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Enhancing Transglycosylation Reaction by Minimizing Hydrolysis in Oligosaccharide Synthesis.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Enhancing Transglycosylation Reaction by Minimizing Hydrolysis in Oligosaccharide Synthesis./
作者:	Kwamboka, Charity Maosah.
面頁冊數:	1 online resource (88 pages)
附註:	Source: Masters Abstracts International, Volume: 83-02.
Contained By:	Masters Abstracts International83-02.
標題:	Chemistry. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28678863click for full text (PQDT)
ISBN:	9798534683820

Enhancing Transglycosylation Reaction by Minimizing Hydrolysis in Oligosaccharide Synthesis.
Kwamboka, Charity Maosah.

Enhancing Transglycosylation Reaction by Minimizing Hydrolysis in Oligosaccharide Synthesis. - 1 online resource (88 pages)

Source: Masters Abstracts International, Volume: 83-02.

Thesis (M.S.)--Youngstown State University, 2021.

Includes bibliographical references

Oligosaccharides are a class of carbohydrates which play important roles in several areas including therepeutics, food, phamaceuticals, animal feed and their use as prebiotics. Current commercial production of oligosaccharides involves lengthy multistep procedures with lowbyields. To improve oligosaccharide yield, a broad range of factors related to oligosaccharide synthesis by transglycosylation was evaluated. Three enzymes, β-galactosidase from Aspergillus oryzae, lactase from lactose intolerance supplements, and Enterobacter β-galactosidase were tested for their ability to catalyze a transglycosylation reaction. The enzymes were applied in both free and immobilized forms and the activities toward transglycosylation were compared. Immobilization of enzymes led to increased oligosaccharide synthesis and improved enzyme stability under different conditions. Lactose, which is a main milk carbohydrate, was used as the major substrate for synthesis of oligosaccharides. Factors like organic solvents, temperature, and pH were varied with the aim of obtaining the conditions with higher transglycosylation results. The analysis of products was done by thin layer chromatography. The yield of transglycosylation products was dependent on both temperature and pH. The preferred conditions for formation of oligosaccharides are pH 8 and 50 - 60 °C. High concentrations of glucose and galactose, the products of lactose hydrolysis, suppressed both transglycosylation and hydrolysis reactions. When sucrose and lactose were used together, an increase in the sucrose to lactose ratio led to an increase in transglycosylation products. Addition of alcohols resulted in formation of alkyl-glycosides instead of oligosaccharides. Enterobacter β-galactosidase was successfully overexpressed in E. coli cells and demonstrated transglycosylation and hydrolysis activity.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798534683820Subjects--Topical Terms:

516420
Chemistry.
Subjects--Index Terms:

TransglycosylationIndex Terms--Genre/Form:

542853
Electronic books.

Enhancing Transglycosylation Reaction by Minimizing Hydrolysis in Oligosaccharide Synthesis.
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538 $a Mode of access: World Wide Web
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650 4 $a Biochemistry. $3 518028
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650 4 $a Artificial sweeteners. $3 3700859
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