東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Inoculum Dose and Sepsis Comorbidity...

Silva, Elvia Elizabeth.

Linked to FindBook

Google Book

Amazon

博客來

Inoculum Dose and Sepsis Comorbidity Alter Disease Outcomes in an MHV-1 Murine Model of Beta-Coronavirus Infections.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Inoculum Dose and Sepsis Comorbidity Alter Disease Outcomes in an MHV-1 Murine Model of Beta-Coronavirus Infections./
Author:	Silva, Elvia Elizabeth.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2024,
Description:	152 p.
Notes:	Source: Dissertations Abstracts International, Volume: 86-01, Section: B.
Contained By:	Dissertations Abstracts International86-01B.
Subject:	Immunology. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31148610
ISBN:	9798383188279

Inoculum Dose and Sepsis Comorbidity Alter Disease Outcomes in an MHV-1 Murine Model of Beta-Coronavirus Infections.
Silva, Elvia Elizabeth.

Inoculum Dose and Sepsis Comorbidity Alter Disease Outcomes in an MHV-1 Murine Model of Beta-Coronavirus Infections. - Ann Arbor : ProQuest Dissertations & Theses, 2024 - 152 p.

Source: Dissertations Abstracts International, Volume: 86-01, Section: B.

Thesis (Ph.D.)--The University of Iowa, 2024.

Immune responses against respiratory viruses have been studied in mouse models for decades, providing insight into the players involved in protection. However, it is also known that patients in the clinic can come in with different comorbidities which make infection worse. Additionally, although exposure length has been studied in the SARS-CoV-2 context, whether the immune response differs when initially exposed to different amounts of coronavirus, has been understudied. In this work I address how sepsis, an immune disorder, and the initial infection amount determine the outcome in beta-coronavirus infection using the MHV-1 mouse model.I noted that in a host whose genetics already predisposes them to severe infection with beta coronavirus, there is a tipping point of asymptomatic to symptomatic and severe infection depending on how much initial virus they receive. This is a different assessment than what is tested in the lungs several days in, which can be much more similar between inoculum doses. A low dose infection was asymptomatic in coronavirus-susceptible mice. While mice had active virus in their lungs and their adaptive immune was responding, they did not experience any outward symptoms and all of them survived. This low dose infection also worked to protect these mice from severe infection later. Specifically, the antibodies generated from the asymptomatic infection protected these mice from a lethal infection. All mice survived and had no detectible virus early after infection.Finally, I addressed the clinically relevant model of prior sepsis as a beta coronavirus infection comorbidity. Hosts who were otherwise resistant to infection due to genetics or initial infection level were worse off if they experienced sepsis before, including poorer morbidity and higher viral load. This thesis covers the alterations in pathology and immune responses after these infection alterations and closes with future directions in immune memory after sepsis.

ISBN: 9798383188279Subjects--Topical Terms:

611031
Immunology.
Subjects--Index Terms:

Coronavirus

Inoculum Dose and Sepsis Comorbidity Alter Disease Outcomes in an MHV-1 Murine Model of Beta-Coronavirus Infections.
LDR:03132nmm a2200373 4500 001 2398598
005 20240812064716.5
006 m o d
007 cr#unu||||||||
008 251215s2024 ||||||||||||||||| ||eng d
020 $a 9798383188279
035 $a (MiAaPQ)AAI31148610
035 $a AAI31148610
040 $a MiAaPQ $c MiAaPQ
100 1 $a Silva, Elvia Elizabeth. $3 3768519
245 1 0 $a Inoculum Dose and Sepsis Comorbidity Alter Disease Outcomes in an MHV-1 Murine Model of Beta-Coronavirus Infections.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2024
300 $a 152 p.
500 $a Source: Dissertations Abstracts International, Volume: 86-01, Section: B.
500 $a Advisor: Badovinac, Vladimir.
502 $a Thesis (Ph.D.)--The University of Iowa, 2024.
520 $a Immune responses against respiratory viruses have been studied in mouse models for decades, providing insight into the players involved in protection. However, it is also known that patients in the clinic can come in with different comorbidities which make infection worse. Additionally, although exposure length has been studied in the SARS-CoV-2 context, whether the immune response differs when initially exposed to different amounts of coronavirus, has been understudied. In this work I address how sepsis, an immune disorder, and the initial infection amount determine the outcome in beta-coronavirus infection using the MHV-1 mouse model.I noted that in a host whose genetics already predisposes them to severe infection with beta coronavirus, there is a tipping point of asymptomatic to symptomatic and severe infection depending on how much initial virus they receive. This is a different assessment than what is tested in the lungs several days in, which can be much more similar between inoculum doses. A low dose infection was asymptomatic in coronavirus-susceptible mice. While mice had active virus in their lungs and their adaptive immune was responding, they did not experience any outward symptoms and all of them survived. This low dose infection also worked to protect these mice from severe infection later. Specifically, the antibodies generated from the asymptomatic infection protected these mice from a lethal infection. All mice survived and had no detectible virus early after infection.Finally, I addressed the clinically relevant model of prior sepsis as a beta coronavirus infection comorbidity. Hosts who were otherwise resistant to infection due to genetics or initial infection level were worse off if they experienced sepsis before, including poorer morbidity and higher viral load. This thesis covers the alterations in pathology and immune responses after these infection alterations and closes with future directions in immune memory after sepsis.
590 $a School code: 0096.
650 4 $a Immunology. $3 611031
650 4 $a Cellular biology. $3 3172791
650 4 $a Pathology. $3 643180
653 $a Coronavirus
653 $a Sepsis
653 $a Immune memory
653 $a Asymptomatic infection
690 $a 0982
690 $a 0379
690 $a 0571
710 2 $a The University of Iowa. $b Immunology. $3 3194029
773 0 $t Dissertations Abstracts International $g 86-01B.
790 $a 0096
791 $a Ph.D.
792 $a 2024
793 $a English
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31148610