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Bacillus thuringiensis strain M15, a...

Jung, Yong-Chul.

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Bacillus thuringiensis strain M15, a novel autoagglutinable, nonserotypeable strain-cloning and characterization of a novel cry31A-type crystal protein gene, cry31Aa2, and two new insertion sequences, IS231M and -N.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Bacillus thuringiensis strain M15, a novel autoagglutinable, nonserotypeable strain-cloning and characterization of a novel cry31A-type crystal protein gene, cry31Aa2, and two new insertion sequences, IS231M and -N./
Author:	Jung, Yong-Chul.
Description:	145 p.
Notes:	Advisers: Young-Sup Chung; Jean-Charles Cote.
Contained By:	Dissertation Abstracts International63-01B.
Subject:	Biology, Entomology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NQ65709
ISBN:	0612657094

Bacillus thuringiensis strain M15, a novel autoagglutinable, nonserotypeable strain-cloning and characterization of a novel cry31A-type crystal protein gene, cry31Aa2, and two new insertion sequences, IS231M and -N.
Jung, Yong-Chul.

Bacillus thuringiensis strain M15, a novel autoagglutinable, nonserotypeable strain-cloning and characterization of a novel cry31A-type crystal protein gene, cry31Aa2, and two new insertion sequences, IS231M and -N. - 145 p.

Advisers: Young-Sup Chung; Jean-Charles Cote.

Thesis (Ph.D.)--Universite de Montreal (Canada), 2002.

The synthetic organic pesticides have caused considerable damage on the environment and human health due to their accumulation in the ecosystem and the contamination of the food chain in the environment. Several resistances to synthetic insecticides have appeared in some insect pest populations. For these reasons, there is a need for the development of biological control agents as an alternative for the management of insect pests.

ISBN: 0612657094Subjects--Topical Terms:

1018619
Biology, Entomology.

Bacillus thuringiensis strain M15, a novel autoagglutinable, nonserotypeable strain-cloning and characterization of a novel cry31A-type crystal protein gene, cry31Aa2, and two new insertion sequences, IS231M and -N.
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020 $a 0612657094
035 $a (UnM)AAINQ65709
035 $a AAINQ65709
040 $a UnM $c UnM
100 1 $a Jung, Yong-Chul. $3 1257006
245 1 0 $a Bacillus thuringiensis strain M15, a novel autoagglutinable, nonserotypeable strain-cloning and characterization of a novel cry31A-type crystal protein gene, cry31Aa2, and two new insertion sequences, IS231M and -N.
300 $a 145 p.
500 $a Advisers: Young-Sup Chung; Jean-Charles Cote.
500 $a Source: Dissertation Abstracts International, Volume: 63-01, Section: B, page: 0089.
502 $a Thesis (Ph.D.)--Universite de Montreal (Canada), 2002.
520 $a The synthetic organic pesticides have caused considerable damage on the environment and human health due to their accumulation in the ecosystem and the contamination of the food chain in the environment. Several resistances to synthetic insecticides have appeared in some insect pest populations. For these reasons, there is a need for the development of biological control agents as an alternative for the management of insect pests.
520 $a <italic>Bacillus thuringiensis</italic> is the leading biocontrol agent and has been used as a biorational pesticide in insect pest control. <italic> B. thuringiensis</italic> is a Gram-positive, spore-forming bacterium that is commonly found in natural environments. During the stationary phase of its growth cycle, <italic>B. thuringiensis</italic> produces a parasporal inclusion that exhibits specific insecticidal activity against certain insect species among Lepidoptera, Diptera, and Coleoptera. The parasporal inclusions are made of proteins. These are encoded by toxin genes. Our research project was to isolate and characterize a new <italic>B. thuringiensis</italic> strain from the non-insect, two-spotted spider mite (<italic>Tetranychus urticae </italic> Koch; Arthropoda: Arachnida: Tetranychidae). A novel crystal protein gene was cloned and sequenced. It was designated <italic>cry31Aa2</italic> by the <italic>B. thuringiensis</italic> Pesticide Crystal Protein Nomenclature Committee. It was expressed in an acrystalliferous <italic>B. thuringiensis </italic> strain. The parasporal inclusion protein expressed was isolated and confirmed to be composed of single major polypeptide of 83-kDa (Chapter 1).
520 $a Insertion sequences and transposons have been found in the vicinity of <italic> cry</italic> genes. We have cloned and characterized two new insertion sequences of the IS<italic>231</italic> family, IS<italic>231</italic>M (Chapter 2) and N (Chapter 3). Although analyses of their DNA sequences revealed high homology to the IS<italic>231</italic> family, their structural maps were different. IS<italic>231</italic>M has two overlapping open reading frames, ORF1 and ORF2, that could encode polypeptides of 334 and 143 amino acids, respectively, while IS<italic>231</italic>N contains three open reading frames (ORFs) that could code for polypeptides of 329 (ORF1), 118 (ORF2) and 17 (ORF3) amino acids, respectively. IS<italic>231</italic>N shows 99% nucleotide identity to IS<italic>231</italic>M, but shares only 83% amino acid identity because of nucleotide substitutions and additions. Whether IS<italic>231</italic>M and -N are functional transposable elements remains to be determined.
590 $a School code: 0992.
650 4 $a Biology, Entomology. $3 1018619
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Engineering, Agricultural. $3 1019504
650 4 $a Environmental Sciences. $3 676987
690 $a 0307
690 $a 0353
690 $a 0410
690 $a 0539
690 $a 0768
710 2 0 $a Universite de Montreal (Canada). $3 1018132
773 0 $t Dissertation Abstracts International $g 63-01B.
790 $a 0992
790 1 0 $a Chung, Young-Sup, $e advisor
790 1 0 $a Cote, Jean-Charles, $e advisor
791 $a Ph.D.
792 $a 2002
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NQ65709