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Consequences of post-ejaculatory str...

University of Florida.

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Consequences of post-ejaculatory stress on the bovine spermatozoa for the resultant embryo.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Consequences of post-ejaculatory stress on the bovine spermatozoa for the resultant embryo./
作者:	Hendricks, Katherine E. M.
面頁冊數:	132 p.
附註:	Adviser: Peter J. Hansen.
Contained By:	Dissertation Abstracts International70-02B.
標題:	Biology, Animal Physiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3347134
ISBN:	9781109024579

Consequences of post-ejaculatory stress on the bovine spermatozoa for the resultant embryo.
Hendricks, Katherine E. M.

Consequences of post-ejaculatory stress on the bovine spermatozoa for the resultant embryo. - 132 p.

Adviser: Peter J. Hansen.

Thesis (Ph.D.)--University of Florida, 2008.

The ability of the preimplantation embryo to complete its developmental program is determined in large part by its acquisition of genetic and non-genetic components from the sperm and oocyte. While the role of damage to the oocyte on embryo competence has been well established, it is not clear whether damage to the sperm after ejaculation results in formation of an embryo with reduced competence for development. Accordingly, a series of experiments were conducted to determine whether this was the case. Stresses examined were aging, heat shock, irradiation, and oxidative stress. The experimental approach involved evaluation of oocytes inseminated with sperm exposed to stress to cleave and develop to the blastocyst stage of development.

ISBN: 9781109024579Subjects--Topical Terms:

1017835
Biology, Animal Physiology.

Consequences of post-ejaculatory stress on the bovine spermatozoa for the resultant embryo.
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100 1 $a Hendricks, Katherine E. M. $3 1021832
245 1 0 $a Consequences of post-ejaculatory stress on the bovine spermatozoa for the resultant embryo.
300 $a 132 p.
500 $a Adviser: Peter J. Hansen.
500 $a Source: Dissertation Abstracts International, Volume: 70-02, Section: B, page: 0771.
502 $a Thesis (Ph.D.)--University of Florida, 2008.
520 $a The ability of the preimplantation embryo to complete its developmental program is determined in large part by its acquisition of genetic and non-genetic components from the sperm and oocyte. While the role of damage to the oocyte on embryo competence has been well established, it is not clear whether damage to the sperm after ejaculation results in formation of an embryo with reduced competence for development. Accordingly, a series of experiments were conducted to determine whether this was the case. Stresses examined were aging, heat shock, irradiation, and oxidative stress. The experimental approach involved evaluation of oocytes inseminated with sperm exposed to stress to cleave and develop to the blastocyst stage of development.
520 $a Aging of sperm by incubation for 4 h at a temperature characteristic of normal body temperature (38.5 degree Celsius) or hyperthermia (40 degree Celsius) reduced cleavage, but did not alter embryo competence as measured by the percent of cleaved embryos that became blastocysts. Similarly, exposure of sperm to X-irradiation characteristic of airport screening devices for checked or carry-on luggage had no effect on embryo competence. Cleavage rate was reduced by X-irradiation after multiple exposures at the checked luggage dose but there was no reduction in the percent of cleaved embryos becoming blastocysts at any exposure.
520 $a In contrast, oxidative stress did affect embryo competence to develop to the blastocyst stage. Oocytes inseminated with sperm treated with menadione or tert-butyl hydroperoxide had reduced cleavage and a reduced percentage of cleaved embryos that became blastocysts.
520 $a One potential mechanism for sperm damage affecting the resultant embryo is the initiation of DNA damage during sperm cell apoptosis. To test whether apoptosis could be induced in ejaculated spermatozoa, bull and stallion sperm were subjected to heat shock and aging and features of apoptosis evaluated. Endpoints included terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), mitochondrial membrane potential, and presence and activation of procaspase-9 and procaspase-3. There was only a slight increase in TUNEL labeling after 24 h aging and the increase was not blocked with a caspase inhibitor. Moreover, procaspase-9 was detected in bovine sperm but not activated by 4 h aging at 38.5, 40 and 41 degree Celsius and procaspase-3 was not detected.
520 $a Taken together, these results indicate that specific types of stress occurring after ejaculation can negatively impact the ability of the subsequent embryo to develop. Understanding these effects on early embryonic development may lead to new approaches for reducing early embryonic loss.
590 $a School code: 0070.
650 4 $a Biology, Animal Physiology. $3 1017835
690 $a 0433
710 2 $a University of Florida. $3 718949
773 0 $t Dissertation Abstracts International $g 70-02B.
790 $a 0070
790 1 0 $a Hansen, Peter J., $e advisor
791 $a Ph.D.
792 $a 2008
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3347134