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Genetic and biochemical studies of D...

University of Virginia.

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Genetic and biochemical studies of Drosophila planar polarity.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Genetic and biochemical studies of Drosophila planar polarity./
Author:	Lee, Haeryun.
Description:	171 p.
Notes:	Adviser: Paul N. Adler.
Contained By:	Dissertation Abstracts International62-09B.
Subject:	Biology, Genetics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3027460
ISBN:	0493394133

Genetic and biochemical studies of Drosophila planar polarity.
Lee, Haeryun.

Genetic and biochemical studies of Drosophila planar polarity. - 171 p.

Adviser: Paul N. Adler.

Thesis (Ph.D.)--University of Virginia, 2002.

This thesis reports genetic and biochemical studies directed at the mechanisms underlying planar polarity formation in <italic>Drosophila </italic>. Planar polarity formation requires the <italic>frizzled</italic> pathway, which is a major planar polarity pathway in <italic>Drosophila</italic>. In Chapter 2, I studied the genetic interactions between components of the <italic> frizzled</italic> pathway in the wing. I investigated whether <italic>inturned, mwh</italic>, and <italic>dsh</italic> are required for <italic>frizzled</italic> cell nonautonomous or cell autonomous function. The activity of <italic>inturned, mwh</italic>, and <italic>dsh</italic> was not needed for cells to generate or send the <italic>frizzled</italic>-dependent polarity signal, but was required for cells to receive or respond to this polarity signal, suggesting that <italic> inturned, mwh</italic>, and <italic>dsh</italic> play a role in the <italic> frizzled</italic> cell autonomous function. I determined the genetic hierarchy and relationship between components in the <italic>frizzled</italic> cell autonomous pathway. Gain-of-function phenotypes from directed expression of <italic> frizzled</italic>-like genes were blocked by mutations in <italic>inturned </italic>-like genes, and overexpression of <italic>frizzled</italic>-like genes enhanced the phenotypes of weak <italic>inturned</italic>-like genes. Interestingly, decreased levels of <italic>frizzled</italic>-like genes also enhanced weak <italic>inturned</italic>-like alleles, suggesting that <italic> frizzled</italic>-like genes and <italic>inturned</italic>-like genes are not in a simple negative or positive relationship. These results argue that the activity of <italic>inturned</italic>-like genes is dependent on the status of <italic>frizzled</italic>-like genes in the <italic>frizzled</italic> cell autonomous pathway.

ISBN: 0493394133Subjects--Topical Terms:

1017730
Biology, Genetics.

Genetic and biochemical studies of Drosophila planar polarity.
LDR:04394nam 2200289 a 45 001 927200
005 20110425
008 110425s2002 eng d
020 $a 0493394133
035 $a (UnM)AAI3027460
035 $a AAI3027460
040 $a UnM $c UnM
100 1 $a Lee, Haeryun. $3 1250751
245 1 0 $a Genetic and biochemical studies of Drosophila planar polarity.
300 $a 171 p.
500 $a Adviser: Paul N. Adler.
500 $a Source: Dissertation Abstracts International, Volume: 62-09, Section: B, page: 3894.
502 $a Thesis (Ph.D.)--University of Virginia, 2002.
520 $a This thesis reports genetic and biochemical studies directed at the mechanisms underlying planar polarity formation in <italic>Drosophila </italic>. Planar polarity formation requires the <italic>frizzled</italic> pathway, which is a major planar polarity pathway in <italic>Drosophila</italic>. In Chapter 2, I studied the genetic interactions between components of the <italic> frizzled</italic> pathway in the wing. I investigated whether <italic>inturned, mwh</italic>, and <italic>dsh</italic> are required for <italic>frizzled</italic> cell nonautonomous or cell autonomous function. The activity of <italic>inturned, mwh</italic>, and <italic>dsh</italic> was not needed for cells to generate or send the <italic>frizzled</italic>-dependent polarity signal, but was required for cells to receive or respond to this polarity signal, suggesting that <italic> inturned, mwh</italic>, and <italic>dsh</italic> play a role in the <italic> frizzled</italic> cell autonomous function. I determined the genetic hierarchy and relationship between components in the <italic>frizzled</italic> cell autonomous pathway. Gain-of-function phenotypes from directed expression of <italic> frizzled</italic>-like genes were blocked by mutations in <italic>inturned </italic>-like genes, and overexpression of <italic>frizzled</italic>-like genes enhanced the phenotypes of weak <italic>inturned</italic>-like genes. Interestingly, decreased levels of <italic>frizzled</italic>-like genes also enhanced weak <italic>inturned</italic>-like alleles, suggesting that <italic> frizzled</italic>-like genes and <italic>inturned</italic>-like genes are not in a simple negative or positive relationship. These results argue that the activity of <italic>inturned</italic>-like genes is dependent on the status of <italic>frizzled</italic>-like genes in the <italic>frizzled</italic> cell autonomous pathway.
520 $a In Chapter 3, I studied the biochemical interaction of two <italic>frizzled </italic> downstream components, <italic>inturned</italic> and <italic>fuzzy </italic>. Interned and Fuzzy were co-immunoprecipitated in both cultured cells and transgenic flies with epitope-tagged <italic>inturned</italic> and <italic> fuzzy</italic> cDNAs, suggesting that they physically interact with each other. Co-overexpression of <italic>inturned</italic> and <italic>fuzzy</italic> produced gain-of-function phenotypes similar to loss-of-function phenotypes. This implies that Inturned and Fuzzy act in a functional complex, supporting the biological relevance of the physical interaction.
520 $a In Chapter 4, I studied a newly identified planar polarity gene that encodes a previously known transcription factor, <italic>grainyhead</italic> (<italic>grh</italic>). Phenotypic analyses showed that <italic>grh</italic> has a role in planar polarity in the wing and eye. I tested genetic interactions of <italic>grh</italic> with components in the <italic>frizzled</italic> pathway in the wing. <italic>grh</italic> was epistatic to <italic>frizzled, inturned </italic>, and <italic>mwh</italic>, and resulted in an enhancement of polarity phenotypes in the presence of <italic>frizzled</italic> and <italic>Vang</italic>. These results suggest that <italic>grh</italic> may be part of the <italic> frizzled</italic> pathway. Genetic and biochemical studies of the <italic> frizzled</italic> pathway and identification of a transcription factor will provide an insight into how components interplay to establish polarized organization of cells and tissues.
590 $a School code: 0246.
650 4 $a Biology, Genetics. $3 1017730
690 $a 0369
710 2 0 $a University of Virginia. $3 645578
773 0 $t Dissertation Abstracts International $g 62-09B.
790 $a 0246
790 1 0 $a Adler, Paul N., $e advisor
791 $a Ph.D.
792 $a 2002
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3027460