were no significant daytime and between-day variations in TTPC and the individual tear proteins, except for the concentration of HSA. The eye-flush method consistently showed greater variability than the yawn method.
520 $a The objectives of this study were to analyze human tear protein profiles, and to investigate the factors affecting the total tear protein concentration (TTPC) and the individual proteins. Tear samples were collected (using capillary tube) from young normal Hong Kong-Chinese and TTPC and individual protein concentrations were determined.
520 $a In the main studies, we determined the tear protein levels of young Hong Kong-Chinese. The results of TTPC obtained using different methods (Bradford or Lowry) and standards (bovine serum albumin (BSA) and bovine immunoglobulin G (IgG)) were compared, and their relationships were determined.
520 $a The Bradford and Lowry methods were repeatable in the determination of TTPC. The technique of SDS-PAGE was found to have clinically acceptable repeatability in the determination of lactoferrin, lysozyme and tear specific prealbumin (TSP). However, the variability of immunoglobulin A (sIgA) and human serum albumin (HSA) levels were of clinical significance.
520 $a For the Bradford method using BSA and IgG as standards, the mean TTPC were 6.05 ± 1.58 and 11.48 ± 2.32 mg/ml respectively. For the Lowry method using BSA and IgG as standards, the mean TTPC were 9.66 ± 2.03 and 7.53 ± 1.80 mg/ml respectively. Using SDS-PAGE, protein bands of sIgA, lactoferrin, HSA, TSP and lysozyme were separated. The mean concentrations of sIgA and lactoferrin were 0.29 ± 0.10 and 2.73 ± 0.82 mg/ml respectively. The mean concentrations of HSA, TSP and lysozyme were 0.021 ± 0.028, 2.89 ± 0.88 and 2.46 ± 0.44 mg/ml respectively.
520 $a Using known concentration artificial tear protein mixtures to simulate tears from normal and dry eye subjects, we found a standard comprising a mixture of 50% BSA and 50% IgG would provide a more accurate and better estimation of TTPC than if either BSA or IgG was used. For the Lowry method, using either IgG or IgA as standard would give more accurate results than using BSA, HSA or lysozyme standards.
520 $a We did not observed any significant difference in the tear protein profiles between contact lens (n = 14) and non-contact lens (n = 14) wearers. There were no significant changes in tear protein levels before and after contact lens wear (both soft (n = 7) and rigid (n = 7) lens wear). (Abstract shortened by UMI.)
590 $a School code: 1170.
650 4 $a Health Sciences, Ophthalmology. $3 1019445
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710 2 0 $a Hong Kong Polytechnic (People's Republic of China). $3 1249353
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790 $a 1170
790 1 0 $a Cho, Pauline, $e advisor
791 $a Ph.D.
792 $a 2002
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