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The molecular microbial ecology and ...

Bost, Fredrick Daniel.

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The molecular microbial ecology and mechanisms of biomarker degradation in crude oil.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	The molecular microbial ecology and mechanisms of biomarker degradation in crude oil./
Author:	Bost, Fredrick Daniel.
Description:	227 p.
Notes:	Chairperson: Pamela J. Morris.
Contained By:	Dissertation Abstracts International62-06B.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3017722
ISBN:	0493280081

The molecular microbial ecology and mechanisms of biomarker degradation in crude oil.
Bost, Fredrick Daniel.

The molecular microbial ecology and mechanisms of biomarker degradation in crude oil. - 227 p.

Chairperson: Pamela J. Morris.

Thesis (Ph.D.)--Medical University of South Carolina, 2001.

The studies presented here used bacterial enrichment cultures to examine the distribution and extent of biomarker-degrading activity in aerobic and anaerobic environments.

ISBN: 0493280081Subjects--Topical Terms:

1017734
Biology, Microbiology.

The molecular microbial ecology and mechanisms of biomarker degradation in crude oil.
LDR:03748nam 2200301 a 45 001 932563
005 20110505
008 110505s2001 eng d
020 $a 0493280081
035 $a (UnM)AAI3017722
035 $a AAI3017722
040 $a UnM $c UnM
100 1 $a Bost, Fredrick Daniel. $3 1256303
245 1 0 $a The molecular microbial ecology and mechanisms of biomarker degradation in crude oil.
300 $a 227 p.
500 $a Chairperson: Pamela J. Morris.
500 $a Source: Dissertation Abstracts International, Volume: 62-06, Section: B, page: 2592.
502 $a Thesis (Ph.D.)--Medical University of South Carolina, 2001.
520 $a The studies presented here used bacterial enrichment cultures to examine the distribution and extent of biomarker-degrading activity in aerobic and anaerobic environments.
520 $a The biodegradation of C30 17α,21β(H)-hopane within Bonny Light crude oil by laboratory enrichment cultures was demonstrated to be temperature sensitive through incubation at 4°C, 15°C, 30°C, and 37°C. Although degradation of the <italic>n</italic>-alkanes and the branched alkanes, pristane and phytane, was observed at 15°C, 30°C, and 37°C, the degradation of hopane was limited to 30°C. At 4°C, no degradation of any crude oil components was observed. DGGE analysis of the cultures at these different temperatures revealed no striking differences in the composition of the microbial communities.
520 $a The aerobic microbial degradation of two Venezuelan crude oils enriched in 25-norhopanes was examined after a five-week aerobic incubation using the Light Crude (LC) enrichment culture, an enrichment culture developed from a creosote-contaminated soil and previously shown to degrade the C30 17α,21β(H)-hopane. After five weeks, analysis of the Venezuelan oils using gas chromatography-mass spectrometry revealed extensive biomarker degradation. The C28 tricyclic terpane, C29–C 34 17α(H),21β(H)-hopanes, and the C29 17α(H),21β(H)-25-norhopane were all degraded. The C35 17α(H),21β(H)-hopane and 18α(H)-oleanane were conserved. Further and previously unreported, the C28–C34 17α(H),21β(H)-25-norhopanes were degraded and no formation of 25-norhopanes was observed. Degradation caused preferential removal of the 22<italic>R</italic> versus the 22<italic> S</italic> isomer in both the extended hopanes and 25-norhopanes, implying that bacteria remove these compounds in aerobic environments. These data also demonstrated 25-norhopane degradation on a time scale similar to other biomarkers, suggesting that the 25-norhopanes and hopanes share a microbial mechanism of degradation.
520 $a The potential cometabolic mechanisms of C30 17α,21β-hopane transformation were examined using the Degraded Light Crude (DLC) enrichment culture and a Hopane-Enriched Fraction (HEF) of Bonny Light crude oil. These studies demonstrated hopane degradation within HEF, suggesting that the degradation of C30 17α,21β(H)-hopane by the DLC culture is not cometabolic. The elucidation and description of aerobic and anaerobic mechanisms of biomarker transformation may reveal metabolically distinct routes that can be used as signatures of aerobic and anaerobic transformations. (Abstract shortened by UMI.)
590 $a School code: 0122.
650 4 $a Biology, Microbiology. $3 1017734
690 $a 0410
710 2 0 $a Medical University of South Carolina. $3 700119
773 0 $t Dissertation Abstracts International $g 62-06B.
790 $a 0122
790 1 0 $a Morris, Pamela J., $e advisor
791 $a Ph.D.
792 $a 2001
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3017722