東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Development, characterization, and a...

The University of Arizona., Physiological Sciences.

Linked to FindBook

Google Book

Amazon

博客來

Development, characterization, and assessment of a tissue-engineered prevascularized pancreatic islet encapsulation device.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Development, characterization, and assessment of a tissue-engineered prevascularized pancreatic islet encapsulation device./
Author:	Hiscox, Alton Michael.
Description:	165 p.
Notes:	Adviser: Stuart K. Williams.
Contained By:	Dissertation Abstracts International69-02B.
Subject:	Biology, Cell. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoeng/servlet/advanced?query=3297238
ISBN:	9780549450702

Development, characterization, and assessment of a tissue-engineered prevascularized pancreatic islet encapsulation device.
Hiscox, Alton Michael.

Development, characterization, and assessment of a tissue-engineered prevascularized pancreatic islet encapsulation device. - 165 p.

Adviser: Stuart K. Williams.

Thesis (Ph.D.)--The University of Arizona, 2008.

Islet transplantation for the purpose of treating insulin-dependent diabetes is currently limited by several factors, most significantly, islet survival post transplantation. In the following dissertation, a tissue-engineered prevascularized pancreatic encapsulating device (PPED) was designed, developed, and evaluated. Microvessel fragments placed within a 3-dimensional collagen-based matrix produce and secrete vascular endothelial growth factor, and inosculate with the host circulation. Isolated islets placed within collagen gels exhibited four-fold more insulin release in response to glucose stimulation than islets in tissue culture. The insulin released by beta-cells in islets encapsulated in collagen exhibited unobstructed diffusion within the collagen gels. Subsequent studies evaluated the ability to create a sandwich comprised of two layers of prevascularized collagen gels around a central collagen gel containing islets. In vitro characterization of the islets within these constructs showed that islets are functional and respond to glucose stimulation. The PPEDs were implanted subcutaneously into SCID mice. Islet survival was assessed after 7, 14, and 28 days. Immunohistochemical analysis was performed on the implants to detect insulin and the presence of intraislet endothelial cells. At all time points, insulin was localized in association with intact and partially dissociated islets. Moreover, cells that exhibited insulin staining were co-localized with intraislet endothelial cells. Lastly, dextran-perfused PPEDs showed host perfusion throughout the implant, including perfusion to structures that are morphologically consistent with pancreatic islets. These data indicate that the PPED enhances islet survival by supporting islet viability, by maintaining intraislet endothelial cells, and by enhancing reperfusion to the islets.

ISBN: 9780549450702Subjects--Topical Terms:

1017686
Biology, Cell.

Development, characterization, and assessment of a tissue-engineered prevascularized pancreatic islet encapsulation device.
LDR:02821nam 2200289 a 45 001 856641
005 20100709
008 100709s2008 ||||||||||||||||| ||eng d
020 $a 9780549450702
035 $a (UMI)AAI3297238
035 $a AAI3297238
040 $a UMI $c UMI
100 1 $a Hiscox, Alton Michael. $3 1023473
245 1 0 $a Development, characterization, and assessment of a tissue-engineered prevascularized pancreatic islet encapsulation device.
300 $a 165 p.
500 $a Adviser: Stuart K. Williams.
500 $a Source: Dissertation Abstracts International, Volume: 69-02, Section: B, page: 0864.
502 $a Thesis (Ph.D.)--The University of Arizona, 2008.
520 $a Islet transplantation for the purpose of treating insulin-dependent diabetes is currently limited by several factors, most significantly, islet survival post transplantation. In the following dissertation, a tissue-engineered prevascularized pancreatic encapsulating device (PPED) was designed, developed, and evaluated. Microvessel fragments placed within a 3-dimensional collagen-based matrix produce and secrete vascular endothelial growth factor, and inosculate with the host circulation. Isolated islets placed within collagen gels exhibited four-fold more insulin release in response to glucose stimulation than islets in tissue culture. The insulin released by beta-cells in islets encapsulated in collagen exhibited unobstructed diffusion within the collagen gels. Subsequent studies evaluated the ability to create a sandwich comprised of two layers of prevascularized collagen gels around a central collagen gel containing islets. In vitro characterization of the islets within these constructs showed that islets are functional and respond to glucose stimulation. The PPEDs were implanted subcutaneously into SCID mice. Islet survival was assessed after 7, 14, and 28 days. Immunohistochemical analysis was performed on the implants to detect insulin and the presence of intraislet endothelial cells. At all time points, insulin was localized in association with intact and partially dissociated islets. Moreover, cells that exhibited insulin staining were co-localized with intraislet endothelial cells. Lastly, dextran-perfused PPEDs showed host perfusion throughout the implant, including perfusion to structures that are morphologically consistent with pancreatic islets. These data indicate that the PPED enhances islet survival by supporting islet viability, by maintaining intraislet endothelial cells, and by enhancing reperfusion to the islets.
590 $a School code: 0009.
650 4 $a Biology, Cell. $3 1017686
650 4 $a Biology, Physiology. $3 1017816
650 4 $a Engineering, Biomedical. $3 1017684
690 $a 0379
690 $a 0541
690 $a 0719
710 2 $a The University of Arizona. $b Physiological Sciences. $3 1023472
773 0 $t Dissertation Abstracts International $g 69-02B.
790 $a 0009
790 1 0 $a Williams, Stuart K., $e advisor
791 $a Ph.D.
792 $a 2008
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoeng/servlet/advanced?query=3297238