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A Modular MRNA Approach to Study and Treat Phospholamban Mediated Cardiomyopathies.

Record Type:	Electronic resources : Monograph/item
Title/Author:	A Modular MRNA Approach to Study and Treat Phospholamban Mediated Cardiomyopathies./
Author:	Rohner, Eduarde.
Description:	1 online resource (60 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 84-04, Section: B.
Contained By:	Dissertations Abstracts International84-04B.
Subject:	Medical research. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29316541click for full text (PQDT)
ISBN:	9798351490830

A Modular MRNA Approach to Study and Treat Phospholamban Mediated Cardiomyopathies.
Rohner, Eduarde.

A Modular MRNA Approach to Study and Treat Phospholamban Mediated Cardiomyopathies. - 1 online resource (60 pages)

Source: Dissertations Abstracts International, Volume: 84-04, Section: B.

Thesis (Ph.D.)--Karolinska Institutet (Sweden), 2022.

Includes bibliographical references

In the heart, phospholamban (PLN) is a key regulator of calcium cycling through itsinhibitory effect on the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) pump. For morethan two decades, modulating calcium channels has been a strong focus of prospectivecardiac therapeutics with a recent emphasis on targeting PLN itself, partially due to theprogressive uncovering of several cardiomyopathic PLN human mutations. Ourunderstanding of the physiological and pathophysiological mechanisms regulating ormisregulating calcium cycling in humans in not fully understood. Therefore, careful study ofnaturally occurring human mutations can offer important insights into these mechanisms.This thesis focuses on developing novel investigative and potential therapeutic tools tomechanically dissect and treat phospholamban mediated cardiomyopathies.The constituent papers compiled in this thesis validates these novel and innovativeapproaches which utilize an mRNA modular system to model and dissect core mechanisticdifferences caused by cardiomyopathic phospholamban mutations in embryonic stem cellderived cardiomyocytes. Additionally, this thesis showcases the usage of single-chain VHHantibodies (intrabodies) to express, target and modulate PLN intracellularly in vitro and in vivo with promising results validating its usage as a potential therapy and valuableinvestigative tool.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798351490830Subjects--Topical Terms:

1556686
Medical research.
Index Terms--Genre/Form:

542853
Electronic books.

A Modular MRNA Approach to Study and Treat Phospholamban Mediated Cardiomyopathies.
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100 1 $a Rohner, Eduarde. $3 3706554
245 1 2 $a A Modular MRNA Approach to Study and Treat Phospholamban Mediated Cardiomyopathies.
264 0 $c 2022
300 $a 1 online resource (60 pages)
336 $a text $b txt $2 rdacontent
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500 $a Source: Dissertations Abstracts International, Volume: 84-04, Section: B.
500 $a Advisor: Chien, Kenneth R. ; Sahara, Makoto ; Santoro, Federica ; Louch, William E.
502 $a Thesis (Ph.D.)--Karolinska Institutet (Sweden), 2022.
504 $a Includes bibliographical references
520 $a In the heart, phospholamban (PLN) is a key regulator of calcium cycling through itsinhibitory effect on the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) pump. For morethan two decades, modulating calcium channels has been a strong focus of prospectivecardiac therapeutics with a recent emphasis on targeting PLN itself, partially due to theprogressive uncovering of several cardiomyopathic PLN human mutations. Ourunderstanding of the physiological and pathophysiological mechanisms regulating ormisregulating calcium cycling in humans in not fully understood. Therefore, careful study ofnaturally occurring human mutations can offer important insights into these mechanisms.This thesis focuses on developing novel investigative and potential therapeutic tools tomechanically dissect and treat phospholamban mediated cardiomyopathies.The constituent papers compiled in this thesis validates these novel and innovativeapproaches which utilize an mRNA modular system to model and dissect core mechanisticdifferences caused by cardiomyopathic phospholamban mutations in embryonic stem cellderived cardiomyocytes. Additionally, this thesis showcases the usage of single-chain VHHantibodies (intrabodies) to express, target and modulate PLN intracellularly in vitro and in vivo with promising results validating its usage as a potential therapy and valuableinvestigative tool.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2023
538 $a Mode of access: World Wide Web
650 4 $a Medical research. $2 bicssc $3 1556686
650 4 $a Physiology. $3 518431
650 4 $a Medicine. $3 641104
655 7 $a Electronic books. $2 lcsh $3 542853
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710 2 $a Karolinska Institutet (Sweden). $3 3557748
773 0 $t Dissertations Abstracts International $g 84-04B.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29316541 $z click for full text (PQDT)