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Uncovering Reproductive Roles of Cell Wall Glycoproteins in Flowering Plants.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Uncovering Reproductive Roles of Cell Wall Glycoproteins in Flowering Plants./
作者:	Lara Mondragon, Cecilia M.
面頁冊數:	1 online resource (189 pages)
附註:	Source: Dissertations Abstracts International, Volume: 84-04, Section: B.
Contained By:	Dissertations Abstracts International84-04B.
標題:	Cellular biology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29712265click for full text (PQDT)
ISBN:	9798845451910

Uncovering Reproductive Roles of Cell Wall Glycoproteins in Flowering Plants.
Lara Mondragon, Cecilia M.

Uncovering Reproductive Roles of Cell Wall Glycoproteins in Flowering Plants. - 1 online resource (189 pages)

Source: Dissertations Abstracts International, Volume: 84-04, Section: B.

Thesis (Ph.D.)--University of Michigan, 2022.

Includes bibliographical references

Plant cells are surrounded by a polysaccharide-rich extracellular matrix, the cell wall. The cell wall constitutes the interphase of interaction between the cell and its surrounding environment, as well as a medium for cell-cell communication within plant tissues. In addition to polysaccharides, the wall hosts a vast array of proteins with putative roles in these processes, although characterization of such functions remains to be determined. Comparative studies indicate that evolutionary milestones in the land plant clade (e.g., transition to land, flowering plant radiation) correlate with the expansion in the number of cell wall genes; trend also observed in the most abundant group of wall proteins, the Hydroxyproline-rich glycoprotein family (HRGP). While most of the currently known functions of canonical HRGPs are related to cell wall architecture, the existence of a plethora of noncanonical HRGPs (chimeras and hybrids) indicate potential functional diversification within the family. The expansion of the HRGP family, particularly during the radiation of the flowering plants (angiosperms), suggests the emergence of novel functions related to processes exclusive to angiosperms. Angiosperms are the most abundant and diverse group in the plant kingdom and their current ecological success has been, in part, associated to their unique sexual reproduction mechanism: immobile sperm cells are transported by a remarkably fast-growing structure, the pollen tube, to receptive ovules deeply embedded in the maternal tissues of the pistil. Since angiosperm sexual reproduction involve, on one hand, rapid cell growth by the pollen tube to deliver male gametes and, on the other hand, the establishment of cell-cell communication as the pollen tube traverses the female tissues, we investigated the participation of noncanonical HRGPs during both these processes. On the male side, our research sheds light on the function of two HRGP chimeras, the Arabidopsis class I formins AtFH3 and AtFH5, acting as molecular linkers between actin cytoskeleton and the cell wall during pollen tube elongation. Our results revealed that the extracellular domain of AtFH3 and AtFH5, which contain distinct HRGP-like O-glycans, plays an important role in spatially constraining these proteins to specific plasma membrane domains and consequently, contributing to the regulation of their respective actin nucleation activities. On the female side, our results indicate that HRGP accumulation are correlated with the establishment of receptivity in tomato pistils. Through a combination of immunological methods and high throughput mRNA sequencing, we identified novel noncanonical HRGPs with potential functions supporting pollen tube growth through the pistil and/or as guidance cues for ovular targeting. Together, our findings constitute a step forward to understanding the functional specialization of noncanonical HRGPs during reproductive processes in flowering plants.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798845451910Subjects--Topical Terms:

3172791
Cellular biology.
Subjects--Index Terms:

Reproductive biologyIndex Terms--Genre/Form:

542853
Electronic books.

Uncovering Reproductive Roles of Cell Wall Glycoproteins in Flowering Plants.
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504 $a Includes bibliographical references
520 $a Plant cells are surrounded by a polysaccharide-rich extracellular matrix, the cell wall. The cell wall constitutes the interphase of interaction between the cell and its surrounding environment, as well as a medium for cell-cell communication within plant tissues. In addition to polysaccharides, the wall hosts a vast array of proteins with putative roles in these processes, although characterization of such functions remains to be determined. Comparative studies indicate that evolutionary milestones in the land plant clade (e.g., transition to land, flowering plant radiation) correlate with the expansion in the number of cell wall genes; trend also observed in the most abundant group of wall proteins, the Hydroxyproline-rich glycoprotein family (HRGP). While most of the currently known functions of canonical HRGPs are related to cell wall architecture, the existence of a plethora of noncanonical HRGPs (chimeras and hybrids) indicate potential functional diversification within the family. The expansion of the HRGP family, particularly during the radiation of the flowering plants (angiosperms), suggests the emergence of novel functions related to processes exclusive to angiosperms. Angiosperms are the most abundant and diverse group in the plant kingdom and their current ecological success has been, in part, associated to their unique sexual reproduction mechanism: immobile sperm cells are transported by a remarkably fast-growing structure, the pollen tube, to receptive ovules deeply embedded in the maternal tissues of the pistil. Since angiosperm sexual reproduction involve, on one hand, rapid cell growth by the pollen tube to deliver male gametes and, on the other hand, the establishment of cell-cell communication as the pollen tube traverses the female tissues, we investigated the participation of noncanonical HRGPs during both these processes. On the male side, our research sheds light on the function of two HRGP chimeras, the Arabidopsis class I formins AtFH3 and AtFH5, acting as molecular linkers between actin cytoskeleton and the cell wall during pollen tube elongation. Our results revealed that the extracellular domain of AtFH3 and AtFH5, which contain distinct HRGP-like O-glycans, plays an important role in spatially constraining these proteins to specific plasma membrane domains and consequently, contributing to the regulation of their respective actin nucleation activities. On the female side, our results indicate that HRGP accumulation are correlated with the establishment of receptivity in tomato pistils. Through a combination of immunological methods and high throughput mRNA sequencing, we identified novel noncanonical HRGPs with potential functions supporting pollen tube growth through the pistil and/or as guidance cues for ovular targeting. Together, our findings constitute a step forward to understanding the functional specialization of noncanonical HRGPs during reproductive processes in flowering plants.
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