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Imaging Live Drosophila Brain with Two-Photon Fluorescence Microscopy.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Imaging Live Drosophila Brain with Two-Photon Fluorescence Microscopy./
Author:	Ahmed, Syeed Ehsan.
Description:	1 online resource (67 pages)
Notes:	Source: Masters Abstracts International, Volume: 79-06.
Contained By:	Masters Abstracts International79-06.
Subject:	Physics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10616944click for full text (PQDT)
ISBN:	9780355261004

Imaging Live Drosophila Brain with Two-Photon Fluorescence Microscopy.
Ahmed, Syeed Ehsan.

Imaging Live Drosophila Brain with Two-Photon Fluorescence Microscopy. - 1 online resource (67 pages)

Source: Masters Abstracts International, Volume: 79-06.

Thesis (M.S.)--The University of Texas at El Paso, 2017.

Includes bibliographical references

Two-photon fluorescence microscopy is an imaging technique which delivers distinct benefits for in vivo cellular and molecular imaging. Cyclic adenosine monophosphate (cAMP), a second messenger molecule, is responsible for triggering many physiological changes in neural system. However, the mechanism by which this molecule regulates responses in neuron cells is not yet clearly understood. When cAMP binds to a target protein, it changes the structure of that protein. Therefore, studying this molecular structure change with fluorescence resonance energy transfer (FRET) imaging can shed light on the cAMP functioning mechanism. FRET is a non-radiative dipole-dipole coupling which is sensitive to small distance change in nanometer scale. In this study we have investigated the effect of dopamine in cAMP dynamics in vivo. In our study two-photon fluorescence microscope was used for imaging mushroom bodies inside live Drosophila melanogaster brain and we developed a method for studying the change in cyclic AMP level.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9780355261004Subjects--Topical Terms:

516296
Physics.
Subjects--Index Terms:

Cyclic adenosine monophosphateIndex Terms--Genre/Form:

542853
Electronic books.

Imaging Live Drosophila Brain with Two-Photon Fluorescence Microscopy.
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500 $a Source: Masters Abstracts International, Volume: 79-06.
500 $a Publisher info.: Dissertation/Thesis.
500 $a Advisor: Li, Chunqiang.
502 $a Thesis (M.S.)--The University of Texas at El Paso, 2017.
504 $a Includes bibliographical references
520 $a Two-photon fluorescence microscopy is an imaging technique which delivers distinct benefits for in vivo cellular and molecular imaging. Cyclic adenosine monophosphate (cAMP), a second messenger molecule, is responsible for triggering many physiological changes in neural system. However, the mechanism by which this molecule regulates responses in neuron cells is not yet clearly understood. When cAMP binds to a target protein, it changes the structure of that protein. Therefore, studying this molecular structure change with fluorescence resonance energy transfer (FRET) imaging can shed light on the cAMP functioning mechanism. FRET is a non-radiative dipole-dipole coupling which is sensitive to small distance change in nanometer scale. In this study we have investigated the effect of dopamine in cAMP dynamics in vivo. In our study two-photon fluorescence microscope was used for imaging mushroom bodies inside live Drosophila melanogaster brain and we developed a method for studying the change in cyclic AMP level.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2023
538 $a Mode of access: World Wide Web
650 4 $a Physics. $3 516296
650 4 $a Optics. $3 517925
650 4 $a Biophysics. $3 518360
653 $a Cyclic adenosine monophosphate
653 $a Dopamine
653 $a Fluorescence resonance energy transfer
653 $a Two-photon fluorescence microscopy
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