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Development of a Xenograft for Annul...

Atsu, Prince Mensah Kwaku.

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Development of a Xenograft for Annular Repair Using Pulsed Electric Field Exposures for Enhanced Decellularization.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Development of a Xenograft for Annular Repair Using Pulsed Electric Field Exposures for Enhanced Decellularization./
作者:	Atsu, Prince Mensah Kwaku.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2023,
面頁冊數:	216 p.
附註:	Source: Dissertations Abstracts International, Volume: 85-07, Section: B.
Contained By:	Dissertations Abstracts International85-07B.
標題:	Chemical engineering. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30568721
ISBN:	9798381379167

Development of a Xenograft for Annular Repair Using Pulsed Electric Field Exposures for Enhanced Decellularization.
Atsu, Prince Mensah Kwaku.

Development of a Xenograft for Annular Repair Using Pulsed Electric Field Exposures for Enhanced Decellularization. - Ann Arbor : ProQuest Dissertations & Theses, 2023 - 216 p.

Source: Dissertations Abstracts International, Volume: 85-07, Section: B.

Thesis (Ph.D.)--Rowan University, 2023.

This item must not be sold to any third party vendors.

Severe back injuries and chronic pain necessitate surgical replacement of damaged intervertebral disc (IVD) cartilage in advanced disease stages. Bovine IVD tissue has been exposed to an upper threshold pulsed electric field (PEF) dose, causing cell death without thermal damage to the tissue. Subsequent PEF exposures at lower magnitudes have accelerated the removal of immunogenic biomolecules though electrokinetic extraction using optimized aqueous solutions. This approach yields a natural scaffold, ready for biocompatibility and mechanical strength assessment. The effects of microsecond pulsed electric fields (µsPEF) on primary bovine AF fibroblast-like cells have been characterized in vitro. PEFs of 10 and 100 µs durations, with varying numbers of pulses and electric field strengths, were applied to the cells. Furthermore, a low-intensity and minimally heating PEF-induced electrokinetic flow for molecular extraction has been established, involving the determination of the electrophoretic mobility of charged molecules within the AF cartilage tissue. Ultimately, a biocompatible AF scaffold has been generated using PEF and electrolyte solutions in a custom-designed bioreactor. Compared to chemical methods that take days, PEF application achieves decellularization of cartilage tissue within three hours, while preserving the desired biomolecules and ultrastructure of the tissue matrix.

ISBN: 9798381379167Subjects--Topical Terms:

560457
Chemical engineering.
Subjects--Index Terms:

Biocompatibility

Development of a Xenograft for Annular Repair Using Pulsed Electric Field Exposures for Enhanced Decellularization.
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300 $a 216 p.
500 $a Source: Dissertations Abstracts International, Volume: 85-07, Section: B.
500 $a Advisor: Yenkie, Kirtie;Thompson, Gary.
502 $a Thesis (Ph.D.)--Rowan University, 2023.
506 $a This item must not be sold to any third party vendors.
520 $a Severe back injuries and chronic pain necessitate surgical replacement of damaged intervertebral disc (IVD) cartilage in advanced disease stages. Bovine IVD tissue has been exposed to an upper threshold pulsed electric field (PEF) dose, causing cell death without thermal damage to the tissue. Subsequent PEF exposures at lower magnitudes have accelerated the removal of immunogenic biomolecules though electrokinetic extraction using optimized aqueous solutions. This approach yields a natural scaffold, ready for biocompatibility and mechanical strength assessment. The effects of microsecond pulsed electric fields (µsPEF) on primary bovine AF fibroblast-like cells have been characterized in vitro. PEFs of 10 and 100 µs durations, with varying numbers of pulses and electric field strengths, were applied to the cells. Furthermore, a low-intensity and minimally heating PEF-induced electrokinetic flow for molecular extraction has been established, involving the determination of the electrophoretic mobility of charged molecules within the AF cartilage tissue. Ultimately, a biocompatible AF scaffold has been generated using PEF and electrolyte solutions in a custom-designed bioreactor. Compared to chemical methods that take days, PEF application achieves decellularization of cartilage tissue within three hours, while preserving the desired biomolecules and ultrastructure of the tissue matrix.
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650 4 $a Chemical engineering. $3 560457
650 4 $a Electrical engineering. $3 649834
650 4 $a Biophysics. $3 518360
653 $a Biocompatibility
653 $a Decellularization
653 $a Electrokinetics
653 $a Electroporation
653 $a Pulsed electric fields
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690 $a 0786
690 $a 0544
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