東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Autologous bone marrow-derived mesen...

University of Missouri - Columbia.

Linked to FindBook

Google Book

Amazon

博客來

Autologous bone marrow-derived mesenchymal stem cell transplantation as a therapy for neuronal ceroid lipofuscinosis.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Autologous bone marrow-derived mesenchymal stem cell transplantation as a therapy for neuronal ceroid lipofuscinosis./
Author:	Sanders, Douglas N.
Description:	178 p.
Notes:	Adviser: Martin L. Katz.
Contained By:	Dissertation Abstracts International70-03B.
Subject:	Biology, Cell. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3351662
ISBN:	9781109080247

Autologous bone marrow-derived mesenchymal stem cell transplantation as a therapy for neuronal ceroid lipofuscinosis.
Sanders, Douglas N.

Autologous bone marrow-derived mesenchymal stem cell transplantation as a therapy for neuronal ceroid lipofuscinosis. - 178 p.

Adviser: Martin L. Katz.

Thesis (Ph.D.)--University of Missouri - Columbia, 2007.

The Neuronal Ceroid Lipofuscinoses (NCLs) are a group of rare genetic diseases characterized by neurodegeneration and accumulation of autofluorescent lysosomal storage bodies in certain cells. There is currently no effective clinical treatment for the NCLs, though research indicates that stem cell transplant may be efficacious. The bone marrow stroma is composed of a heterogeneous population of cells including endothelial cells, fibroblasts, adipocytes, osteocytes and two types of multipotent adult stem cells. Mesenchymal stem cells (MSCs) are stroma-derived adult stem cells which are known to give rise to cells of mesodermal origin including bone, cartilage, fat, tendon, muscle and possibly germ cells. MSCs may also be capable of differentiating into cells of a non-mesodermal origin, including neural cells. We have isolated a population of untransformed, self-renewing, multipotent, stroma-derived cells from both murine and canine bone marrow. These cells express protein markers associated with MSCs, grows quickly in culture, with a doubling time of 24-36 hours, and murine cells have been expanded in culture for more than 150 population doublings without evidence of senescence or loss of differentiation potential. Upon differentiation, these MSCs express appropriate protein and RNA markers and display histochemical features consistent with terminally differentiated cells.

ISBN: 9781109080247Subjects--Topical Terms:

1017686
Biology, Cell.

Autologous bone marrow-derived mesenchymal stem cell transplantation as a therapy for neuronal ceroid lipofuscinosis.
LDR:04356nam 2200325 a 45 001 855284
005 20100708
008 100708s2007 ||||||||||||||||| ||eng d
020 $a 9781109080247
035 $a (UMI)AAI3351662
035 $a AAI3351662
040 $a UMI $c UMI
100 1 $a Sanders, Douglas N. $3 1021854
245 1 0 $a Autologous bone marrow-derived mesenchymal stem cell transplantation as a therapy for neuronal ceroid lipofuscinosis.
300 $a 178 p.
500 $a Adviser: Martin L. Katz.
500 $a Source: Dissertation Abstracts International, Volume: 70-03, Section: B, page: 1513.
502 $a Thesis (Ph.D.)--University of Missouri - Columbia, 2007.
520 $a The Neuronal Ceroid Lipofuscinoses (NCLs) are a group of rare genetic diseases characterized by neurodegeneration and accumulation of autofluorescent lysosomal storage bodies in certain cells. There is currently no effective clinical treatment for the NCLs, though research indicates that stem cell transplant may be efficacious. The bone marrow stroma is composed of a heterogeneous population of cells including endothelial cells, fibroblasts, adipocytes, osteocytes and two types of multipotent adult stem cells. Mesenchymal stem cells (MSCs) are stroma-derived adult stem cells which are known to give rise to cells of mesodermal origin including bone, cartilage, fat, tendon, muscle and possibly germ cells. MSCs may also be capable of differentiating into cells of a non-mesodermal origin, including neural cells. We have isolated a population of untransformed, self-renewing, multipotent, stroma-derived cells from both murine and canine bone marrow. These cells express protein markers associated with MSCs, grows quickly in culture, with a doubling time of 24-36 hours, and murine cells have been expanded in culture for more than 150 population doublings without evidence of senescence or loss of differentiation potential. Upon differentiation, these MSCs express appropriate protein and RNA markers and display histochemical features consistent with terminally differentiated cells.
520 $a Using a suicide gene knockout model, we demonstrate genetic modification of our MSC isolate through small fragment homologous replacement (SFHR) as a precursor to repairing NCL-specific genes in autologous cells. Intraocular injection of eGFP expressing murine MSCs into the eye of a murine model of human infantile NCL (INCL) show that transplanted MSCs are capable of surviving in the eye of an allogeneic host for up to 5 weeks. Withing 2 weeks after injection, cells were observed to form close associations with the retinal tissue and at 5 weeks, MSCs had integrated into host tissue with no obvious signs of immune rejection. We also report that MSCs transplanted onto brain slices cultured from NCL-affected mice are able to reduce the amount of autofluorescent storage material in surrounding cells through enzymatic cross-correction. Isolation of canine MSCs and characterization of several models of canine NCL lay the foundation for similar experiments in canine models.
520 $a Our characterization of these cells indicates they may be ideal candidates for studying autologous adult stem cell transplants as a therapy in patients with neurodegenerative disease. Further experiments in animal model systems will help to determine the most effective method of implantation, the potential for cellular replacement and survival time of donor cells after transplant. Because of their multipotent phenotype, ease of isolation and growth in culture, ready ability to engraft in a multitude of tissues and susceptibility to genetic manipulation, autologous MSCs offer many advantages over other potential therapies as a treatment for NCLs. Development of a standardized approach to using autologous MSC transplant as a therapy could lead to vast improvements in clinical outcome not just for NCLs but for numerous other diseases including common ailments such as heart disease, stroke, diabetes and Parkinson's as well as other rare inherited genetic disorders.
590 $a School code: 0133.
650 4 $a Biology, Cell. $3 1017686
650 4 $a Biology, Neuroscience. $3 1017680
650 4 $a Health Sciences, Ophthalmology. $3 1019445
650 4 $a Health Sciences, Pathology. $3 1017854
690 $a 0317
690 $a 0379
690 $a 0381
690 $a 0571
710 2 $a University of Missouri - Columbia. $3 1017522
773 0 $t Dissertation Abstracts International $g 70-03B.
790 $a 0133
790 1 0 $a Katz, Martin L., $e advisor
791 $a Ph.D.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3351662