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Optogenetic Engineering of Bar Domain Proteins.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Optogenetic Engineering of Bar Domain Proteins./
Author:	Jones, Taylor.
Description:	1 online resource (183 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 85-04, Section: B.
Contained By:	Dissertations Abstracts International85-04B.
Subject:	Membranes. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30615133click for full text (PQDT)
ISBN:	9798380485890

Optogenetic Engineering of Bar Domain Proteins.
Jones, Taylor.

Optogenetic Engineering of Bar Domain Proteins. - 1 online resource (183 pages)

Source: Dissertations Abstracts International, Volume: 85-04, Section: B.

Thesis (Ph.D.)--Stanford University, 2023.

Includes bibliographical references

Nanoscale membrane curvature is understood to play an active role in essential cellular processes such as endocytosis, exocytosis, and actin dynamics. Few methods, however, can precisely manipulate membrane curvature in live cells. Making use of BAR domain proteins, a well-studied superfamily of membraneremodeling proteins, and the improved Light-Inducible Dimer (iLID) system, we developed a new method of generating nanoscale membrane curvature in live cells that is controllable, reversible, and capable of precise spatial and temporal manipulation. As proof of concept, we first engineered two optogenetic systems, opto-FBAR and opto-IBAR, that allow light-inducible formation of inward and outward membrane curvature, respectively. We then expanded upon this approach by engineering other BAR domain superfamily members to be light-inducible, including members of the FCHO, PACSIN, Amphiphysin, and NOSTRIN subfamilies. These systems present a novel approach for light-inducible manipulation of nanoscale membrane curvature in live cells.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798380485890Subjects--Topical Terms:

1531702
Membranes.
Index Terms--Genre/Form:

542853
Electronic books.

Optogenetic Engineering of Bar Domain Proteins.
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100 1 $a Jones, Taylor. $3 3703834
245 1 0 $a Optogenetic Engineering of Bar Domain Proteins.
264 0 $c 2023
300 $a 1 online resource (183 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertations Abstracts International, Volume: 85-04, Section: B.
500 $a Advisor: Cui, Bianxiao.
502 $a Thesis (Ph.D.)--Stanford University, 2023.
504 $a Includes bibliographical references
520 $a Nanoscale membrane curvature is understood to play an active role in essential cellular processes such as endocytosis, exocytosis, and actin dynamics. Few methods, however, can precisely manipulate membrane curvature in live cells. Making use of BAR domain proteins, a well-studied superfamily of membraneremodeling proteins, and the improved Light-Inducible Dimer (iLID) system, we developed a new method of generating nanoscale membrane curvature in live cells that is controllable, reversible, and capable of precise spatial and temporal manipulation. As proof of concept, we first engineered two optogenetic systems, opto-FBAR and opto-IBAR, that allow light-inducible formation of inward and outward membrane curvature, respectively. We then expanded upon this approach by engineering other BAR domain superfamily members to be light-inducible, including members of the FCHO, PACSIN, Amphiphysin, and NOSTRIN subfamilies. These systems present a novel approach for light-inducible manipulation of nanoscale membrane curvature in live cells.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2023
538 $a Mode of access: World Wide Web
650 4 $a Membranes. $3 1531702
650 4 $a Plasmids. $3 3319070
650 4 $a Signal transduction. $3 3546420
650 4 $a Plasma. $3 877619
650 4 $a Cell adhesion & migration. $3 3561734
650 4 $a Sensors. $3 3549539
650 4 $a Microscopy. $3 540544
650 4 $a Engineering. $3 586835
650 4 $a E coli. $3 3556834
650 4 $a Lipids. $3 558980
650 4 $a Deformation. $2 lcstt $3 3267001
650 4 $a Cell culture. $3 604671
650 4 $a Cellular biology. $3 3172791
655 7 $a Electronic books. $2 lcsh $3 542853
690 $a 0537
690 $a 0379
710 2 $a ProQuest Information and Learning Co. $3 783688
710 2 $a Stanford University. $3 754827
773 0 $t Dissertations Abstracts International $g 85-04B.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30615133 $z click for full text (PQDT)