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Deciphering Hidden Mechanisms in the Biomagnetic Response in Plants : = A Study on the Effects of Magnetic Fields on Plant Growth, Development, and Molecular Responses.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Deciphering Hidden Mechanisms in the Biomagnetic Response in Plants :/
Reminder of title:	A Study on the Effects of Magnetic Fields on Plant Growth, Development, and Molecular Responses.
Author:	Lockett, Andrea.
Description:	1 online resource (90 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 84-11, Section: B.
Contained By:	Dissertations Abstracts International84-11B.
Subject:	Plant sciences. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30488162click for full text (PQDT)
ISBN:	9798379571634

Deciphering Hidden Mechanisms in the Biomagnetic Response in Plants : = A Study on the Effects of Magnetic Fields on Plant Growth, Development, and Molecular Responses.
Lockett, Andrea.

Deciphering Hidden Mechanisms in the Biomagnetic Response in Plants :A Study on the Effects of Magnetic Fields on Plant Growth, Development, and Molecular Responses. - 1 online resource (90 pages)

Source: Dissertations Abstracts International, Volume: 84-11, Section: B.

Thesis (M.S.)--Tuskegee University, 2023.

Includes bibliographical references

Plants sense and respond to environmental stimuli (light wavelengths, gravity, touch, electromagnetic stimulation) with alterations at the molecular level, which are expressed through physiological changes in growth and development. Earth's magnetic field also known as geomagnetic field (GMF) are consistent environmental stimuli. The GMF is generated by the rotation of Earth's iron core, which conducts electricity. Plants exposed to magnetic fields have shown a variety of physiological responses such as an increase in seed germination, plant growth, pigment synthesis, water and nutrients uptake, and alterations in the expression of proteins and enzymes. The effects of the Earth's magnetic fields on plants' molecular responses have not been well documented. Thus, a comprehensive understanding of magneto-reception dynamism in plants necessitates more in-depth approaches. In this study tomato (S. lycopersicum) and collard (B. oleracea var. viridis) seeds exposed to a magnetic field intensity of 4.7 Gauss were evaluated through phenotypic screenings of physio morphological changes, HPLC-LCMS analysis of metabolite expression and transcriptome profiling using RNA-sequencing. The objectives are: 1.) To screen plant growth responses to magnetic field exposure based on selected physio-morphological growth parameters; 2.) To screen plant metabolomics responses and the secondary metabolite expression in control versus exposed to magnetic fields through high-performance liquid chromatography-mass spectrometry (HPLC-MS). 3.) Identify the effects of magnetic fields in plants by transcriptome profiling to characterize genetic responses, and potential differential gene expression patterns in exposed plants. Seeds were sterilized and placed into for MFE. Seeds were exposed at 4.33 gauss plus the local GMF, for a total of 4.7 Gauss over a period of six days for two hours a day. The experiment followed a randomized block design with three replicants (petri dishes) per objective, repeated for two trials Physio Morphological- Following exposures, collards and tomatoes seeds were planted in potting soil under greenhouse conditions as growth parameters including stem diameter, chlorophyll concentrations, and plant height were recorded weekly for six weeks . The statistical results concluded that there was no significance in all growth parameters between control and exposed groups (p<0.05).. Metabolomic Analysis-Metabolomic extraction was completed immediately after exposures ended on the sixth day. Following a methanol-based metabolite extraction, metabolomics analysis was performed by HPLC-MS. The raw data received from HPLC-MS analysis was then analyzed using Metaboanalyst (https://www.metaboanalyst.ca/). Using Pathway and Enrichment analysis, 140 compounds were identified and grouped into metabolite pathways through hypergeometric analysis (p < 0.05). Linoleic acid metabolism was the highest expressed metabolic pathway p < 0.00511) in all trials for tomatoes and collards. The identified significant metabolites (p < 0.05) in both trials collards and tomatoes were C3 H N3 O3 P2 and pantothenic acid, respectively. Transcriptome Profiling- RNA extraction of control and exposed tomato samples was performed for RNA-sequencing analysis. Differential gene expression analysis was performed by the quantitation of mapped reads, dictated by FPKM values. For trial 1,2 control and 1,2 exposed 509, 434, 409, and 524 genes were differentially expressed, respectively. Exposure resulted in the down regulation of five novel genes, Solyc07g065840.2, a heat shock gene, Solyc09g010630.3, Solyc01g099770.3, Solyc01g101060.3, and Solyc03g119080.3. The proposed study can contribute to the fundamental understanding of magnetic field effects on plant development through a comprehensive approach, and the development of specific MFE protocols may alter the expression of important biosynthetic pathways and products. Keywords: biomagnetism, plants, molecular and transcriptome profiling, magnetic fields.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798379571634Subjects--Topical Terms:

3173832
Plant sciences.
Subjects--Index Terms:

Biomagnetic responseIndex Terms--Genre/Form:

542853
Electronic books.

Deciphering Hidden Mechanisms in the Biomagnetic Response in Plants : = A Study on the Effects of Magnetic Fields on Plant Growth, Development, and Molecular Responses.
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