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The interactions of transcription re...

Nickles, Bryce Edward.

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The interactions of transcription regulators with the sigma(70) subunit of RNA polymerase.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	The interactions of transcription regulators with the sigma(70) subunit of RNA polymerase./
Author:	Nickles, Bryce Edward.
Description:	183 p.
Notes:	Adviser: Ann Hochschild.
Contained By:	Dissertation Abstracts International63-10B.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3067424
ISBN:	0493868623

The interactions of transcription regulators with the sigma(70) subunit of RNA polymerase.
Nickles, Bryce Edward.

The interactions of transcription regulators with the sigma(70) subunit of RNA polymerase. - 183 p.

Adviser: Ann Hochschild.

Thesis (Ph.D.)--Harvard University, 2002.

In bacteria, all genes are transcribed by a single multisubunit entity called RNA polymerase (RNAP). The enzymatic core of RNAP contains all of the requisite machinery for RNA synthesis. Nevertheless, in order to recognize specific promoters and initiate transcription, the core enzyme must associate with a σ factor, which makes direct contact with conserved promoter elements. The primary σ factor in <italic>E. coli</italic>, σ<super> 70</super>, is responsible for the expression of the majority of genes in exponentially growing cells. As well as playing a critical role; in promoter recognition, σ<super>70</super> is well-characterized as a target for proteins that regulate transcription initiation.

ISBN: 0493868623Subjects--Topical Terms:

1017734
Biology, Microbiology.

The interactions of transcription regulators with the sigma(70) subunit of RNA polymerase.
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008 110425s2002 eng d
020 $a 0493868623
035 $a (UnM)AAI3067424
035 $a AAI3067424
040 $a UnM $c UnM
100 1 $a Nickles, Bryce Edward. $3 1251374
245 1 0 $a The interactions of transcription regulators with the sigma(70) subunit of RNA polymerase.
300 $a 183 p.
500 $a Adviser: Ann Hochschild.
500 $a Source: Dissertation Abstracts International, Volume: 63-10, Section: B, page: 4487.
502 $a Thesis (Ph.D.)--Harvard University, 2002.
520 $a In bacteria, all genes are transcribed by a single multisubunit entity called RNA polymerase (RNAP). The enzymatic core of RNAP contains all of the requisite machinery for RNA synthesis. Nevertheless, in order to recognize specific promoters and initiate transcription, the core enzyme must associate with a σ factor, which makes direct contact with conserved promoter elements. The primary σ factor in <italic>E. coli</italic>, σ<super> 70</super>, is responsible for the expression of the majority of genes in exponentially growing cells. As well as playing a critical role; in promoter recognition, σ<super>70</super> is well-characterized as a target for proteins that regulate transcription initiation.
520 $a Until recently, it has been thought that σ<super>70</super> is released from the core enzyme during the transition from the initiation to the elongation phase of transcription. However, emerging evidence suggests that promoter escape may not necessarily require the complete release of σ<super> 70</super> from core. In fact, models based on recently obtained structural data posit a staged (rather than complete) displacement of σ<super> 70</super> from the core enzyme during the transition from initiation to elongation.
520 $a This thesis illustrates how interactions between regulators of transcription and the σ<super>70</super> subunit of RNAP can be exploited during different stages of the transcription process. In particular, I have characterized the interactions of two transcription regulators with the C-terminal domain (conserved region 4) of σ<super>70</super>. One of these regulators, the bacteriophage λ cI protein, is an activator of transcription initiation. In Chapter 21 demonstrate the use of a genetic assay and structural modeling to implicate two amino acid residues within region 4 of σ<super>70</super> in the process of λcI-mediated activation. The other regulator that I have studied, the bacteriophage λ Q antiterminator protein, acts during elongation. In Chapter 3 I demonstrate that λQ contacts region 4 of σ<super>70</super> when λQ engages a paused elongation complex. I show further that this engagement results in a conformation of the RNAP holoenzyme that requires the partial release of σ<super>70</super> from core. These results demonstrate that σ<super> 70</super> can be a target for a regulator that exerts its effect on transcription at a stage after the initiation process and provide evidence for the staged displacement of σ<super>70</super> during the transition from transcription initiation to transcription elongation.
590 $a School code: 0084.
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biology, Molecular. $3 1017719
690 $a 0307
690 $a 0410
710 2 0 $a Harvard University. $3 528741
773 0 $t Dissertation Abstracts International $g 63-10B.
790 $a 0084
790 1 0 $a Hochschild, Ann, $e advisor
791 $a Ph.D.
792 $a 2002
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3067424