東華大學圖書館 |

The Development of a MWCNT-DAO Biosensor for the Detection of Cadaverine in the Assessment of Periodontal Disease.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	The Development of a MWCNT-DAO Biosensor for the Detection of Cadaverine in the Assessment of Periodontal Disease./
作者:	Amin, Mohsin.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2023,
面頁冊數:	220 p.
附註:	Source: Dissertations Abstracts International, Volume: 85-06, Section: B.
Contained By:	Dissertations Abstracts International85-06B.
標題:	Infections. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30736360
ISBN:	9798381026139

The Development of a MWCNT-DAO Biosensor for the Detection of Cadaverine in the Assessment of Periodontal Disease.
Amin, Mohsin.

The Development of a MWCNT-DAO Biosensor for the Detection of Cadaverine in the Assessment of Periodontal Disease. - Ann Arbor : ProQuest Dissertations & Theses, 2023 - 220 p.

Source: Dissertations Abstracts International, Volume: 85-06, Section: B.

Thesis (Ph.D.)--Liverpool John Moores University (United Kingdom), 2023.

This item must not be sold to any third party vendors.

Periodontal disease is one the most common afflictions of human populations and is a major challenge in both the developed and developing world. Cadaverine is a biomolecule which has implications in a myriad of human diseases, in particular periodontal disease. Porphyromonas gingivalishas been suggested as one of the key microorganisms in periodontal disease, and its pathogenesis has been extensively researched. It has a number of metabolites which contribute towards its pathogenicity, and among them, cadaverine has seen particularly detrimental effects in both oral and systemic health. Traditional testing methods for periodontal disease do not provide clinicians with active disease state measurements, but instead provide information on the history of the disease. Thus, the development of a real-time biosensor, providing point-of-care information regarding the metabolite status of the oral cavity would be of significant impact for oral disease diagnostics.This thesis reports the development and testing of a newly designed working electrode fitted to a flexible screen-printed sensor platform for the detection of the biogenic amine, cadaverine. The results in chapter 3 showed that DAO enhanced MWCNT dispersion and increased overall electrode surface topographies, resulting in a more wettable electrode for faster, more efficient electron transfer kinetics. Scanning electron microscopy, UV-Vis, Fourier transform intra-red spectroscopy, raman spectroscopy, and energy diffraction x-ray spectroscopy confirmed the functional group changes the MWCNT's underwent during modification. Investigations into the modified biosensors heterogenous electron transfer rates were carried out and determined an increase to the peak to peak separation, possible due to the additional modification layers on the electrode surface, and the presence of a binder.Chapter 4 investigated electrochemical efficacy of the MWCNT-DAO biosensor against cadaverine in stock solutions, and artificially simulated saliva. The biosensor demonstrated positive concentration dependant correlations towards cadaverine from a range of 3 µg/ml to 150 µg/ml. Similarly in artificial saliva, the biosensors efficacy remained consistent, and presented a potential for the biosensor to function outside of stock sample solutions.The cytotoxic effects of P. gingivalis and cadaverine were investigated in Chapter 5. Initial monocyte differentiation into macrophages was confirmed using flow cytometry. Scanning electron microscopy was used to demonstrate the phagocytic effects of macrophages towards P. gingivalis and resulted in phagocytosis being visualised by the pseudopodia-like appendages engulfing the bacterium. Cell viability, and cell migration assays, and showed significant reductions in the viability and migratory effects of human epithelial keratinocyte and M0 macrophage cells in response to a P. gingivalisinfection, and as a response to increasing cadaverine levels, above the physiological normal thresholds.The findings from this study present a MWCNT-DAO biosensor which was able to detect cadaverine at concentrations which are respective to those in periodontal disease, in stock solutions and in simulated human saliva. Furthermore, the cytotoxic effects of cadaverine and its precursor bacteria, P. gingivaliswere elucidated, and showed significant detrimental effects towards human macrophage and human epithelial keratinocyte cells. thus, the biosensor developed in this study may be used as a tool for determining the extent of a patient's periodontal disease, using a rapid, cost effective and point-of-care biosensor.

ISBN: 9798381026139Subjects--Topical Terms:

1621997
Infections.

The Development of a MWCNT-DAO Biosensor for the Detection of Cadaverine in the Assessment of Periodontal Disease.
LDR:04983nmm a2200433 4500 001 2393708
005 20240414211523.5
006 m o d
007 cr#unu||||||||
008 251215s2023 ||||||||||||||||| ||eng d
020 $a 9798381026139
035 $a (MiAaPQ)AAI30736360
035 $a (MiAaPQ)LiverpoolJohnMoores20519
035 $a AAI30736360
040 $a MiAaPQ $c MiAaPQ
100 1 $a Amin, Mohsin. $3 3763182
245 1 0 $a The Development of a MWCNT-DAO Biosensor for the Detection of Cadaverine in the Assessment of Periodontal Disease.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2023
300 $a 220 p.
500 $a Source: Dissertations Abstracts International, Volume: 85-06, Section: B.
500 $a Advisor: Abdullah, Badr M.;Wylie, Stephen;Whitehead, Kathryn.
502 $a Thesis (Ph.D.)--Liverpool John Moores University (United Kingdom), 2023.
506 $a This item must not be sold to any third party vendors.
520 $a Periodontal disease is one the most common afflictions of human populations and is a major challenge in both the developed and developing world. Cadaverine is a biomolecule which has implications in a myriad of human diseases, in particular periodontal disease. Porphyromonas gingivalishas been suggested as one of the key microorganisms in periodontal disease, and its pathogenesis has been extensively researched. It has a number of metabolites which contribute towards its pathogenicity, and among them, cadaverine has seen particularly detrimental effects in both oral and systemic health. Traditional testing methods for periodontal disease do not provide clinicians with active disease state measurements, but instead provide information on the history of the disease. Thus, the development of a real-time biosensor, providing point-of-care information regarding the metabolite status of the oral cavity would be of significant impact for oral disease diagnostics.This thesis reports the development and testing of a newly designed working electrode fitted to a flexible screen-printed sensor platform for the detection of the biogenic amine, cadaverine. The results in chapter 3 showed that DAO enhanced MWCNT dispersion and increased overall electrode surface topographies, resulting in a more wettable electrode for faster, more efficient electron transfer kinetics. Scanning electron microscopy, UV-Vis, Fourier transform intra-red spectroscopy, raman spectroscopy, and energy diffraction x-ray spectroscopy confirmed the functional group changes the MWCNT's underwent during modification. Investigations into the modified biosensors heterogenous electron transfer rates were carried out and determined an increase to the peak to peak separation, possible due to the additional modification layers on the electrode surface, and the presence of a binder.Chapter 4 investigated electrochemical efficacy of the MWCNT-DAO biosensor against cadaverine in stock solutions, and artificially simulated saliva. The biosensor demonstrated positive concentration dependant correlations towards cadaverine from a range of 3 µg/ml to 150 µg/ml. Similarly in artificial saliva, the biosensors efficacy remained consistent, and presented a potential for the biosensor to function outside of stock sample solutions.The cytotoxic effects of P. gingivalis and cadaverine were investigated in Chapter 5. Initial monocyte differentiation into macrophages was confirmed using flow cytometry. Scanning electron microscopy was used to demonstrate the phagocytic effects of macrophages towards P. gingivalis and resulted in phagocytosis being visualised by the pseudopodia-like appendages engulfing the bacterium. Cell viability, and cell migration assays, and showed significant reductions in the viability and migratory effects of human epithelial keratinocyte and M0 macrophage cells in response to a P. gingivalisinfection, and as a response to increasing cadaverine levels, above the physiological normal thresholds.The findings from this study present a MWCNT-DAO biosensor which was able to detect cadaverine at concentrations which are respective to those in periodontal disease, in stock solutions and in simulated human saliva. Furthermore, the cytotoxic effects of cadaverine and its precursor bacteria, P. gingivaliswere elucidated, and showed significant detrimental effects towards human macrophage and human epithelial keratinocyte cells. thus, the biosensor developed in this study may be used as a tool for determining the extent of a patient's periodontal disease, using a rapid, cost effective and point-of-care biosensor.
590 $a School code: 8357.
650 4 $a Infections. $3 1621997
650 4 $a Physiology. $3 518431
650 4 $a Pathogens. $3 3540520
650 4 $a Food. $3 551593
650 4 $a Electrodes. $3 629151
650 4 $a Cytotoxicity. $3 3682273
650 4 $a Real time. $3 3562675
650 4 $a Bacteria. $3 550366
650 4 $a Flow cytometry. $3 600581
650 4 $a Teeth. $3 1640005
650 4 $a Scanning electron microscopy. $3 551366
650 4 $a Spectrum analysis. $3 520440
650 4 $a Fourier transforms. $3 3545926
650 4 $a Cell adhesion & migration. $3 3561734
650 4 $a Carbon. $3 604057
650 4 $a Medical equipment. $3 3560831
650 4 $a Design. $3 518875
650 4 $a Biomarkers. $3 2205735
650 4 $a Gum disease. $3 3763183
650 4 $a Biosensors. $3 621001
650 4 $a Screen printing. $3 3692486
650 4 $a Enzymes. $3 520899
650 4 $a Microorganisms. $3 666946
650 4 $a Metabolites. $3 683644
650 4 $a Analytical chemistry. $3 3168300
650 4 $a Cellular biology. $3 3172791
650 4 $a Chemistry. $3 516420
650 4 $a Dentistry. $3 828971
650 4 $a Mathematics. $3 515831
650 4 $a Microbiology. $3 536250
650 4 $a Optics. $3 517925
650 4 $a Neurosciences. $3 588700
690 $a 0389
690 $a 0719
690 $a 0486
690 $a 0379
690 $a 0485
690 $a 0567
690 $a 0405
690 $a 0410
690 $a 0752
690 $a 0317
710 2 $a Liverpool John Moores University (United Kingdom). $3 3556313
773 0 $t Dissertations Abstracts International $g 85-06B.
790 $a 8357
791 $a Ph.D.
792 $a 2023
793 $a English
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30736360