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Bioanalytical methods for investigat...

The University of North Carolina at Chapel Hill., Chemistry.

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Bioanalytical methods for investigating bacterial adhesion and the antibacterial action of nitric oxide.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Bioanalytical methods for investigating bacterial adhesion and the antibacterial action of nitric oxide./
Author:	Deupree, Susan M.
Description:	179 p.
Notes:	Adviser: Mark H. Schoenfisch.
Contained By:	Dissertation Abstracts International70-04B.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoeng/servlet/advanced?query=3352965
ISBN:	9781109113877

Bioanalytical methods for investigating bacterial adhesion and the antibacterial action of nitric oxide.
Deupree, Susan M.

Bioanalytical methods for investigating bacterial adhesion and the antibacterial action of nitric oxide. - 179 p.

Adviser: Mark H. Schoenfisch.

Thesis (Ph.D.)--The University of North Carolina at Chapel Hill, 2009.

Infection is a continuing problem in both hospital and community settings, further compounded by swift adaptation and rising emergence of more virulent and antibacterial-resistant pathogens. The complex mechanisms underlying the process of infection must be understood in order to develop preventative technologies. Simultaneously, effective solutions must be devised to counter infections as they arise. My dissertation research has contributed to both aspects by working to understand the process of adhesion as well as the development of novel therapeutic strategies.

ISBN: 9781109113877Subjects--Topical Terms:

1017734
Biology, Microbiology.

Bioanalytical methods for investigating bacterial adhesion and the antibacterial action of nitric oxide.
LDR:03781nam 2200385 a 45 001 857131
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020 $a 9781109113877
035 $a (UMI)AAI3352965
035 $a AAI3352965
040 $a UMI $c UMI
100 1 $a Deupree, Susan M. $3 1024063
245 1 0 $a Bioanalytical methods for investigating bacterial adhesion and the antibacterial action of nitric oxide.
300 $a 179 p.
500 $a Adviser: Mark H. Schoenfisch.
500 $a Source: Dissertation Abstracts International, Volume: 70-04, Section: B, page: 2278.
502 $a Thesis (Ph.D.)--The University of North Carolina at Chapel Hill, 2009.
520 $a Infection is a continuing problem in both hospital and community settings, further compounded by swift adaptation and rising emergence of more virulent and antibacterial-resistant pathogens. The complex mechanisms underlying the process of infection must be understood in order to develop preventative technologies. Simultaneously, effective solutions must be devised to counter infections as they arise. My dissertation research has contributed to both aspects by working to understand the process of adhesion as well as the development of novel therapeutic strategies.
520 $a To investigate bacterial adhesion at a fundamental level, a quantitative method was developed for measuring the shear force required to detach individual adhered bacteria using atomic force microscopy (AFM) that featured both improved accuracy and higher-throughput data acquisition. This technique was employed to characterize the adhesion strength kinetics of Pseudomonas aeruginosa and Staphylococcus aureus. Both the magnitudes of detachment force and rates of increase in adhesion strength were greater for P. aeruginosa than for S. aureus. Furthermore, adhered cells demonstrated a range of adhesion forces that broadened with time, indicating that change in adhesion strength does not proceed uniformly.
520 $a Morphological analyses were conducted to study the antimicrobial properties of nitric oxide (NO) against two Gram-negative pathogens. The effects of NO as a function of concentration, exposure time, and delivery format were studied using two materials with differing NO-release properties. Analysis of cell topography revealed that higher doses of NO correlated with increasing membrane roughness. Treatment with amoxicillin, an antibiotic that compromises the integrity of the cell wall, led to morphologies resembling those resulting from NO treatment. Our observations indicated cell wall deterioration is a consequence of NO-exposure for both species studied.
520 $a The combination of NO and silver sulfadiazine (AgSD) was evaluated for bactericidal efficacy using a modified broth microdilution technique and a checkerboard-type assay. The combination of NO and AgSD proved synergistic against most pathogens, particularly the Gram-positive species. A highly synergistic effect was produced against S. aureus and E. faecalis , including a vancomycin-resistant strain. The synergistic activity of AgSD and NO against a broad range of pathogens advocates future investigation of this therapeutic combination for use as a topical anti-infective.
590 $a School code: 0153.
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Chemistry, Biochemistry. $3 1017722
650 4 $a Chemistry, Pharmaceutical. $3 550957
690 $a 0410
690 $a 0487
690 $a 0491
710 2 $a The University of North Carolina at Chapel Hill. $b Chemistry. $3 1021872
773 0 $t Dissertation Abstracts International $g 70-04B.
790 $a 0153
790 1 0 $a Falvo, Michael $e committee member
790 1 0 $a Lord, Susan $e committee member
790 1 0 $a Schoenfisch, Mark H., $e advisor
790 1 0 $a Schoenfisch, Mark $e committee member
790 1 0 $a Sheiko, Sergei $e committee member
790 1 0 $a Spremulli, Linda $e committee member
791 $a Ph.D.
792 $a 2009
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoeng/servlet/advanced?query=3352965