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RNA silencing : = methods and protoc...

Carmichael, Gordon.

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RNA silencing : = methods and protocols /

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	RNA silencing :/ edited by Gordon Carmichael.
其他題名:	methods and protocols /
其他作者:	Carmichael, Gordon.
出版者:	Totowa, N.J. :Humana Press, : c2005.,
面頁冊數:	xi, 338 p. :ill. ;24 cm.
內容註:	Identification of components of RNAi pathways using the tandem affinity purification method / Mikiko C. Siomi and Haruhiko Siomi -- Separation of drosophila RNA silencing complexes by native gel electrophoresis/ John W. Pham and Erik J. Sontheimer -- Preparation and analysis of drosha / Yoontae Lee and V. Narry Kim -- RNA interference in Caenorhabditis elegans/ Nathaniel R. Dudley and Bob Goldstein -- Down-regulating gene expression by RNA interference in Trypanosoma brucei/ Christine E. Clayton ... [et al.] -- Analysis of double-stranded RNA and small RNAs involved in RNA-mediated transcriptional gene silencing/ M. Florian Mette ... [et al.] -- Strategies for the design of random siRNA libraries and the selection of anti-GFP siRNAs / Mouldy Sioud and Sebastiona Wulchli -- Silencing gene expression with dicer-generated siRNA pools/ Jason W. Myers and James E. Ferrell, Jr.
內容註:	Inhibition of gene expression in vivo using multiplex siRNA / Sung-Suk Chae and Timothy Hla -- Gene silencing by a DNA vector-based RNAi technology/ Guangchao Sui and Yang Shi -- Plasmid-based RNA interference: construction of small-hairpin RNA expression vectors / Scott Q. Harper and Beverly L. Davidson -- SiRNA delivery in vivo/ Mouldy Sioud -- Peptide-based strategy for siRNA delivery into mammalian cells / Federica Simeoni ... [et al.] -- Lentiviral vector delivery of siRNA and shRNA encoding genes into cultured and primary hematopoietic cells/ Mingjie Li and Jo Rossi -- Exon-specific RNA interference: a tool to determine the functional relevance of proteins encoded by alternatively spliced mRNAs/ Alicia M. Celotto, Joo-Won Lee, and Brenton R. Graveley -- Immunoprecipitation of microRNPs and directional cloning of microRNAs / Elsa Maniataki, Maria Dels Angels de Planell Saguer, and Zissimos Mourelatos --.
內容註:	Detection of microRNAs and assays to monitor microRNA activities in vivo and in vitro / Marianthi Kiriakidou ... [et al.] -- High throughput analysis of microRNA gene expression using sensitive cDNA probes/ Mouldy Sioud and Oystein Rosok -- Modification of human U1 snRNA for inhibition of gene expression at the level of pre-mRNA / Peng Liu ... [et al.].
標題:	Gene silencing - Laboratory manuals. -
電子資源:	http://www.loc.gov/catdir/toc/ecip051/2004023107.htmlhttp://www.loc.gov/catdir/toc/ecip051/2004023107.html
ISBN:	1588294366 :

RNA silencing : = methods and protocols /
RNA silencing :methods and protocols /edited by Gordon Carmichael. - Totowa, N.J. :Humana Press,c2005. - xi, 338 p. :ill. ;24 cm. - Methods in molecular biology ;309.. - Methods in molecular biology (Clifton, N.J.) ;v. 309..

Includes bibliographical references and index.

Identification of components of RNAi pathways using the tandem affinity purification method / Mikiko C. Siomi and Haruhiko Siomi -- Separation of drosophila RNA silencing complexes by native gel electrophoresis/ John W. Pham and Erik J. Sontheimer -- Preparation and analysis of drosha / Yoontae Lee and V. Narry Kim -- RNA interference in Caenorhabditis elegans/ Nathaniel R. Dudley and Bob Goldstein -- Down-regulating gene expression by RNA interference in Trypanosoma brucei/ Christine E. Clayton ... [et al.] -- Analysis of double-stranded RNA and small RNAs involved in RNA-mediated transcriptional gene silencing/ M. Florian Mette ... [et al.] -- Strategies for the design of random siRNA libraries and the selection of anti-GFP siRNAs / Mouldy Sioud and Sebastiona Wulchli -- Silencing gene expression with dicer-generated siRNA pools/ Jason W. Myers and James E. Ferrell, Jr.

ISBN: 1588294366 :US125.00

LCCN: 2004023107Subjects--Topical Terms:

753201
Gene silencing
--Laboratory manuals.

LC Class. No.: QP623.5.S63 / R634 2005

Dewey Class. No.: 572.8/65

National Library of Medicine Call No.: QU 58.7 / R6273 2005

RNA silencing : = methods and protocols /
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505 0 $a Inhibition of gene expression in vivo using multiplex siRNA / Sung-Suk Chae and Timothy Hla -- Gene silencing by a DNA vector-based RNAi technology/ Guangchao Sui and Yang Shi -- Plasmid-based RNA interference: construction of small-hairpin RNA expression vectors / Scott Q. Harper and Beverly L. Davidson -- SiRNA delivery in vivo/ Mouldy Sioud -- Peptide-based strategy for siRNA delivery into mammalian cells / Federica Simeoni ... [et al.] -- Lentiviral vector delivery of siRNA and shRNA encoding genes into cultured and primary hematopoietic cells/ Mingjie Li and Jo Rossi -- Exon-specific RNA interference: a tool to determine the functional relevance of proteins encoded by alternatively spliced mRNAs/ Alicia M. Celotto, Joo-Won Lee, and Brenton R. Graveley -- Immunoprecipitation of microRNPs and directional cloning of microRNAs / Elsa Maniataki, Maria Dels Angels de Planell Saguer, and Zissimos Mourelatos --.
505 0 $a Detection of microRNAs and assays to monitor microRNA activities in vivo and in vitro / Marianthi Kiriakidou ... [et al.] -- High throughput analysis of microRNA gene expression using sensitive cDNA probes/ Mouldy Sioud and Oystein Rosok -- Modification of human U1 snRNA for inhibition of gene expression at the level of pre-mRNA / Peng Liu ... [et al.].
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650 $a Small interfering RNA $v Laboratory manuals. $3 753202
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650 $a Gene Expression Regulation $x genetics. $3 753204
650 $a MicroRNAs $x physiology. $3 753205
650 $a RNA, Small Interfering $x physiology. $3 753206
700 $a Carmichael, Gordon. $3 753200
830 $a Methods in molecular biology (Clifton, N.J.) ; $v v. 309. $3 753207
856 4 1 $3 Table of contents $u http://www.loc.gov/catdir/toc/ecip051/2004023107.html $z http://www.loc.gov/catdir/toc/ecip051/2004023107.html