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Integration of microchip-capillary e...

Liu, Jun.

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Integration of microchip-capillary electrophoresis separation with matrix-assisted laser desorption/ionization (MALDI) Fourier transform mass spectrometry (FTMS) and quantitative determination of oligosaccharide expression levels in individual cells.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Integration of microchip-capillary electrophoresis separation with matrix-assisted laser desorption/ionization (MALDI) Fourier transform mass spectrometry (FTMS) and quantitative determination of oligosaccharide expression levels in individual cells./
作者:	Liu, Jun.
面頁冊數:	125 p.
附註:	Adviser: Calito B. Lebrilla.
Contained By:	Dissertation Abstracts International63-12B.
標題:	Chemistry, Analytical. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3074586
ISBN:	0493950338

Integration of microchip-capillary electrophoresis separation with matrix-assisted laser desorption/ionization (MALDI) Fourier transform mass spectrometry (FTMS) and quantitative determination of oligosaccharide expression levels in individual cells.
Liu, Jun.

Integration of microchip-capillary electrophoresis separation with matrix-assisted laser desorption/ionization (MALDI) Fourier transform mass spectrometry (FTMS) and quantitative determination of oligosaccharide expression levels in individual cells. - 125 p.

Adviser: Calito B. Lebrilla.

Thesis (Ph.D.)--University of California, Davis, 2002.

The first chapter provides a brief introduction of the emergence, development and the significance of the micro-total analysis system (μ-TAS). It also reviews the current application of μ-TAS on biotechnology and pharmaceutical industries. The core of the μ-TAS, micro-device separation, and its principle are explained in this chapter as well.

ISBN: 0493950338Subjects--Topical Terms:

586156
Chemistry, Analytical.

Integration of microchip-capillary electrophoresis separation with matrix-assisted laser desorption/ionization (MALDI) Fourier transform mass spectrometry (FTMS) and quantitative determination of oligosaccharide expression levels in individual cells.
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245 1 0 $a Integration of microchip-capillary electrophoresis separation with matrix-assisted laser desorption/ionization (MALDI) Fourier transform mass spectrometry (FTMS) and quantitative determination of oligosaccharide expression levels in individual cells.
300 $a 125 p.
500 $a Adviser: Calito B. Lebrilla.
500 $a Source: Dissertation Abstracts International, Volume: 63-12, Section: B, page: 5809.
502 $a Thesis (Ph.D.)--University of California, Davis, 2002.
520 $a The first chapter provides a brief introduction of the emergence, development and the significance of the micro-total analysis system (μ-TAS). It also reviews the current application of μ-TAS on biotechnology and pharmaceutical industries. The core of the μ-TAS, micro-device separation, and its principle are explained in this chapter as well.
520 $a The second chapter introduces the integration of micro-device separation with mass spectrometry. It demonstrates a new method developed in this laboratory to couple microchip separation with matrix-assisted laser desorption/ionization Fourier Transform mass spectrometry. The designs of the microchips and a new MALDI ionization source for this coupling are displayed and the new integrated system function as expected. Completed separations of simple biological mixtures were achieved using this integrated system.
520 $a Chapter three describes the design and fabrication of a new multisample probe based on the new MALDI ionization source. The probe is used to analyze tens of samples simultaneously. The experimental results shows that the multisample probe benefits the study of the alkali-metal-coordinated oligosaccharide without sacrificing the sensitivity compared to the single-sample probe.
520 $a Chapter four provides a brief overview of the study of oligosaccharides analysis and the current methods. It also summarizes the previous study of <italic> O</italic>-linked oligosaccharides of <italic>Xenopus laevis</italic>, a South African frog, using MALDI-FT/MS and the Catalog-Library Approach (CLA) that is developed in this laboratory.
520 $a Chapter five demonstrates a new method for quantitative determination of oligosaccharide expression levels using isotope labeling, HPLC separation and MALDI-FT/MS analysis. Using this method, quantitative oligosaccharide expression levels were characterized between individual eggs of the different specimens and of the same specimens as well. Results indicate that oligosaccharides from eggs of different frogs show greater variations. Additionally, minor components show large diversity than major components.
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791 $a Ph.D.
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