東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Circular Plastics Economies for Mars...

Pane, Vincent.

FindBook

Google Book

Amazon

博客來

Circular Plastics Economies for Mars and Beyond: 3D Printing a Sustainable Future.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Circular Plastics Economies for Mars and Beyond: 3D Printing a Sustainable Future./
作者:	Pane, Vincent.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2023,
面頁冊數:	304 p.
附註:	Source: Dissertations Abstracts International, Volume: 85-04, Section: B.
Contained By:	Dissertations Abstracts International85-04B.
標題:	Polymers. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30615119
ISBN:	9798380485920

Circular Plastics Economies for Mars and Beyond: 3D Printing a Sustainable Future.
Pane, Vincent.

Circular Plastics Economies for Mars and Beyond: 3D Printing a Sustainable Future. - Ann Arbor : ProQuest Dissertations & Theses, 2023 - 304 p.

Source: Dissertations Abstracts International, Volume: 85-04, Section: B.

Thesis (Ph.D.)--Stanford University, 2023.

This item must not be sold to any third party vendors.

My graduate research explored the synthesis, characterization, and applications of synthetically- and microbially-derived biodegradable polymers. The broad scope of my work was motivated through the NASA institute, CUBES (Center for the Utilization of Biological Engineering in Space). The goal of this group was to use the tools of biological engineering to create a proof-of-concept architecture for a manned mission to Mars. Out of the four interconnected branches that comprised CUBES, my research was part of Biofuel and Biomanufacturing Division (BBMD). This group focused on tool manufacturing, and as such, our goal was to derive polymers through microbial engineering and use them to manufacture useful mission parts via 3D printing.Poly-3-hydroxybutyrate (P3HB) stood out as the most amenable polymer for our purpose, since it is readily available microbially; however, P3HB, has a high crystallinity and a low degradation temperature, making its processing window for manufacturing quite limited. Fused Filament Fabrication (FFF) proved to be the most viable manufacturing option given these constraints, but FFF was still poorly suited for 3D printing P3HB. I engineered solutions for this by using careful temperature control to create uniform printer filament and by altering the print bed surface to control part warping and prevent delamination. Though mission-relevant parts could now be successfully printed using FFF, we were not able to overcome low tolerances on filament production which ultimately constrained the largest printable parts to a few grams.The limitations of 3D printing P3HB helped motivate much of the microbial work of the BBMD in CUBES. Methanotrophs and other microbes rely on sources of CO2, CH4, and O2 for both cell growth and energy storage as P3HB. Understanding the mechanisms by which PHAs (Polyhydroxyalkanoates- a category of polyesters including P3HB) are synthesized microbially can provide valuable insight into how to both maximize PHA accumulation and alter polymer composition. Atmospheric contents for common methanotroph organisms OB3B and OBPB were carefully controlled and monitored under biomass and PHA accumulation conditions to determine the fate of each feedstock gas. Microbes were then fed 13C-labeled CO2 and CH4 to trace their uptake into P3HB and thereby elucidate metabolic features. By 13C NMR, we showed that CO2 contributed to the more oxidized C1 and C3 carbons of the P3HB repeat while the CH4 contributed to the more reduced C2 and C4 carbons. This provided strong support for CH4 incorporation as formate in the serine cycle and CO2utilization in the biosynthesis of oxaloacetate to ultimately form P3HB.With knowledge of the metabolic pathways for P3HB biosynthesis, microbes could be engineered to take up novel substrates. By incorporation of various short-chain fatty acids into the polymer, P3HB's high crystallinity, low elasticity, and narrow processing window are known to be tunable. To broaden the scope of our control over the bio-PHA material properties, Cupriavidus necatorH16 was engineered to uptake novel aryl substrates into the PHA backbone by means of heterologous hydroxyacyl-CoA transferase and mutant PHA synthase. Through NMR studies we showed the first formation of biological polyesters incorporating aromatic rings in the backbone (hydroxyphenylic and a hydroxyfuranoic acid).

ISBN: 9798380485920Subjects--Topical Terms:

535398
Polymers.

Circular Plastics Economies for Mars and Beyond: 3D Printing a Sustainable Future.
LDR:04593nmm a2200397 4500 001 2393833
005 20240604073603.5
006 m o d
007 cr#unu||||||||
008 251215s2023 ||||||||||||||||| ||eng d
020 $a 9798380485920
035 $a (MiAaPQ)AAI30615119
035 $a (MiAaPQ)STANFORDjc009hx4769
035 $a AAI30615119
040 $a MiAaPQ $c MiAaPQ
100 1 $a Pane, Vincent. $3 3763312
245 1 0 $a Circular Plastics Economies for Mars and Beyond: 3D Printing a Sustainable Future.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2023
300 $a 304 p.
500 $a Source: Dissertations Abstracts International, Volume: 85-04, Section: B.
500 $a Advisor: Criddle, Craig;Xia, Yan;Waymouth, Robert.
502 $a Thesis (Ph.D.)--Stanford University, 2023.
506 $a This item must not be sold to any third party vendors.
520 $a My graduate research explored the synthesis, characterization, and applications of synthetically- and microbially-derived biodegradable polymers. The broad scope of my work was motivated through the NASA institute, CUBES (Center for the Utilization of Biological Engineering in Space). The goal of this group was to use the tools of biological engineering to create a proof-of-concept architecture for a manned mission to Mars. Out of the four interconnected branches that comprised CUBES, my research was part of Biofuel and Biomanufacturing Division (BBMD). This group focused on tool manufacturing, and as such, our goal was to derive polymers through microbial engineering and use them to manufacture useful mission parts via 3D printing.Poly-3-hydroxybutyrate (P3HB) stood out as the most amenable polymer for our purpose, since it is readily available microbially; however, P3HB, has a high crystallinity and a low degradation temperature, making its processing window for manufacturing quite limited. Fused Filament Fabrication (FFF) proved to be the most viable manufacturing option given these constraints, but FFF was still poorly suited for 3D printing P3HB. I engineered solutions for this by using careful temperature control to create uniform printer filament and by altering the print bed surface to control part warping and prevent delamination. Though mission-relevant parts could now be successfully printed using FFF, we were not able to overcome low tolerances on filament production which ultimately constrained the largest printable parts to a few grams.The limitations of 3D printing P3HB helped motivate much of the microbial work of the BBMD in CUBES. Methanotrophs and other microbes rely on sources of CO2, CH4, and O2 for both cell growth and energy storage as P3HB. Understanding the mechanisms by which PHAs (Polyhydroxyalkanoates- a category of polyesters including P3HB) are synthesized microbially can provide valuable insight into how to both maximize PHA accumulation and alter polymer composition. Atmospheric contents for common methanotroph organisms OB3B and OBPB were carefully controlled and monitored under biomass and PHA accumulation conditions to determine the fate of each feedstock gas. Microbes were then fed 13C-labeled CO2 and CH4 to trace their uptake into P3HB and thereby elucidate metabolic features. By 13C NMR, we showed that CO2 contributed to the more oxidized C1 and C3 carbons of the P3HB repeat while the CH4 contributed to the more reduced C2 and C4 carbons. This provided strong support for CH4 incorporation as formate in the serine cycle and CO2utilization in the biosynthesis of oxaloacetate to ultimately form P3HB.With knowledge of the metabolic pathways for P3HB biosynthesis, microbes could be engineered to take up novel substrates. By incorporation of various short-chain fatty acids into the polymer, P3HB's high crystallinity, low elasticity, and narrow processing window are known to be tunable. To broaden the scope of our control over the bio-PHA material properties, Cupriavidus necatorH16 was engineered to uptake novel aryl substrates into the PHA backbone by means of heterologous hydroxyacyl-CoA transferase and mutant PHA synthase. Through NMR studies we showed the first formation of biological polyesters incorporating aromatic rings in the backbone (hydroxyphenylic and a hydroxyfuranoic acid).
590 $a School code: 0212.
650 4 $a Polymers. $3 535398
650 4 $a Construction. $3 3561054
650 4 $a Polyesters. $3 686152
650 4 $a Acids. $3 922327
650 4 $a Resins. $3 3682318
650 4 $a Plastics. $3 649803
650 4 $a Polymerization. $3 560492
650 4 $a Copolymers. $3 1073457
650 4 $a 3-D printers. $3 3681750
650 4 $a Chemistry. $3 516420
650 4 $a Design. $3 518875
650 4 $a Molecular weight. $3 3683783
650 4 $a Metabolism. $3 541349
650 4 $a Potassium. $3 3562093
650 4 $a Data replication. $3 3763313
650 4 $a Civil engineering. $3 860360
650 4 $a Planetology. $3 546174
650 4 $a Polymer chemistry. $3 3173488
650 4 $a Sustainability. $3 1029978
690 $a 0485
690 $a 0389
690 $a 0795
690 $a 0543
690 $a 0590
690 $a 0495
690 $a 0640
710 2 $a Stanford University. $3 754827
773 0 $t Dissertations Abstracts International $g 85-04B.
790 $a 0212
791 $a Ph.D.
792 $a 2023
793 $a English
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30615119