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Advances in forensic science: Improv...

University of Virginia.

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Advances in forensic science: Improving sexual assault evidence analysis.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Advances in forensic science: Improving sexual assault evidence analysis./
作者:	Norris, Jessica Voorhees.
面頁冊數:	184 p.
附註:	Adviser: James P. Landers.
Contained By:	Dissertation Abstracts International70-02B.
標題:	Anthropology, Medical and Forensic. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoeng/servlet/advanced?query=3348723
ISBN:	9781109043822

Advances in forensic science: Improving sexual assault evidence analysis.
Norris, Jessica Voorhees.

Advances in forensic science: Improving sexual assault evidence analysis. - 184 p.

Adviser: James P. Landers.

Thesis (Ph.D.)--University of Virginia, 2008.

Genetic analysis of DNA obtained from vaginal swabs is well-established for investigation of sexual assault cases. Although conventional analysis techniques are effective, they are time-consuming and laborious, which has produced a substantial backlog of casework samples to be analyzed. Microfluidic technologies present one solution to this problem. The work presented describes techniques aimed at improving forensic genetic analysis techniques, and specifically addresses procedures necessary for sexual assault evidence analysis, where male and female DNA must be separately interrogated.

ISBN: 9781109043822Subjects--Topical Terms:

1020279
Anthropology, Medical and Forensic.

Advances in forensic science: Improving sexual assault evidence analysis.
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245 1 0 $a Advances in forensic science: Improving sexual assault evidence analysis.
300 $a 184 p.
500 $a Adviser: James P. Landers.
500 $a Source: Dissertation Abstracts International, Volume: 70-02, Section: B, page: 0990.
502 $a Thesis (Ph.D.)--University of Virginia, 2008.
520 $a Genetic analysis of DNA obtained from vaginal swabs is well-established for investigation of sexual assault cases. Although conventional analysis techniques are effective, they are time-consuming and laborious, which has produced a substantial backlog of casework samples to be analyzed. Microfluidic technologies present one solution to this problem. The work presented describes techniques aimed at improving forensic genetic analysis techniques, and specifically addresses procedures necessary for sexual assault evidence analysis, where male and female DNA must be separately interrogated.
520 $a Sexual assault evidence typically contains a mixture of sperm cells from the perpetrator in an excess of epithelial cells from the victim; efficient recovery of cells from the cotton swab, as well as isolation of sperm cells from female DNA, are required for analysis. Conventional methods for recovery and separation of the genetic material are time-consuming and often inefficient. In an effort to improve cell recovery over conventional methods, two procedures were developed and are described; the first incorporates enzymes for digestion of the cotton matrix, while the second includes detergents for the release of cells from cotton, and proteolytic digestion for preferential lysis of epithelial cells. These methods can be used to supplant conventional macroscale techniques, or in conjunction with microchip cell separation methods.
520 $a Microchip technology offers the potential of rapid, cost-effective alternatives to conventional DNA analysis methods. Techniques performed on microdevices are particularly advantageous because they can be integrated with downstream analytical steps on a single microfluidic device in the form of a micro-total analysis system (muTAS). Implementation of integrated systems for forensic DNA analysis will reduce analysis times, and, therefore, the forensic casework backlog. The presented work also details the development of an integrated microdevice for sedimentation-based cell sorting of sperm and epithelial cells, and independent silica-based solid phase extraction of the two cell types. In addition, the development of an alternative cell separation method, based on capture of cells using acoustic trapping, is presented. The work presented represents major steps towards the development of a fully integrated microdevice capable of total DNA analysis for forensic casework.
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