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Microsatellite DNA analysis of four ...

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Microsatellite DNA analysis of four Culex pipiens quinquefasciatus (Say) (Diptera: Culicidae) mosquito populations in Florida and their vector competence for West Nile virus.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Microsatellite DNA analysis of four Culex pipiens quinquefasciatus (Say) (Diptera: Culicidae) mosquito populations in Florida and their vector competence for West Nile virus./
Author:	Doumbouya, Aissa Evelyn.
Description:	274 p.
Notes:	Advisers: James E. Maruniak; Jonathan F. Day.
Contained By:	Dissertation Abstracts International69-04B.
Subject:	Biology, Entomology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3311624
ISBN:	9780549606215

Microsatellite DNA analysis of four Culex pipiens quinquefasciatus (Say) (Diptera: Culicidae) mosquito populations in Florida and their vector competence for West Nile virus.
Doumbouya, Aissa Evelyn.

Microsatellite DNA analysis of four Culex pipiens quinquefasciatus (Say) (Diptera: Culicidae) mosquito populations in Florida and their vector competence for West Nile virus. - 274 p.

Advisers: James E. Maruniak; Jonathan F. Day.

Thesis (Ph.D.)--University of Florida, 2007.

Since its introduction to New York in 1999, West Nile virus (WNV) has spread across the USA following bird migration routes. Many studies have been published studying the potential risk to humans, birds, horses, and wildlife. However, none have been conducted to look at the unique climate of Florida where WNV-related bird deaths, horse cases and human morbidity was first reported in 2001. The research presented here will provide a laboratory-based approach to evaluating the vector competence of Cx. p. quinquefasciatus in Florida for WNV infection. Culex pipiens quinquefasciatus mosquitoes are opportunistic feeders that bite both humans and birds. Studies in California have shown that Culex mosquitoes differ in their vector competence based on their geographical range. The work reported here sought to elucidate the unique properties of WNV infection as it pertained to Cx. p. quinquefasciatus and its vector competence to WNV in Florida. A strain of WNV (WN-FL03) was isolated from Culex nigripalpus females collected in Indian River County in 2003. It was used to infect newly colonized Cx. p. quinquefasciatus mosquitoes from Walton, Manatee, and Alachua counties, and an 11 year-old captive colony which was also from Alachua County. The Indian River County WNV isolate was sequenced and compared to the WNV prototype that was isolated in NY in 1999. The infection rates of the different geographical strains from Florida ranged from 27.5% (11/40) to 54.4% (31/57) on day seven and 31.6% (12/38) to 66.0% (35/53) on day 14. The dissemination rates ranged from 1.8% (1/57) to 13.6% (6/44) on day seven and 23.7% (9/38) to 39.6% (35/53) on day 14. The four Cx. p. quinquefasciatus populations were also compared using four microsatellite markers to determine their genetic differences and similarities. It was determined that all of the mosquito strains were genetically different based on comparison of microsatellite loci. However, no trends were seen to suggest that the genetic difference influenced the differences in the WNV infection and dissemination rates for the four mosquito strains. Possible implications of these observations are discussed in the following chapters.

ISBN: 9780549606215Subjects--Topical Terms:

1018619
Biology, Entomology.

Microsatellite DNA analysis of four Culex pipiens quinquefasciatus (Say) (Diptera: Culicidae) mosquito populations in Florida and their vector competence for West Nile virus.
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100 1 $a Doumbouya, Aissa Evelyn. $3 1018694
245 1 0 $a Microsatellite DNA analysis of four Culex pipiens quinquefasciatus (Say) (Diptera: Culicidae) mosquito populations in Florida and their vector competence for West Nile virus.
300 $a 274 p.
500 $a Advisers: James E. Maruniak; Jonathan F. Day.
500 $a Source: Dissertation Abstracts International, Volume: 69-04, Section: B, page: 2069.
502 $a Thesis (Ph.D.)--University of Florida, 2007.
520 $a Since its introduction to New York in 1999, West Nile virus (WNV) has spread across the USA following bird migration routes. Many studies have been published studying the potential risk to humans, birds, horses, and wildlife. However, none have been conducted to look at the unique climate of Florida where WNV-related bird deaths, horse cases and human morbidity was first reported in 2001. The research presented here will provide a laboratory-based approach to evaluating the vector competence of Cx. p. quinquefasciatus in Florida for WNV infection. Culex pipiens quinquefasciatus mosquitoes are opportunistic feeders that bite both humans and birds. Studies in California have shown that Culex mosquitoes differ in their vector competence based on their geographical range. The work reported here sought to elucidate the unique properties of WNV infection as it pertained to Cx. p. quinquefasciatus and its vector competence to WNV in Florida. A strain of WNV (WN-FL03) was isolated from Culex nigripalpus females collected in Indian River County in 2003. It was used to infect newly colonized Cx. p. quinquefasciatus mosquitoes from Walton, Manatee, and Alachua counties, and an 11 year-old captive colony which was also from Alachua County. The Indian River County WNV isolate was sequenced and compared to the WNV prototype that was isolated in NY in 1999. The infection rates of the different geographical strains from Florida ranged from 27.5% (11/40) to 54.4% (31/57) on day seven and 31.6% (12/38) to 66.0% (35/53) on day 14. The dissemination rates ranged from 1.8% (1/57) to 13.6% (6/44) on day seven and 23.7% (9/38) to 39.6% (35/53) on day 14. The four Cx. p. quinquefasciatus populations were also compared using four microsatellite markers to determine their genetic differences and similarities. It was determined that all of the mosquito strains were genetically different based on comparison of microsatellite loci. However, no trends were seen to suggest that the genetic difference influenced the differences in the WNV infection and dissemination rates for the four mosquito strains. Possible implications of these observations are discussed in the following chapters.
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773 0 $t Dissertation Abstracts International $g 69-04B.
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790 1 0 $a Maruniak, James E., $e advisor
791 $a Ph.D.
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