東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Meiotic chromosome pairing in Caenor...

MacQueen, Amy Joy.

Linked to FindBook

Google Book

Amazon

博客來

Meiotic chromosome pairing in Caenorhabditis elegans.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Meiotic chromosome pairing in Caenorhabditis elegans./
Author:	MacQueen, Amy Joy.
Description:	174 p.
Notes:	Adviser: Anne M. Villeneuve.
Contained By:	Dissertation Abstracts International63-04B.
Subject:	Biology, Cell. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3048577
ISBN:	0493629084

Meiotic chromosome pairing in Caenorhabditis elegans.
MacQueen, Amy Joy.

Meiotic chromosome pairing in Caenorhabditis elegans. - 174 p.

Adviser: Anne M. Villeneuve.

Thesis (Ph.D.)--Stanford University, 2002.

Cytological analysis of chromosome organization throughout meiotic prophase in <italic>C. elegans</italic> meiotic chromosome segregation mutants has enabled us to identify genes that function in the process of homologous chromosome pairing. Analysis of mutant worms lacking the function of these pairing genes have revealed three distinct mechanisms that contribute to the productive alignment of homologous chromosomes during meiosis. This work has uncovered a role for a <italic>C. elegans chk</italic>-2 protein kinase in the early establishment of initial paired associations between chromosomes. The additional discovery of a similar role for a meiosis-specific chromosome axis protein, HIM-3, raises the possibility that modulation of the chromosome axis following premeiotic S phase could be a key event in the formation of “pairing-competent” chromosomes. In addition, CHK-2, HIM-3, and the product of an additional gene, <italic> hal-2</italic>, represent the first molecular links between initial homolog pairing and the early prophase nuclear reorganization that normally accompanies initial pairing, strengthening the notion that these two major meiotic events are mechanistically coupled. In contrast, <italic>syp-1</italic> and <italic> syp-2</italic> define a class of genes that are dispensable for early prophase nuclear reorganization and the initial establishment of pairing, but are required for maintaining homolog alignment during later prophase stages. Analysis of SYP-1 and SYP-2 suggests that these proteins are structural components of the <italic>C. elegans</italic> synaptonemal complex (SC). Analysis of chromosome pairing in <italic>syp-1</italic> and <italic>syp-2</italic> mutants not only indicates a role for the SC in stabilizing homolog alignment during prophase, but additionally implicates the assembly or function of the SC in driving chromosome redispersal out of the highly polarized distribution that chromosomes adopt at the onset of meiotic prophase. In addition, a severe reduction in crossover formation together with evidence for accumulated recombination intermediates in <italic>syp-1</italic> mutant meiocytes suggests a role for the SC in promoting crossover recombination. Finally, timecourse analysis of homolog pairing in <italic> syp-1</italic> mutants revealed that <italic>cis</italic>-acting chromosome regions can locally stabilize homologous pairing even in the absence of synapsis. I suggest that this stabilization activity might play a key role in enforcing homolog recognition subsequent to or independent of initial pairing, and prior to synapsis.

ISBN: 0493629084Subjects--Topical Terms:

1017686
Biology, Cell.

Meiotic chromosome pairing in Caenorhabditis elegans.
LDR:03482nam 2200277 a 45 001 927716
005 20110425
008 110425s2002 eng d
020 $a 0493629084
035 $a (UnM)AAI3048577
035 $a AAI3048577
040 $a UnM $c UnM
100 1 $a MacQueen, Amy Joy. $3 1251279
245 1 0 $a Meiotic chromosome pairing in Caenorhabditis elegans.
300 $a 174 p.
500 $a Adviser: Anne M. Villeneuve.
500 $a Source: Dissertation Abstracts International, Volume: 63-04, Section: B, page: 1649.
502 $a Thesis (Ph.D.)--Stanford University, 2002.
520 $a Cytological analysis of chromosome organization throughout meiotic prophase in <italic>C. elegans</italic> meiotic chromosome segregation mutants has enabled us to identify genes that function in the process of homologous chromosome pairing. Analysis of mutant worms lacking the function of these pairing genes have revealed three distinct mechanisms that contribute to the productive alignment of homologous chromosomes during meiosis. This work has uncovered a role for a <italic>C. elegans chk</italic>-2 protein kinase in the early establishment of initial paired associations between chromosomes. The additional discovery of a similar role for a meiosis-specific chromosome axis protein, HIM-3, raises the possibility that modulation of the chromosome axis following premeiotic S phase could be a key event in the formation of “pairing-competent” chromosomes. In addition, CHK-2, HIM-3, and the product of an additional gene, <italic> hal-2</italic>, represent the first molecular links between initial homolog pairing and the early prophase nuclear reorganization that normally accompanies initial pairing, strengthening the notion that these two major meiotic events are mechanistically coupled. In contrast, <italic>syp-1</italic> and <italic> syp-2</italic> define a class of genes that are dispensable for early prophase nuclear reorganization and the initial establishment of pairing, but are required for maintaining homolog alignment during later prophase stages. Analysis of SYP-1 and SYP-2 suggests that these proteins are structural components of the <italic>C. elegans</italic> synaptonemal complex (SC). Analysis of chromosome pairing in <italic>syp-1</italic> and <italic>syp-2</italic> mutants not only indicates a role for the SC in stabilizing homolog alignment during prophase, but additionally implicates the assembly or function of the SC in driving chromosome redispersal out of the highly polarized distribution that chromosomes adopt at the onset of meiotic prophase. In addition, a severe reduction in crossover formation together with evidence for accumulated recombination intermediates in <italic>syp-1</italic> mutant meiocytes suggests a role for the SC in promoting crossover recombination. Finally, timecourse analysis of homolog pairing in <italic> syp-1</italic> mutants revealed that <italic>cis</italic>-acting chromosome regions can locally stabilize homologous pairing even in the absence of synapsis. I suggest that this stabilization activity might play a key role in enforcing homolog recognition subsequent to or independent of initial pairing, and prior to synapsis.
590 $a School code: 0212.
650 4 $a Biology, Cell. $3 1017686
650 4 $a Biology, Genetics. $3 1017730
690 $a 0369
690 $a 0379
710 2 0 $a Stanford University. $3 754827
773 0 $t Dissertation Abstracts International $g 63-04B.
790 $a 0212
790 1 0 $a Villeneuve, Anne M., $e advisor
791 $a Ph.D.
792 $a 2002
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3048577