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Analysis of bacterial transport and ...

Kovacik, William Peter, Jr.

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Analysis of bacterial transport and survival in the subsurface.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Analysis of bacterial transport and survival in the subsurface./
Author:	Kovacik, William Peter, Jr.
Description:	132 p.
Notes:	Adviser: William E. Holben.
Contained By:	Dissertation Abstracts International63-08B.
Subject:	Biogeochemistry. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3062630
ISBN:	0493785159

Analysis of bacterial transport and survival in the subsurface.
Kovacik, William Peter, Jr.

Analysis of bacterial transport and survival in the subsurface. - 132 p.

Adviser: William E. Holben.

Thesis (Ph.D.)--University of Montana, 2002.

Many subsurface environments across the United States are contaminated from past xenobiotic discharges, much of it too deep and extensive for conventional methods of remediation. Bacteria might be the best option available for the <italic> bioremediation</italic> of deep subsurface contamination because of their potential to travel to distant contaminated locations, and their ability through metabolic activities to potentially detoxify or limit the further migration of contaminants. However, relatively little is known about the transport capabilities of injected microorganisms into the subsurface, nor their ability to colonize, survive and grow once they reach locations of distant contamination. A basic understanding of both processes is therefore necessary to design strategies for the use of bacteria in remediation of subsurface environments.

ISBN: 0493785159Subjects--Topical Terms:

545717
Biogeochemistry.

Analysis of bacterial transport and survival in the subsurface.
LDR:03339nam 2200313 a 45 001 929445
005 20110427
008 110427s2002 eng d
020 $a 0493785159
035 $a (UnM)AAI3062630
035 $a AAI3062630
040 $a UnM $c UnM
100 1 $a Kovacik, William Peter, Jr. $3 1252932
245 1 0 $a Analysis of bacterial transport and survival in the subsurface.
300 $a 132 p.
500 $a Adviser: William E. Holben.
500 $a Source: Dissertation Abstracts International, Volume: 63-08, Section: B, page: 3574.
502 $a Thesis (Ph.D.)--University of Montana, 2002.
520 $a Many subsurface environments across the United States are contaminated from past xenobiotic discharges, much of it too deep and extensive for conventional methods of remediation. Bacteria might be the best option available for the <italic> bioremediation</italic> of deep subsurface contamination because of their potential to travel to distant contaminated locations, and their ability through metabolic activities to potentially detoxify or limit the further migration of contaminants. However, relatively little is known about the transport capabilities of injected microorganisms into the subsurface, nor their ability to colonize, survive and grow once they reach locations of distant contamination. A basic understanding of both processes is therefore necessary to design strategies for the use of bacteria in remediation of subsurface environments.
520 $a My dissertation research project consisted of two studies related to the ecology of bacteria in subsurface environments. The first study involved analysis of the microbial community diversity of shale and sandstone rocks located 200 meters below Cerro Negro, New Mexico. The objective was to increase our understanding of the microbial ecology of deep subsurface environments typical of many contaminated sites. The second involved studies of short-term temporal transport of bacteria, in laboratory column experiments and <italic> in situ</italic> injection experiments at a field site in Oyster, Virginia. The objectives were to develop methods of accurately monitoring bacterial transport and to determine the factors that control transport of bacteria in subsurface environments.
520 $a In the study of the ecology of the deep subsurface shale/sandstone interface at Cerro Negro, we found that the geochemistry of a site alone is not adequate to predict the types of organisms present. We found a predominance of organisms capable of Fe(III) reduction in an environment where sulfate reducing microbes were expected to dominate based on the geochemistry of the site. Therefore the design of remediation strategies must account for the Fe(III) reducing bacteria. In the Oyster transport study, we were able to demonstrate the <italic> in situ</italic> transport of adherence-deficient microbes, and their subsequent attachment and growth in aquifer sediments, demonstrating that bioremediation using injected microorganisms was feasible for subsurface contamination.
590 $a School code: 0136.
650 4 $a Biogeochemistry. $3 545717
650 4 $a Biology, Ecology. $3 1017726
650 4 $a Biology, Microbiology. $3 1017734
690 $a 0329
690 $a 0410
690 $a 0425
710 2 0 $a University of Montana. $3 1021963
773 0 $t Dissertation Abstracts International $g 63-08B.
790 $a 0136
790 1 0 $a Holben, William E., $e advisor
791 $a Ph.D.
792 $a 2002
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3062630