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The tricornered and furry genes of D...

University of Virginia.

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The tricornered and furry genes of Drosophila are important for maintaining the integrity of cellular extensions during morphogenesis.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	The tricornered and furry genes of Drosophila are important for maintaining the integrity of cellular extensions during morphogenesis./
Author:	Geng, Wei.
Description:	104 p.
Notes:	Adviser: Paul N. Adler.
Contained By:	Dissertation Abstracts International63-01B.
Subject:	Biology, Cell. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3038987
ISBN:	0493522034

The tricornered and furry genes of Drosophila are important for maintaining the integrity of cellular extensions during morphogenesis.
Geng, Wei.

The tricornered and furry genes of Drosophila are important for maintaining the integrity of cellular extensions during morphogenesis. - 104 p.

Adviser: Paul N. Adler.

Thesis (Ph.D.)--University of Virginia, 2002.

The wings of adult <italic>Drosophila melanogaster</italic> are covered by a large number of small distally pointing hairs, which are formed in the pupal stage from microvilli like prehairs by individual epidermal cells. This dissertation describes my work on the <italic>tricornered</italic> (<italic>trc</italic>) and <italic>furry</italic> (<italic>fry</italic>) genes of <italic>Drosophila</italic>. Mutations in both <italic>trc</italic> and <italic> fry</italic> cause an extreme multiple wing hair phenotype that appears to result from the dramatic splitting of a single prehair early in its elongation. Thus these genes seem to be essential for maintaining prehair integrity.

ISBN: 0493522034Subjects--Topical Terms:

1017686
Biology, Cell.

The tricornered and furry genes of Drosophila are important for maintaining the integrity of cellular extensions during morphogenesis.
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035 $a (UnM)AAI3038987
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100 1 $a Geng, Wei. $3 1250770
245 1 0 $a The tricornered and furry genes of Drosophila are important for maintaining the integrity of cellular extensions during morphogenesis.
300 $a 104 p.
500 $a Adviser: Paul N. Adler.
500 $a Source: Dissertation Abstracts International, Volume: 63-01, Section: B, page: 0086.
502 $a Thesis (Ph.D.)--University of Virginia, 2002.
520 $a The wings of adult <italic>Drosophila melanogaster</italic> are covered by a large number of small distally pointing hairs, which are formed in the pupal stage from microvilli like prehairs by individual epidermal cells. This dissertation describes my work on the <italic>tricornered</italic> (<italic>trc</italic>) and <italic>furry</italic> (<italic>fry</italic>) genes of <italic>Drosophila</italic>. Mutations in both <italic>trc</italic> and <italic> fry</italic> cause an extreme multiple wing hair phenotype that appears to result from the dramatic splitting of a single prehair early in its elongation. Thus these genes seem to be essential for maintaining prehair integrity.
520 $a We have molecularly cloned <italic>trc</italic> using a P insertion that marks the gene. <italic>trc</italic> encodes the <italic>Drosophila</italic> Ndr kinase, a well conserved serine/threonine protein kinase also found in <italic> Caenorhabditis elegans</italic> and humans. Trc is related to a number of kinases that have been shown to regulate cellular morphology. Further evidence that <italic>trc</italic> encodes the fly Ndr protein kinase was obtained by sequencing EMS induced <italic>trc</italic> point mutations and by transformation rescue with <italic>trc</italic> cDNA. Transgenic flies carrying FLAG/(His)<sub> 6</sub> tagged <italic>trc</italic> cDNA were made, and the immunostaining of their tissues showed that the Trc fusion protein is primarily nuclear.
520 $a <italic>fry</italic> was recovered in a FLP/FRT based mutant screen for isolating wing hair polarity mutants. We used a P element insertion allele as a tag to clone the <italic>fry</italic> gene. About 20 kb genomic DNA flanking the P insertion site was cloned and sequenced. Four cDNA candidates from this region were recovered and three of them were subsequently ruled out either by complementation test or by sequencing EMS induced <italic>fry</italic> alleles. A clone of the fourth cDNA candidate was identified and later the full-length cDNA sequence was recovered by fellow graduate student Jingli Cong. She confirmed that this cDNA indeed encodes the <italic>fry</italic> locus by sequencing point mutations. <italic>fry</italic> was found to be a large and complicated gene that encodes a pair of large conserved proteins of unknown biochemical function. Data from double mutant analysis have argued that <italic>fry</italic> and <italic>trc</italic> are likely to function in the same genetic pathway that is independent of the <italic>frizzled</italic> tissue polarity pathway.
590 $a School code: 0246.
650 4 $a Biology, Cell. $3 1017686
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Molecular. $3 1017719
690 $a 0307
690 $a 0369
690 $a 0379
710 2 0 $a University of Virginia. $3 645578
773 0 $t Dissertation Abstracts International $g 63-01B.
790 $a 0246
790 1 0 $a Adler, Paul N., $e advisor
791 $a Ph.D.
792 $a 2002
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3038987